样品类型Tissue, Adherent cells, Suspension cells
Histone Extraction Kit (ab113476) is suitable for a quick isolation of total histone extracts from cells and tissue samples from mammals and other species.
The amount of starting material can be as low as 105 cells or 1 mg of tissue. For best results, the cell number should be >106 cells or the tissue amount should be >10 mg.
A total of 100 standard extractions (using 107 cells or 100 mg of tissue per extraction) can be performed with this kit. Yield of the total histone proteins can be up to 0.4 mg per 107 cells or per 100 mg of tissue. The yield may vary depending on the cell or tissue type.
Histone purification is most often performed before the analysis of the global levels within a sample of post-translational histone modifications in western blot.
Chromatin immunoprecipitation (ChIP) is the most common technique used for the analysis of histone modifications at specific sites within the genome. Use a chromatin extraction kit instead of a histone purification kit for the preparation of samples for ChIP.
存放说明Store at +4°C. Please refer to protocols.
组件 100 tests 10X Pre-Lysis Buffer 1 x 10ml Balance Buffer 1 x 8ml DTT Solution 1 x 20µl Lysis Buffer 1 x 20ml
Histones were purified from mouse liver cells using ab113476. Each lane was loaded with 15μg of purified histone.
Image courtesy of Kevin Dalgaard, MPI for Immunobiology & Epigenetics, Freiburg, DE
Histone extracts were prepared from MCF-7 cells using the ab113476 and acetyl histone H3-K9 was quantified.
This product has been referenced in:
- Nowacka-Woszuk J et al. Transgenerational effects of prenatal restricted diet on gene expression and histone modifications in the rat. PLoS One 13:e0193464 (2018). Read more (PubMed: 29474484) »
- Wang J et al. ATAC-Seq analysis reveals a widespread decrease of chromatin accessibility in age-related macular degeneration. Nat Commun 9:1364 (2018). Read more (PubMed: 29636475) »