Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.5% TritonX100 in PBS
Other product details
Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti-Rat Cy3
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy3 ®
Dilution
1/200
Additional data
Additional Notes
Asynchronous HeLa were paraformaldehyde fixed, permeablized, immunofluorescently labeled and counterstained with DAPI. No nuclear staining signal was apparent indicating that the antibody does not appear to recognize its epitope under these conditions in these cells. Methanol-fixed samples were also tried and did not produce a nuclear signal.
Abcam response
Thank you for your feedback. We have also found that this antibody does not seem to be suitable for use in ICC/IF experiments (see application notes section). We hope this information might be useful for future users.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
提交于 Mar 29 2010