Application
Western blot
Loading amount
1 µg
Gel Running Conditions
Reduced Denaturing (18 % SDS-PAGE)
Sample
Dictyostelium discoideum Purified protein (Vegetative cells)
Specification
Vegetative cells
Blocking step
No blocking step used for 30 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/3000
Secondary antibody
Name
Abcam antibody:Goat Anti-Rabbit IgG H&L (HRP)
Detection
Detection method
ECL+
Bands
Specific: 15.6 kDa
Exposure
4 minute(s) and 0 second(s)
Positive control
Mouse histone extract
Additional data
Additional Notes
In Dictyostelium histone H3b variant is preferentially acetylated at K9. This antibody can recognize similar patter as in Nucleic Acids Res. 40, 7247-7256.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Duen-Wei Hsu
Verified customer
提交于 Mar 31 2014