Application
Western blot
Sample
Human Cell lysate - whole cell (Colon Cancer)
Loading amount
40 µg
Specification
Colon Cancer
Treatment
1.7 mM hydroxyurea or 0.1 ug/mL daunorubicin
Blocking step
Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/500
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C
Secondary antibody
Name
Non-Abcam antibody was used: Alexa anti-rabbit 680 nm
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Monoclonal
Conjugation: Alexa Fluor® 680
Dilution
1/5000
Detection
Detection method
Odyssey
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 14 kDa Non-specific: 18 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Ms. Rachel Beckerman
Verified customer
提交于 Oct 24 2007