Anti-Histone H1.3 + Histone H1.4抗体(ab61177)
Key features and details
- Rabbit polyclonal to Histone H1.3 + Histone H1.4
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Human
- Isotype: IgG
概述
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产品名称
Anti-Histone H1.3 + Histone H1.4抗体 -
描述
兔多克隆抗体to Histone H1.3 + Histone H1.4 -
宿主
Rabbit -
特异性
This antibody detects both Histone H1.3 and Histone H1.4.
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经测试应用
适用于: IHC-P, WB, ICC/IFmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide within Human Histone H1.3 aa 1-100. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
存储溶液
pH: 7
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol, 0.87% Sodium chloride
Without Mg2+ and Ca2+ -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab61177于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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IHC-P |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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WB | (1) |
1/500 - 1/1000.
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ICC/IF |
1/500 - 1/1000.
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说明 |
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IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
WB
1/500 - 1/1000. |
ICC/IF
1/500 - 1/1000. |
靶标
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细胞定位
Histone H1.3: Nucleus. Chromosome. Histone H1.4: Nucleus. Chromosome. -
数据库链接
- Entrez Gene: 3007 Human
- Entrez Gene: 3008 Human
- GenBank: NP_005320 Human
- Omim: 142210 Human
- Omim: 142220 Human
- SwissProt: P10412 Human
- SwissProt: P16402 Human
- Unigene: 136857 Human
see all
图片
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All lanes : Anti-Histone H1.3 + Histone H1.4 antibody (ab61177)
Lane 1 : HepG2 (human liver hepatocellular carcinoma cell line) whole cell lysate
Lane 2 : HepG2 whole cell lysate with blocking peptide -
IHC image of ab61177 staining Histone H1.3 + Histone H1.4 in human colon formalin fixed paraffin embedded tissue sections, performed on a Leica Bond. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab61177, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. No primary antibody was used in the negative control (shown on the inset).
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
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ab61177 at 1/500 - 1/1000 dilution staining Histone H1.3 + Histone H1.4 in HuvEc cells, treated with serum (20%, 15mins) by Immunofluorescence.
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Paraffin-embedded human colon carcinoma tissue stained for Histone H1.3 + Histone H1.4 with ab61177 at 1/50 dilution in immunohistochemical analysis.
The sample in the right-hand panel is incubated with the immunizing peptide.
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (11)
ab61177 被引用在 11 文献中.
- Xu Q et al. Vascular senescence in progeria: role of endothelial dysfunction. Eur Heart J Open 2:oeac047 (2022). PubMed: 36117952
- Jin R et al. LEAFY is a pioneer transcription factor and licenses cell reprogramming to floral fate. Nat Commun 12:626 (2021). PubMed: 33504790
- Zhang X et al. Amphiphysin I cleavage by asparagine endopeptidase leads to tau hyperphosphorylation and synaptic dysfunction. Elife 10:N/A (2021). PubMed: 34018922
- Puttaraksa K et al. Parvovirus B19V Nonstructural Protein NS1 Induces Double-Stranded Deoxyribonucleic Acid Autoantibodies and End-Organ Damage in Nonautoimmune Mice. J Infect Dis 219:1418-1429 (2019). PubMed: 30346568
- Borriello M et al. Pinocembrin Protects from AGE-Induced Cytotoxicity and Inhibits Non-Enzymatic Glycation in Human Insulin. Cells 8:N/A (2019). PubMed: 31035509