Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Pancreatic cancer)
Specification
Pancreatic cancer
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Dako Target Retreival Solution
Permeabilization
No
Blocking step
Serum as blocking agent for 15 minute(s) · Concentration: 100% · Temperature: 20°C
Other product details
Dilution
1/1000
Incubation time
45 minute(s) · Temperature: 20°C · Diluent: Antibody Diluent (Dako)
Secondary antibody
Name
Non-Abcam antibody was used: Anti-rabbit/mouse HRP polymer (pre-diluted, Dako)
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Polyclonal
Conjugation: HRP polymer
Additional data
Additional Notes
After the primary antibody incubation a rabbit anti-rat link (Dako) was used (1/400 for 30min at 20oC) before the anti-rabbit polymer HRP was applied.
Three H2A.Z antibodies were tested in parallel (AB80150, AB80153 and AB4462). The staining pattern was very similar in each case and all three could probably be used up to 1/5000 dilution in order to reduce background. Other possible suggestions would be a longer antigen retrieval and an overnight primary incubation at 4oC.
Three H2A.Z antibodies were tested in parallel (AB80150, AB80153 and AB4462). The staining pattern was very similar in each case and all three could probably be used up to 1/5000 dilution in order to reduce background. Other possible suggestions would be a longer antigen retrieval and an overnight primary incubation at 4oC.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Antibody Solutions
Verified customer
提交于 Mar 11 2010