产品名称Histone H3 (di-methyl K4) Quantification试剂盒(Colorimetric)
参阅全部 Histone H3 (K4 methylation) 试剂盒
样品类型Tissue, Adherent cells, Suspension cells
种属反应性与反应: Mouse, Human
Methylation of histone H3 at lysine 4 by Methyl transferases such as SET1, SET7/9, MLL and Trx among others occurs in mammals in several distinct genomic distributions. H3 (di-methyl K4) is associated with transcriptionally permissive euchromatin regions in the genome; in particular, Enrichment of H3 (di-methyl K4) is present across the body of genes, where contributes to mRNA processing.
Histone H3 (di-methyl K4) Quantification Kit (Colorimetric) (ab115052) allows the user to specifically measure global di-methylation of histone H3K4 colorimetrically, using a variety of mammalian cells (human, mouse, etc.) including fresh and frozen tissues, cultured adherent and suspension cells.
存放说明Please refer to protocols.
组件 48 tests 96 tests 10X Wash Buffer 1 x 10ml 1 x 20ml 8-Well Sample Strips (with frame) 4 units 9 units 8-Well Standard Control Strips 2 units 3 units Antibody Buffer 1 x 6ml 1 x 12ml Color Developer 1 x 5ml 1 x 10ml Detection Antibody, 1 mg/mL 1 x 5µl 1 x 10µl Standard Control (100 µg/mL) 1 x 10µl 1 x 20µl Stop Solution 1 x 3ml 1 x 6ml
Histone extracts were prepared from MDA-231 cells using the Histone Extraction Kit (ab113476) and the amount of di-methyl-H3-K4 was measured using ab115052.
ab115052 has not yet been referenced specifically in any publications.