Anti-Histone H2A.Z抗体- ChIP Grade (ab97966)
Key features and details
- Rabbit polyclonal to Histone H2A.Z - ChIP Grade
- Suitable for: ICC/IF, WB, IHC-P, ChIP, IP
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Histone H2A.Z抗体- ChIP Grade
参阅全部 Histone H2A.Z 一抗 -
描述
兔多克隆抗体to Histone H2A.Z - ChIP Grade -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, WB, IHC-P, ChIP, IPmore details -
种属反应性
与反应: Human
预测可用于: Schizosaccharomyces pombe -
免疫原
Synthetic peptide corresponding to Human Histone H2A.Z aa 65-128.
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阳性对照
- MOLT4 or Raji cell lysate. ICC/IF Hela cells IHC-P: Human normal colon FFPE tissue sections.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab97966于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use a concentration of 0.5 µg/ml.
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WB |
1/500 - 1/3000. Predicted molecular weight: 14 kDa.
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IHC-P |
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ChIP | (1) |
Use 2 µg for 25 µg of chromatin.
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IP |
1/500 - 1/1000.
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说明 |
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ICC/IF
Use a concentration of 0.5 µg/ml. |
WB
1/500 - 1/3000. Predicted molecular weight: 14 kDa. |
IHC-P
Use a concentration of 0.1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ChIP
Use 2 µg for 25 µg of chromatin. |
IP
1/500 - 1/1000. |
靶标
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功能
Variant histone H2A which replaces conventional H2A in a subset of nucleosomes. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. May be involved in the formation of constitutive heterochromatin. May be required for chromosome segregation during cell division. -
序列相似性
Belongs to the histone H2A family. -
翻译后修饰
Monoubiquitination of Lys-122 gives a specific tag for epigenetic transcriptional repression.
Acetylated on Lys-5, Lys-8 and Lys-12 during interphase. Acetylation disappears at mitosis.
Monomethylated on Lys-5 and Lys-8 by SETD6. SETD6 predominantly methylates Lys-8, lys-5 being a possible secondary site.
Not phosphorylated. -
细胞定位
Nucleus. Chromosome. - Information by UniProt
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数据库链接
- Entrez Gene: 3015 Human
- Omim: 142763 Human
- SwissProt: P0C0S5 Human
- Unigene: 119192 Human
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别名
- H2A histone family member Z antibody
- H2A.z antibody
- H2A/z antibody
see all
图片
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Chromatin was prepared from HeLa cells according to the Abcam X-ChIP protocol. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 25µg of chromatin, 2µg of ab97966 (blue), and 20µl of Protein A/G sepharose beads. No antibody was added to the beads control (yellow). The immunoprecipitated DNA was quantified by real time PCR (Taqman approach for active and inactive loci, Sybr green approach for heterochromatic loci). Primers and probes are located in the first kb of the transcribed region.
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All lanes : Anti-Histone H2A.Z antibody - ChIP Grade (ab97966) at 1/3000 dilution
Lane 1 : MOLT4 whole cell lysate
Lane 2 : Raji whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 14 kDa
12% SDS PAGE -
IHC image of ab97966 staining Histone H3 (phospho T45) in human colon formalin fixed paraffin embedded tissue sections*, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab97966, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
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ab97966 staining Histone H2A.Z in HeLa cells. The cells were fixed with 100% methanol (5min) and then blocked in 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated with ab97966 at 0.5μg/ml overnight at +4°C, followed by a further incubation at room temperature for 1h with an AlexaFluor®488 Goat anti-Rabbit secondary (ab150077) at 2 μg/ml (shown in green). AlexaFluor®350 WGA was used at a 1/200 dilution and incubated for 1h with the cells, to label plasma membranes (shown in blue). Nuclear DNA was labelled in red with 1.25 μM DRAQ5™ (ab108410), which was added to the secondary antibody mixture. A secondary only negative control is displayed, which indicates that the Histone H2A.Z staining observed is due to primary antibody specificity and not to unspecific binding of the secondary antibody to the cells.
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ab97966 immunoprecipitating Histone H2A.Z protein in HeLa whole cell lysate/extract. Lane 1: 50 μg HeLa whole cell lysate/extract. Lane 2: Control with 2 μg of preimmune rabbit IgG. Lane 3: Immunoprecipitation of Histone H2A.Z protein by 2 μg of ab97966. The immunoprecipitated Histone H2A.Z protein was detected with ab97966 diluted at 1:1000. Anti-rabbit IgG was used as a secondary antibody.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab97966 尚未被引用在任何文献中。