Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (Mouse brain after stroke)
Antigen retrieval step
None
Permeabilization
No
Specification
Mouse brain after stroke
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% NHS in T-PBS (Triton X-100: 0,25%)
Dilution
1/300
Secondary antibody
Dilution
1/200
Name
Non-Abcam antibody was used: Horse Anti-Rabbit IgG Antibody (H+L), DyLight® 594
Host species: Horse
Clonality: Polyclonal
Conjugation: DyLight® 594
Host species: Horse
Clonality: Polyclonal
Conjugation: DyLight® 594
Additional data
Additional Notes
Perfusion with 4% PFA was followed by overnight postfixation, also in 4% PFA.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Feb 10 2021