Application
ChIP
Sample
Arabidopsis thaliana Tissue lysate - whole (Influorescences and siliques)
Specification
Influorescences and siliques
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 20 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Duration of cross-linking step: 20 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Real-time PCR
Positive control
Pure extract of genomic DNA from tissue lysate
-PCR amplification at a known H2A.Z associated site, the Arabidopsis FLC gene, exon 1 (~247 bp amplification)(Pubmed ID: 17220196)
-Input (no sepharose beads added)
-PCR amplification at a known H2A.Z associated site, the Arabidopsis FLC gene, exon 1 (~247 bp amplification)(Pubmed ID: 17220196)
-Input (no sepharose beads added)
Negative control
-Rabbit polyclonal IgG antibody
-Water (blank) in PCR amplification
-Water (blank) in PCR amplification
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C
Additional data
Additional Notes
-Immunoprecipitations using a smaller volume of tissue lysate (of 300mg of tissue) versus a larger concentration of antibody appear to yield better results after PCR amplification.
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提交于 Nov 14 2011