Application
Western blot
Sample
Mouse Cell lysate - whole cell (Primary ear fibroblast)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
20 µg
Specification
Primary ear fibroblast
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: TBST with 4% BSA
Dilution
1/3000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: anti- rabbit (santa cruz#sc-2004)
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
5 second(s)
Bands
Specific: 17 kDa
Additional data
Additional Notes
Lane 1: control
Lane 2: resveratrol treatment ( 25 um for 24 hrs)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 26 2019