Anti-Histone H2A-Bbd抗体(ab4175)
Key features and details
- Rabbit polyclonal to Histone H2A-Bbd
- Suitable for: ICC, WB
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Histone H2A-Bbd抗体 -
描述
兔多克隆抗体to Histone H2A-Bbd -
宿主
Rabbit -
特异性
Known to detect the recombinant form of human Histone H2A BBD. However, no reaction has yet been demonstrated against the endogenous protein in histone preparations. We believe that this is because of the low abundance of this variant in such preparations. -
经测试应用
适用于: ICC, WBmore details -
种属反应性
与反应: Human -
免疫原
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常规说明
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab4175 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| ICC | Use a concentration of 3 µg/ml. Staining may not be specific for H2A.Bbd (see image). | |
| WB | 1/500. Detects a band of approximately 12.5 kDa (predicted molecular weight: 12.5 kDa). As the WB shows, we can not find any reaction with any of the different histone extracts that we have used from different sources (calf, human, chicken). However, the antibody is very specific against the recombinant form of the protein. |
靶标
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功能
Atypical histone H2A which can replace conventional H2A in some nucleosomes and is associated with active transcription and mRNA processing. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. Nucleosomes containing this histone are less rigid and organize less DNA than canonical nucleosomes in vivo. They are enriched in actively transcribed genes and associate with the elongating form of RNA polymerase. They associate with spliceosome components and are required for mRNA splicing. May participate in spermatogenesis. -
组织特异性
Present in mature sperm. -
序列相似性
Belongs to the histone H2A family. -
结构域
The docking domain is responsible for the weaker heterodimerization with H2B. -
细胞定位
Nucleus. Chromosome. Associated with the active X chromosome and with autosomes, while it is absent from the inactive X chromosome and excluded from Barr bodies. - Information by UniProt
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数据库链接
- Entrez Gene: 474381 Human
- Entrez Gene: 474382 Human
- Entrez Gene: 83740 Human
- Omim: 300445 Human
- SwissProt: P0C5Y9 Human
- SwissProt: P0C5Z0 Human
- Unigene: 534498 Human
- Unigene: 632841 Human
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别名
- H2A Barr body deficient antibody
- H2A Barr body-deficient antibody
- H2A histone family member B antibody
see all
图片
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Western blot - Anti-Histone H2A-Bbd antibody (ab4175)Juan Ausio
H2A-Bbd (ab4175)
CE= chicken erythrocyte (10 and 2 micrograms) , HE= HeLa cells (10 micrograms) and 2A*= recombinant H2ABbd (the numbers underneath the lanes are the number of nanograms loaded in each lane).
The primary antibody dilution was 1:500 and the secondary was 1:3000.As the WB shows, we can not find any reaction with any of the different histone extracts that we have used from different sources (calf, human, chicken). However, the antibody is very specific against the recombinant form of the protein.
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Immunocytochemistry - Anti-Histone H2A-Bbd antibody (ab4175)This image is courtesy of Petra Hajkova, University of CambridgeMouse embryonic fibroblasts (MEFs) were fixed in 4%PFA, permeabilised in PBS 1%BSA 0.1%TX-100 and incubated with ab4175 (anti-H2A.Bbd) antibody o/n. The antibody appears to show nucleolar staining. The antibody gave the same staining pattern in different cell types. The dilution used was 3ug/ml.
实验方案
数据表及文件
文献 (3)
ab4175 被引用在 3 文献中.
- Wu BJ et al. Localization and expression of histone H2A variants during mouse oogenesis and preimplantation embryo development. Genet Mol Res 13:5929-39 (2014). PubMed: 25117351
- Nashun B et al. Changes in the nuclear deposition of histone H2A variants during pre-implantation development in mice. Development 137:3785-94 (2010). WB, ICC/IF ; Mouse . PubMed: 20943707
- Lee JH et al. Chromatin analysis of occluded genes. Hum Mol Genet 18:2567-74 (2009). ChIP ; Human . PubMed: 19380460
