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Epigenetics and Nuclear Signaling Histones H3 Acetylated

Anti-Histone H3 (acetyl K9)抗体- ChIP Grade (ab4441)

  • Datasheet
  • SDS
Reviews (38)Q&A (8)References (317)

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ChIP - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)
  • ChIP - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)
  • Western blot - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)
  • Western blot - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)

Key features and details

  • Rabbit polyclonal to Histone H3 (acetyl K9) - ChIP Grade
  • Suitable for: WB, ChIP
  • Reacts with: Mouse, Human
  • Isotype: IgG

重组抗体的批间可重复性更强

Product image
Anti-Histone H3 (acetyl K9) antibody [Y28] - ChIP Grade (ab32129)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-Histone H3 (acetyl K9)抗体- ChIP Grade
    参阅全部 Histone H3 一抗
  • 描述

    兔多克隆抗体to Histone H3 (acetyl K9) - ChIP Grade
  • 宿主

    Rabbit
  • 特异性

    In Dot blot detects 50ng of mono-acetylated peptide corresponding to position Lys9 in the N-terminal sequence of Histone H3. Does not detect the mono-acetylated peptide corresponding to acetyl-lysine at position 14 or unacetylated Histone H3.
  • 经测试应用

    适用于: WB, ChIPmore details
  • 种属反应性

    与反应: Mouse, Human
    预测可用于: Saccharomyces cerevisiae
  • 免疫原

    Synthetic peptide corresponding to Human Histone H3 aa 1-12 (acetyl K9) conjugated to Keyhole Limpet Haemocyanin (KLH).
    Sequence:

    ARTKQTAR(Ac)KSTG-C

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 阳性对照

    • ChIP: U2OS cells, Human Cell lysate - nuclear (Primary CD34+ cells) WB: Mouse MEL cell nuclear lysate, HeLa cell acid-extract
  • 常规说明

    Learn about ChIP assay kits, other ChIP antibodies, protocols and more in the ChIP assay guide.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.05% Sodium azide
    Constituents: 0.184% Tris glycine, 30% Glycerol, 0.87% Sodium chloride
  • Concentration information loading...
  • 纯度

    Protein A purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Histones
    • H3
    • Acetylated
    • Epigenetics and Nuclear Signaling
    • ChIP assays
    • ChIP antibodies

相关产品

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Positive Controls

    • GAPDH positive control ChIP primer pair (ab267832)
  • Recombinant Protein

    • Recombinant human Histone H3 protein (Active) (ab198757)
  • Related Products

    • Histone H3 (acetyl K9) Quantification Kit (Colorimetric) (ab115104)
    • Prestained Protein Ladder - Broad molecular weight (10-245 kDa) (ab116028)
    • Human Histone H3 (tri methyl K4) peptide (ab1342)
    • Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) (ab156064)
    • Human Histone H3 (tri methyl K9) peptide (ab1773)
    • Human Histone H3 (tri methyl K27) peptide (ab1782)
    • Histone Acetyltransferase Activity Assay Kit (Colorimetric) (ab65352)
    • Human Histone H3 (unmodified) peptide (ab7228)
    • Human Histone H3 (di methyl K4) peptide (ab7768)

应用

Our Abpromise guarantee covers the use of ab4441 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB 1/10000. Detects a band of approximately 17 kDa.
ChIP Use 2µg for 106 cells.

Use GAPDH primer pair ab267832 as positive control.

靶标

  • 功能

    Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
  • 序列相似性

    Belongs to the histone H3 family.
  • 发展阶段

    Expressed during S phase, then expression strongly decreases as cell division slows down during the process of differentiation.
  • 翻译后修饰

    Acetylation is generally linked to gene activation. Acetylation on Lys-10 (H3K9ac) impairs methylation at Arg-9 (H3R8me2s). Acetylation on Lys-19 (H3K18ac) and Lys-24 (H3K24ac) favors methylation at Arg-18 (H3R17me).
    Citrullination at Arg-9 (H3R8ci) and/or Arg-18 (H3R17ci) by PADI4 impairs methylation and represses transcription.
    Asymmetric dimethylation at Arg-18 (H3R17me2a) by CARM1 is linked to gene activation. Symmetric dimethylation at Arg-9 (H3R8me2s) by PRMT5 is linked to gene repression. Asymmetric dimethylation at Arg-3 (H3R2me2a) by PRMT6 is linked to gene repression and is mutually exclusive with H3 Lys-5 methylation (H3K4me2 and H3K4me3). H3R2me2a is present at the 3' of genes regardless of their transcription state and is enriched on inactive promoters, while it is absent on active promoters.
    Methylation at Lys-5 (H3K4me), Lys-37 (H3K36me) and Lys-80 (H3K79me) are linked to gene activation. Methylation at Lys-5 (H3K4me) facilitates subsequent acetylation of H3 and H4. Methylation at Lys-80 (H3K79me) is associated with DNA double-strand break (DSB) responses and is a specific target for TP53BP1. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are linked to gene repression. Methylation at Lys-10 (H3K9me) is a specific target for HP1 proteins (CBX1, CBX3 and CBX5) and prevents subsequent phosphorylation at Ser-11 (H3S10ph) and acetylation of H3 and H4. Methylation at Lys-5 (H3K4me) and Lys-80 (H3K79me) require preliminary monoubiquitination of H2B at 'Lys-120'. Methylation at Lys-10 (H3K9me) and Lys-28 (H3K27me) are enriched in inactive X chromosome chromatin.
    Phosphorylated at Thr-4 (H3T3ph) by GSG2/haspin during prophase and dephosphorylated during anaphase. Phosphorylation at Ser-11 (H3S10ph) by AURKB is crucial for chromosome condensation and cell-cycle progression during mitosis and meiosis. In addition phosphorylation at Ser-11 (H3S10ph) by RPS6KA4 and RPS6KA5 is important during interphase because it enables the transcription of genes following external stimulation, like mitogens, stress, growth factors or UV irradiation and result in the activation of genes, such as c-fos and c-jun. Phosphorylation at Ser-11 (H3S10ph), which is linked to gene activation, prevents methylation at Lys-10 (H3K9me) but facilitates acetylation of H3 and H4. Phosphorylation at Ser-11 (H3S10ph) by AURKB mediates the dissociation of HP1 proteins (CBX1, CBX3 and CBX5) from heterochromatin. Phosphorylation at Ser-11 (H3S10ph) is also an essential regulatory mechanism for neoplastic cell transformation. Phosphorylated at Ser-29 (H3S28ph) by MLTK isoform 1, RPS6KA5 or AURKB during mitosis or upon ultraviolet B irradiation. Phosphorylation at Thr-7 (H3T6ph) by PRKCBB is a specific tag for epigenetic transcriptional activation that prevents demethylation of Lys-5 (H3K4me) by LSD1/KDM1A. At centromeres, specifically phosphorylated at Thr-12 (H3T11ph) from prophase to early anaphase, by DAPK3 and PKN1. Phosphorylation at Thr-12 (H3T11ph) by PKN1 is a specific tag for epigenetic transcriptional activation that promotes demethylation of Lys-10 (H3K9me) by KDM4C/JMJD2C. Phosphorylation at Tyr-42 (H3Y41ph) by JAK2 promotes exclusion of CBX5 (HP1 alpha) from chromatin.
    Monoubiquitinated by RAG1 in lymphoid cells, monoubiquitination is required for V(D)J recombination (By similarity). Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
  • 细胞定位

    Nucleus. Chromosome.
  • Target information above from: UniProt accession P68431 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 8350 Human
    • Entrez Gene: 8351 Human
    • Entrez Gene: 8352 Human
    • Entrez Gene: 8353 Human
    • Entrez Gene: 8354 Human
    • Entrez Gene: 8355 Human
    • Entrez Gene: 8356 Human
    • Entrez Gene: 8357 Human
    • Entrez Gene: 8358 Human
    • Entrez Gene: 8968 Human
    • Entrez Gene: 319152 Mouse
    • Entrez Gene: 319153 Mouse
    • Entrez Gene: 360198 Mouse
    • Entrez Gene: 97908 Mouse
    • Omim: 602810 Human
    • SwissProt: P68431 Human
    • SwissProt: P68433 Mouse
    • Unigene: 132854 Human
    • Unigene: 247813 Human
    • Unigene: 247814 Human
    • Unigene: 248176 Human
    • Unigene: 443021 Human
    • Unigene: 484990 Human
    • Unigene: 532144 Human
    • Unigene: 533292 Human
    • Unigene: 546315 Human
    • Unigene: 586261 Human
    • Unigene: 591778 Human
    • Unigene: 221301 Mouse
    • Unigene: 261657 Mouse
    • Unigene: 377874 Mouse
    • Unigene: 390558 Mouse
    • Unigene: 397328 Mouse
    see all
  • 别名

    • H3 histone family member E pseudogene antibody
    • H3 histone family, member A antibody
    • H3/A antibody
    • H31_HUMAN antibody
    • H3F3 antibody
    • H3FA antibody
    • Hist1h3a antibody
    • HIST1H3B antibody
    • HIST1H3C antibody
    • HIST1H3D antibody
    • HIST1H3E antibody
    • HIST1H3F antibody
    • HIST1H3G antibody
    • HIST1H3H antibody
    • HIST1H3I antibody
    • HIST1H3J antibody
    • HIST3H3 antibody
    • histone 1, H3a antibody
    • Histone cluster 1, H3a antibody
    • Histone H3 3 pseudogene antibody
    • Histone H3.1 antibody
    • Histone H3/a antibody
    • Histone H3/b antibody
    • Histone H3/c antibody
    • Histone H3/d antibody
    • Histone H3/f antibody
    • Histone H3/h antibody
    • Histone H3/i antibody
    • Histone H3/j antibody
    • Histone H3/k antibody
    • Histone H3/l antibody
    see all

图片

  • ChIP - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)
    ChIP - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)

    Chromatin was prepared from U2OS cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 min. The ChIP was performed with 25 µg of chromatin, 2 µg of ab4441 (blue), and 20 µl of protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach). Primers and probes are located in the first kb of the transcribed region.

  • ChIP - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)
    ChIP - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)This image is courtesy of an Abreview by Brian Segan.

    ChIP analysis using unpurified ab4441 binding Histon H3 in human primary CD34+ cell lysate. Cells were cross-linked for 15 minutes with 1% formaldehyde. Samples were incubated with primary antibody (0.2 µg/µg of chromatin) for 16 hours at 4°C. Protein binding was detected using real-time PCR.

    Positive controls:
    Region upstream of the transcription start site of the ACTB gene.
    Region upstream of the transcription start site of the EGR1 gene.

    Negative Controls:
    Region on chromosome 12 (Untr12) that is far from any known gene annotation and not expected to be bound by Histone H3 (acetyl K9).

    See Abreview

  • Western blot - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)
    Western blot - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)This image is courtesy of an anonymous Abreview.
    All lanes : Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441) at 1/2500 dilution

    Lane 1 : Mouse MEL cell nuclear lysate
    Lane 2 : Mouse MEL cell nuclear lysate treated with 0.4 µM Trichostatin A treatment for 18 hours.

    Lysates/proteins at 9 µg per lane.

    Secondary
    All lanes : Donkey Anti-Rabbit IgG H&L (HRP) (ab6802) at 1/20000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Exposure time: 1 minute

    See Abreview

  • Western blot - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)
    Western blot - Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441)
    All lanes : Anti-Histone H3 (acetyl K9) antibody - ChIP Grade (ab4441) at 0.5 µg/ml

    Lane 1 : Untreated HeLa cell acid-extract
    Lane 2 : HeLa cell acid-extract treated with sodium butyrate


    ab4441, Histone H3, acetylated (Lys9) Pab Rabbit x Human

实验方案

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (317)

    发表研究结果有使用 ab4441?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab4441 被引用在 317 文献中.

    • Dai L  et al. Resveratrol inhibits ACHN cells via regulation of histone acetylation. Pharm Biol 58:231-238 (2020). PubMed: 32202448
    • Hou T  et al. SIRT6 coordinates with CHD4 to promote chromatin relaxation and DNA repair. Nucleic Acids Res 48:2982-3000 (2020). PubMed: 31970415
    • Zhang T  et al. Histone H3K27 acetylation is dispensable for enhancer activity in mouse embryonic stem cells. Genome Biol 21:45 (2020). PubMed: 32085783
    • Ferrante F  et al. HDAC3 functions as a positive regulator in Notch signal transduction. Nucleic Acids Res 48:3496-3512 (2020). PubMed: 32107550
    • Payne JL  et al. Regulation of Small GTPase Rab20 by Ikaros in B-Cell Acute Lymphoblastic Leukemia. Int J Mol Sci 21:N/A (2020). PubMed: 32138279
    View all Publications for this product

    客户评价及客户问答

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    提交评价 提交问题

    1-10 of 46 Abreviews or Q&A

    Western blot abreview for Anti-Histone H3 (acetyl K9) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    15 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - nuclear (U2OS)
    Specification
    U2OS
    Blocking step
    Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    提交于 May 15 2014

    Western blot abreview for Anti-Histone H3 (acetyl K9) antibody - ChIP Grade

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    1 µg
    Gel Running Conditions
    Reduced Denaturing (18 % SDS-PAGE)
    Sample
    Dictyostelium discoideum Purified protein (Vegetative cells)
    Specification
    Vegetative cells
    Blocking step
    No blocking step used for 30 minute(s) · Concentration: 100% · Temperature: 25°C
    Read More

    Dr. Duen-Wei Hsu

    Verified customer

    提交于 Mar 31 2014

    Western blot abreview for Anti-Histone H3 (acetyl K9) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    10 µg
    Gel Running Conditions
    Reduced Denaturing (12)
    Sample
    Human Cell lysate - nuclear (Human HeLa cell)
    Specification
    Human HeLa cell
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    提交于 Nov 05 2013

    Western blot abreview for Anti-Histone H3 (acetyl K9) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    30 µg
    Gel Running Conditions
    Reduced Denaturing (12)
    Sample
    Mouse Cell lysate - whole cell (Mouse embryonic fibroblasts)
    Specification
    Mouse embryonic fibroblasts
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 24 2013

    Western blot abreview for Anti-Histone H3 (acetyl K9) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    3 µg
    Gel Running Conditions
    Reduced Denaturing (12)
    Sample
    Fruit fly (Drosophila melanogaster) Tissue lysate - nuclear (Fruit fly embryo)
    Specification
    Fruit fly embryo
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 21 2013

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Histone H3 (acetyl K9) antibody - ChIP Grade

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Blocking step
    Serum as blocking agent for 45 minute(s) · Concentration: 1.5% · Temperature: 25°C
    Antigen retrieval step
    Enzymatic
    Sample
    Rat Tissue sections (GONAD)
    Specification
    GONAD
    Permeabilization
    No
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 20 2013

    Western blot abreview for Anti-Histone H3 (acetyl K9) antibody - ChIP Grade

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    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (MEF)
    Gel Running Conditions
    Reduced Denaturing (12%)
    Loading amount
    10 µg
    Specification
    MEF
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

    Abcam user community

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    提交于 Jun 14 2019

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Histone H3 (acetyl K9) antibody - ChIP Grade

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    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Tammar wallaby Cell (ovary)
    Permeabilization
    Yes - 0.1% (v/v) Triton X-100 in PBS
    Specification
    ovary
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 27°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

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    提交于 Mar 13 2019

    Western blot abreview for Anti-Histone H3 (acetyl K9) antibody - ChIP Grade

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    Abreviews
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    abreview image
    Application
    Western blot
    Sample
    Schmidtea mediterranea Cell lysate - whole cell (Whole worm)
    Gel Running Conditions
    Reduced Denaturing (Invitrogen™ NuPAGE™ Novex™ 4-12% Bis-Tris Protein Gels, 1.0 mm, 10-well)
    Loading amount
    15 µg
    Specification
    Whole worm
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
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    提交于 Oct 22 2018

    Western blot abreview for Anti-Histone H3 (acetyl K9) antibody - ChIP Grade

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    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Saccharomyces cerevisiae Cell lysate - whole cell (MX4-22A strains (Morgan et al., 1991))
    Gel Running Conditions
    Reduced Denaturing (12.5% polyacrylamide)
    Loading amount
    4.5e+006 cells
    Specification
    MX4-22A strains (Morgan et al., 1991)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
    Read More

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