Application
Western blot
Loading amount
9 µg
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Sample
Mouse Cell lysate - nuclear (MEL)
Specification
MEL
Treatment
0.4 ´M Trichostatin A treatment for 18 hr
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 1% skim milk
Dilution
1/2500
Detection
Detection method
ECL
Bands
Specific: 17 kDa
Exposure
1 minute(s) and 0 second(s)
Positive control
A - Untreated MEL cell
B - 0.4 ´M Trichostatin A treatment for 18 hr MEL cell
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提交于 Jan 30 2015