Application
Western blot
Sample
Schmidtea mediterranea Cell lysate - whole cell (Whole worm)
Gel Running Conditions
Reduced Denaturing (Invitrogen™ NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gels, 1.0 mm, 10-well)
Loading amount
15 µg
Specification
Whole worm
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Detection
Detection method
ECL
Negative control
BSA
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: -17kDA kDa
Positive control
HT1080 cell lysate
S.med lysate
Additional data
Additional Notes
Lanes:
1. S.med lysate
2. HT1080 lysate
3. Negative control
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提交于 Oct 22 2018