Anti-HIPPI/IFT57抗体(ab5205)
Key features and details
- Rabbit polyclonal to HIPPI/IFT57
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG
概述
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产品名称
Anti-HIPPI/IFT57抗体
参阅全部 HIPPI/IFT57 一抗 -
描述
兔多克隆抗体to HIPPI/IFT57 -
宿主
Rabbit -
特异性
This antibody has been shown to detect 0.1 ug/lane of recombinant human HIPPI/IFT57. It also detects a band of the expected size when tested on a yeast lysate containing a HIPPI/IFT57 fusion protein.
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经测试应用
适用于: WB, ICC/IFmore details -
种属反应性
与反应: Mouse, Human, Recombinant fragment -
免疫原
Synthetic peptide corresponding to Human HIPPI/IFT57 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab46941) -
常规说明
This product was previously labelled as HIPPI
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading... -
纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
Our Abpromise guarantee covers the use of ab5205 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa. | |
| ICC/IF | Use a concentration of 5 µg/ml. |
靶标
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功能
Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear. -
组织特异性
Present in many tissues such as brain, thymus, lymph node, lung, liver, skin and kidney (at protein level). -
序列相似性
Belongs to the IFT57 family. -
结构域
The pseudo DED region (pDED) meadiates the interaction with HIP1. -
细胞定位
Cytoplasm > cytoskeleton > cilium basal body. Concentrates within the inner segment of cilia. - Information by UniProt
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数据库链接
- Entrez Gene: 55081 Human
- Entrez Gene: 73916 Mouse
- Omim: 606621 Human
- SwissProt: Q9NWB7 Human
- SwissProt: Q8BXG3 Mouse
- Unigene: 412196 Human
- Unigene: 241276 Mouse
- Unigene: 404371 Mouse
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别名
- CDNA FLJ10147 fis clone HEMBA1003369 antibody
- Dermal papilla derived protein 8 antibody
- Dermal papilla-derived protein 8 antibody
see all
图片
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Western blot - Anti-HIPPI/IFT57 antibody (ab5205)All lanes : Anti-HIPPI/IFT57 antibody (ab5205) at 1/1000 dilution
Lane 1 : Recombinant human HIPPI/IFT57 at 10 µg
Lane 2 : Recombinant human HIPPI/IFT57 at 1 µg
Lane 3 : Recombinant human HIPPI/IFT57 at 0.1 µg
Lane 4 : yeast extract + AD-HIPPI/IFT57 fusion protein
Lane 5 : yeast extract
Lane 6 : human lymphoblastoid extract at 10 µg
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 45 kDa why is the actual band size different from the predicted?Western blot using ab5205 at 1/1000 for 1hr in TBST.
The indicated band in lane 4 is higher than the recombinant human HIPPI/IFT57 as it is a fusion protein with a Activation Domain.
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Immunocytochemistry/ Immunofluorescence - Anti-HIPPI/IFT57 antibody (ab5205)ICC/IF image of ab5205 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab5205, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).
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Western blot - Anti-HIPPI/IFT57 antibody (ab5205)All lanes : Anti-HIPPI/IFT57 antibody (ab5205) at 1 µg/ml
Lane 1 : Human heart tissue lysate - total protein (ab29431)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
Lane 4 :A431 whole cell lysate (ab7909)
Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 6 :HEK293 whole cell lysate (ab7902)
Lane 7 : Human brain tissue lysate - total protein (ab29466)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 43,49 kDa why is the actual band size different from the predicted?
This antibody recognizes a band at approximately 49 kDa in tissue lysates, however in whole cell lysates the protein appears to be running at 43 kDa. In previous studies this 43 kDa band has been blocked with the immunizing peptide (data not shown). -
Western blot - Anti-HIPPI/IFT57 antibody (ab5205)All lanes : Anti-HIPPI/IFT57 antibody (ab5205) at 1 µg/ml
Lane 1 :NIH 3T3 whole cell lysate (ab7179)
Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution
Performed under reducing conditions.
Predicted band size: 49 kDa
Observed band size: 43,49 kDa why is the actual band size different from the predicted?
This antibody recognizes a band at approximately 49 kDa in tissue lysates, however in whole cell lysates the protein appears to be running at 43 kDa.
实验方案
数据表及文件
文献 (2)
ab5205 被引用在 2 文献中.
- Datta M & Bhattacharyya NP Regulation of RE1 Protein Silencing Transcription Factor (REST) Expression by HIP1 Protein Interactor (HIPPI). J Biol Chem 286:33759-69 (2011). ChIP, IP . PubMed: 21832040
- Banerjee M et al. Transcription regulation of caspase-1 by R393 of HIPPI and its molecular partner HIP-1. Nucleic Acids Res 38:878-92 (2010). WB ; Human . PubMed: 19934260
