Application
Western blot
Sample
Mouse Cell lysate - whole cell (Mouse macrophage cell line)
Gel Running Conditions
Reduced Denaturing (15)
Loading amount
5 µg
Specification
Mouse macrophage cell line
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA in TBST
Dilution
1/1000
Secondary antibody
Secondary antibody
None used
Detection
Detection method
ECL
Exposure
20 second(s)
Bands
Specific: 17 kDa
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提交于 Nov 18 2020