Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
20 µg
Specification
HeLa
Treatment
H2A.Z shRNA
Gel Running Conditions
Reduced Denaturing (15)
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/2500
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: TBST+0.5%BSA
Secondary antibody
Name
Non-Abcam antibody was used: Anti-Rabbit IgG (Fc), AP Conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Polyclonal
Conjugation: Alkaline Phosphatase
Dilution
1/5000
Detection
Detection method
NBT/BCIP
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 15 kDa Non-specific: 18, 125 kDa
Positive control
control shRNA treated
Negative control
H2A.Z shRNA treated
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
提交于 Jul 26 2010