Anti-Histone H2A.Z抗体- ChIP Grade (ab97966)

Thank you very much for providing that information.

I am sorry that the antibody did not prove successful with your S. pombe samples. I do hope that you feel it was still worthwhile trying. As I mentioned in my previous email, please do go ahead and claim your free primary antibody (or the equivalent price of ab97966 off any product in our catalogue). As mentioned previously, along with the discount code could you also please quote "antibody unpublished, testing discount agreed with Karin" when placing your order. This means customer services know to speak to me once the order goes through so the discount will be added.

I hope this information has been of help. If I can be of any further assistance, please do let me know.

I'm sorry about that. It might be that the system won't let you submit a review for an unpiblished antibody. I was not aware of this.

If you wouldn't mind, could you just share the information with me by filling in the relevant form attached to this email? This should only take 5 minutes. Could you also let me know how you would rate the antibody out of 5.

This information would only become accessible to others if we republish this antibody in the future.

Once the form has been returned to me, please go ahead and use the discount code I provided for you. Along with the discount code could you please quote "antibody unpublished, testing discount agreed with Karin". This means customer services know to speak to me once the order goes through so the discount will be added.

I look forward to receiving your reply.

Thank you for contacting me.

I am sorry that the Anti-Histone H2A.Z antibody (ab97966) has now been removed from the catalogue. I am sorry but I was not aware of this change when I suggested you test it. Let me reassure you that the antibody has not been removed due to any quality issues (if this were the case anyone who had purchased the affected antibody would have been informed).

You should still be able to submit an Abreview for the product. This can be done by following the link below:

https://www.abcam.com/index.html?pageconfig=resource&rid=10332&viapagetrap=abreviews

And clicking on the "https://www.abcam.com/index.html?pageconfig=your_reviews&strAccessMode=public_create".

You will need to log in to your account in order to achieve this. If you have any problems submitting the review please do let me know. As soon as the Abreview has been submitted, the discount code can be used to claim your free primary antibody (or the equivalent price of ab97966 off any product).

I hope this information has been of help. If you have any further questions please do let me know.

I am glad you found the information provided of help.

All you need to do in order to participate in the testing discount scheme is to purchase ab97966 as normal. Once you have used with yourS. pombe samples, please share the results observed by submitting an Abreview,regardless of whether they are positive or negative. In the Abreview form, under the "Additional comments" section please quote the following code:

Discount code: xxxxxxxx


Once the Abreview has been submitted, the code becomes active and you can then use it to claim a free primary antibody or the equivalent price of ab97966 off any product in our catalogue. This free product must be claimed within a 4 month period (by the 31st of October 2012).

More information on the testing discount scheme can be found from the following link:

https://www.abcam.com/collaboratordiscount

I hope I have explained the process sufficiently well for you. If you have any further questions, please do not hesitate to ask.

Otherwise, I wish you all the best with your experiments.

Thank you for contacting us and sorry for the delay in getting back to you.

I have checked the sequence provided and compared it to the immunogen of all the anti-Histone H2A.Z antibodies we have, including the ones you suggested. Unfortunately, the ones suggested by you all show very low homology between the immunogen used to raise the antibody and the S. pombe protein sequence. I would therefore not expect any of them to be able to detect the S. pombe protein.

I have however found one antibody that may be worth trying. This is the rabbit polyclonal ab97966. This has been raised againsta synthetic peptide taken from within the residues 65-128 of human Histone H2A.Z. The sequence alignment of this peptide (highlighted in yellow) can be viewed in the attachment of this email. As you can see the homology is very high, 93%. I would therefore expect the antibody to be able to detect the S. pombe protein. However, as we have not tested this ourselves I would not be able to guarantee it.

If you would like to purchase ab97966 for use with S. pombe samplesI would be able to offer you a testing discount.This offer means that if you purchase the antibody as normal and test the antibody for itsability to detect the S. pombe proteinby performing a Western blot, then let us know of the results through an Abreview (no matter whether positive or negative) you would be eligible for a free primary antibody of your choice from our catalog (or the equivalent price of ab97966 off any item in the catalogue). More information on this offer can be found here:

https://www.abcam.com/collaboratordiscount

If you would be interested in participating in this scheme please do let me know as a discount code needs to be issued prior to the purchase of ab97966.

I hope this information has been of help. If you require any further information please do not hesitate to contact us again.