Application
Western blot
Sample
Human Cell lysate - whole cell (liver)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
10 µg
Specification
liver
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: milk
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: GE healthcare
Host species: Rat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 17 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Sep 21 2018