产品名称Anti-Histone H2B (yeast) (phospho T129)抗体
参阅全部 Histone H2B (yeast) 一抗
描述兔多克隆抗体to Histone H2B (yeast) (phospho T129)
经测试应用适用于: WBmore details
种属反应性与反应: Saccharomyces cerevisiae
Synthetic peptide corresponding to Saccharomyces cerevisiae Histone H2B (yeast) aa 100 to the C-terminus conjugated to Keyhole Limpet Haemocyanin (KLH).
(Peptide available as
- This antibody gave a positive signal in both Exponentially Growing Wild Type Yeast and Phleomycin Treated Yeast lysates.
存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Preservative: 0.02% Sodium azide
Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab92973 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 18 kDa (predicted molecular weight: 14 kDa).|
功能Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
序列相似性Belongs to the histone H2B family.
翻译后修饰Monoubiquitinated by the RAD6/UBC2-BRE1 complex to form H2BK123ub1. H2BK123ub1 gives a specific tag for epigenetic transcriptional activation and is also prerequisite for H3K4me and H3K79me formation. H2BK123ub1 also modulates the formation of double-strand breaks during meiosis and is a prerequisite for DNA-damage checkpoint activation. Deubiquitination is performed by UBP8 in presence of SGF11.
Phosphorylated by STE20 to form H2BS10ph during progression through meiotic prophase. May be correlated with chromosome condensation. H2BS10ph is also formed after H(2)O(2) treatment, and is a step leading to apoptosis.
Acetylated by GCN5, a component of the SAGA complex, to form H2BK11ac and H2BK16ac. H2BK16ac can also be formed by ESA1, a component of the NuA4 histone acetyltransferase (HAT) complex. Acetylation of N-terminal lysines and particularly formation of H2BK11acK16ac has a positive effect on transcription.
Sumoylation to form H2BK6su or H2BK7su, and probably also H2BK16su or H2BK17su, occurs preferentially near the telomeres and represses gene transcription.
- Information by UniProt
- H2B1_YEAST antibody
- Histone H2B.1 antibody
- Histone H2B.2 antibody
All lanes : Anti-Histone H2B (yeast) (phospho T129) antibody (ab92973) at 1 µg/ml
Lane 1 : Exponentially Growing WT Yeast Whole Cell Lysate
Lane 2 : Phleomycin Treated WT Yeast Whole Cell Lysate
Lane 3 : Exponentially Growing WT Yeast Whole Cell Lysate with Immunising peptide at 1 µg/ml
Lane 4 : Phleomycin Treated WT Yeast Whole Cell Lysate with Immunising peptide at 1 µg/ml
Lane 5 : Exponentially Growing WT Yeast Whole Cell Lysate with Control peptide at 1 µg/ml
Lane 6 : Phleomycin Treated WT Yeast Whole Cell Lysate with Control peptide at 1 µg/ml
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 14 kDa
Observed band size: 18 kDa why is the actual band size different from the predicted?
Additional bands at: 16 kDa, 160 kDa, 180 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 3 minutes
This blot was produced using a 10% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab92973 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.