Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (pulmonary alveolar macrophages and epithelial cell)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH6 30min
Permeabilization
No
Specification
pulmonary alveolar macrophages and epithelial cell
Blocking step
Serum as blocking agent for 10 minute(s) · Concentration: 1.5% · Temperature: 24°C
Fixative
Formaldehyde
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: blocking reagent
Dilution
1/100
Secondary antibody
Name
Non-Abcam antibody was used: Histofine® Simple Stain™ Rat MAX PO (MULTI)
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Nov 11 2020