Application
Western blot
Sample
Platynereis dumerilii Tissue lysate - whole (entire organism)
Gel Running Conditions
Reduced Denaturing (14%)
Loading amount
5 µg
Specification
entire organism
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 3% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: BSA 4% in TBST
Dilution
1/2000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: Ab EasyBlot anti-Rabbit IgG (HRP) GTX221666-01-S
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Host species: Mouse
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
HRP enhanced luminol-based chemiluminesc
Negative control
none
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 17 kDa Non-specific: 12 kDa
Positive control
none
Additional data
Additional Notes
Image refers to 6 biological replicates from 7 animal each replicate
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
PROF. Camilla Della Torre
Verified customer
提交于 Jun 10 2021