Application
ChIP
Sample
Mouse Cell lysate - whole cell (L (fibroblast) cell line)
Specification
L (fibroblast) cell line
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Real-time PCR
Positive control
none
Negative control
none
Other product details
Concentration
0.25 µl/µg chromatin
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: ChIP dilution buffer
Additional data
Abcam response
Although no positive and negative controls have been included within this review ab4174 has been intensively tested in-house and passed quality control tests to be classified as ChIP grade.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 27 2007