Application
ChIP
Sample
Mouse Cell lysate - nuclear (3T3 RIPA lysate)
Specification
3T3 RIPA lysate
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde 1 %, 10 min, 37oC
Duration of cross-linking step: 0 second(s)
Specification of the cross-linking agent: formaldehyde 1 %, 10 min, 37oC
Detection step
Real-time PCR
Positive control
H2A.Z containing nucleosome in promoter
Negative control
irrelevant region upstream of promoter / stretch of coding region
Other product details
Concentration
0.04 µg/µg chromatin
Incubation time
0 minute(s)
Additional data
Additional Notes
This antibody will not give signals that are as strong as those from other Abcam antibodies against modified and unmodified histones (e.g. H2B, acetylated H3, acetylated H4), but still significantly above background (Sigma rabbit IgG).
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Aug 15 2006