为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Neuroscience Neurology process Neurodegenerative disease Huntington's disease

Anti-HIPPI/IFT57抗体(ab5205)

  • Datasheet
  • SDS
Reviews (5) Submit a question References (2)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Western blot - Anti-HIPPI/IFT57 antibody (ab5205)
  • Immunocytochemistry/ Immunofluorescence - Anti-HIPPI/IFT57 antibody (ab5205)
  • Western blot - Anti-HIPPI/IFT57 antibody (ab5205)
  • Western blot - Anti-HIPPI/IFT57 antibody (ab5205)

Key features and details

  • Rabbit polyclonal to HIPPI/IFT57
  • Suitable for: WB, ICC/IF
  • Reacts with: Mouse, Human, Recombinant fragment
  • Isotype: IgG

你可能会感兴趣

二抗
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

查看更多相关产品

概述

  • 产品名称

    Anti-HIPPI/IFT57抗体
    参阅全部 HIPPI/IFT57 一抗
  • 描述

    兔多克隆抗体to HIPPI/IFT57
  • 宿主

    Rabbit
  • 特异性

    This antibody has been shown to detect 0.1 ug/lane of recombinant human HIPPI/IFT57. It also detects a band of the expected size when tested on a yeast lysate containing a HIPPI/IFT57 fusion protein.

  • 经测试应用

    适用于: WB, ICC/IFmore details
  • 种属反应性

    与反应: Mouse, Human, Recombinant fragment
  • 免疫原

    Synthetic peptide corresponding to Human HIPPI/IFT57 aa 50-150 conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab46941)

  • 常规说明

     This product was previously labelled as HIPPI

     

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS

    Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • 纯度

    Immunogen affinity purified
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Neuroscience
    • Neurology process
    • Neurodegenerative disease
    • Huntington's disease

相关产品

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab5205于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
WB (2)
Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa.
ICC/IF
Use a concentration of 5 µg/ml.
说明
WB
Use a concentration of 1 µg/ml. Predicted molecular weight: 49 kDa.
ICC/IF
Use a concentration of 5 µg/ml.

靶标

  • 功能

    Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway (By similarity). Has pro-apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear.
  • 组织特异性

    Present in many tissues such as brain, thymus, lymph node, lung, liver, skin and kidney (at protein level).
  • 序列相似性

    Belongs to the IFT57 family.
  • 结构域

    The pseudo DED region (pDED) meadiates the interaction with HIP1.
  • 细胞定位

    Cytoplasm > cytoskeleton > cilium basal body. Concentrates within the inner segment of cilia.
  • Target information above from: UniProt accession Q9NWB7 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 55081 Human
    • Entrez Gene: 73916 Mouse
    • Omim: 606621 Human
    • SwissProt: Q9NWB7 Human
    • SwissProt: Q8BXG3 Mouse
    • Unigene: 412196 Human
    • Unigene: 241276 Mouse
    • Unigene: 404371 Mouse
    • 别名

      • CDNA FLJ10147 fis clone HEMBA1003369 antibody
      • Dermal papilla derived protein 8 antibody
      • Dermal papilla-derived protein 8 antibody
      • DERP 8 antibody
      • DERP8 antibody
      • Esrrbl 1 antibody
      • Esrrbl1 antibody
      • ESRRBL1 protein antibody
      • Estrogen related receptor beta like 1 antibody
      • Estrogen related receptor beta like protein 1 antibody
      • Estrogen-related receptor beta-like protein 1 antibody
      • FLJ10147 antibody
      • Hip1 Interacting protein antibody
      • HIP1 interacting protein HIPPI antibody
      • Hip1 protein interactor antibody
      • HIP1-interacting protein antibody
      • HIPPI antibody
      • Huntingtin interacting protein 1 interacting protein antibody
      • Huntingtin interacting protein 1 protein interactor antibody
      • IFT 57 antibody
      • ift57 antibody
      • IFT57_HUMAN antibody
      • Intraflagellar transport 57 homolog (Chlamydomonas) antibody
      • Intraflagellar transport 57 homolog antibody
      • Intraflagellar transport protein 57 homolog antibody
      • MHS4R2 antibody
      • Vestrogen related receptor beta like 1 antibody
      see all

    图片

    • Western blot - Anti-HIPPI/IFT57 antibody (ab5205)
      Western blot - Anti-HIPPI/IFT57 antibody (ab5205)
      All lanes : Anti-HIPPI/IFT57 antibody (ab5205) at 1/1000 dilution

      Lane 1 : Recombinant human HIPPI/IFT57 at 10 µg
      Lane 2 : Recombinant human HIPPI/IFT57 at 1 µg
      Lane 3 : Recombinant human HIPPI/IFT57 at 0.1 µg
      Lane 4 : yeast extract + AD-HIPPI/IFT57 fusion protein
      Lane 5 : yeast extract

      Lane 6 : human lymphoblastoid extract at 10 µg

      Performed under reducing conditions.

      Predicted band size: 49 kDa
      Observed band size: 45 kDa why is the actual band size different from the predicted?



      Western blot using ab5205 at 1/1000 for 1hr in TBST.

      The indicated band in lane 4 is higher than the recombinant human HIPPI/IFT57 as it is a fusion protein with a Activation Domain.

    • Immunocytochemistry/ Immunofluorescence - Anti-HIPPI/IFT57 antibody (ab5205)
      Immunocytochemistry/ Immunofluorescence - Anti-HIPPI/IFT57 antibody (ab5205)

      ICC/IF image of ab5205 stained human HeLa cells. The cells were methanol fixed (5 min) and incubated with the antibody (ab5205, 1µg/ml) for 1h at room temperature. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Image-iTTM FX Signal Enhancer was used as the primary blocking agent, 5% BSA (in TBS-T) was used for all other blocking steps. DAPI was used to stain the cell nuclei (blue). Alexa Fluor® 594 WGA was used to label plasma membranes (red).

    • Western blot - Anti-HIPPI/IFT57 antibody (ab5205)
      Western blot - Anti-HIPPI/IFT57 antibody (ab5205)
      All lanes : Anti-HIPPI/IFT57 antibody (ab5205) at 1 µg/ml

      Lane 1 : Human heart tissue lysate - total protein (ab29431)
      Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 3 : HeLa (Human epithelial carcinoma cell line) Nuclear Lysate
      Lane 4 : A-431 whole cell lysate (ab7909)
      Lane 5 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
      Lane 6 : HEK-293 whole cell lysate (ab7902)
      Lane 7 : Human brain tissue lysate - total protein (ab29466)

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 49 kDa
      Observed band size: 43,49 kDa why is the actual band size different from the predicted?



      This antibody recognizes a band at approximately 49 kDa in tissue lysates, however in whole cell lysates the protein appears to be running at 43 kDa. In previous studies this 43 kDa band has been blocked with the immunizing peptide (data not shown).
    • Western blot - Anti-HIPPI/IFT57 antibody (ab5205)
      Western blot - Anti-HIPPI/IFT57 antibody (ab5205)
      All lanes : Anti-HIPPI/IFT57 antibody (ab5205) at 1 µg/ml

      Lane 1 : NIH/3T3 whole cell lysate (ab7179)
      Lane 2 : MEF1 (Mouse embryonic fibroblast cell line) Whole Cell Lysate
      Lane 3 : Brain (Mouse) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : IRDye 680 Conjugated Goat Anti-Rabbit IgG (H+L) at 1/10000 dilution

      Performed under reducing conditions.

      Predicted band size: 49 kDa
      Observed band size: 43,49 kDa why is the actual band size different from the predicted?



      This antibody recognizes a band at approximately 49 kDa in tissue lysates, however in whole cell lysates the protein appears to be running at 43 kDa.

    实验方案

    • Western blot protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    数据表及文件

    • SDS download

    • Datasheet download

      Download

    文献 (2)

    发表研究结果有使用 ab5205?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab5205 被引用在 2 文献中.

    • Datta M & Bhattacharyya NP Regulation of RE1 Protein Silencing Transcription Factor (REST) Expression by HIP1 Protein Interactor (HIPPI). J Biol Chem 286:33759-69 (2011). ChIP, IP . PubMed: 21832040
    • Banerjee M  et al. Transcription regulation of caspase-1 by R393 of HIPPI and its molecular partner HIP-1. Nucleic Acids Res 38:878-92 (2010). WB ; Human . PubMed: 19934260

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-5 of 5 Abreviews or Q&A

    Western blot abreview for Anti-HIPPI/IFT57 antibody

    Average
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HCT116)
    Gel Running Conditions
    Reduced Denaturing (Gradient Gel)
    Loading amount
    100 µg
    Specification
    HCT116
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: RT°C
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 18 2014

    Immunoprecipitation abreview for Anti-HIPPI/IFT57 antibody

    Average
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Human Tissue lysate - nuclear (HCT116)
    Total protein in input
    100 µg
    Immuno-precipitation step
    Protein A/G
    Specification
    HCT116
    Read More

    Abcam user community

    Verified customer

    提交于 Feb 18 2014

    ChIP abreview for Anti-HIPPI antibody

    Average
    Abreviews
    Abreviews
    Application
    ChIP
    Sample
    Human Cell lysate - nuclear (Hela Cells)
    Specification
    Hela Cells
    Type
    Cross-linking (X-ChIP)
    Duration of cross-linking step: 15 minute(s) and 0 second(s)
    Specification of the cross-linking agent: 1% Formaldehyde
    Detection step
    Real-time PCR
    Negative control
    No DNA control
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 24 2012

    Immunoprecipitation abreview for Anti-HIPPI/IFT57 antibody

    Average
    Abreviews
    Abreviews
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (Hela Cells)
    Total protein in input
    5000 µg
    Immuno-precipitation step
    Protein G
    Specification
    Hela Cells
    Read More

    Dr. jerry fl

    Verified customer

    提交于 Jul 05 2012

    Western blot abreview for Anti-HIPPI antibody

    Good
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Hela Cells)
    Loading amount
    50 µg
    Specification
    Hela Cells
    Gel Running Conditions
    Reduced Denaturing (8%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jul 03 2012

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    沪ICP备14046373号-1

    沪公网安备 31011502007413号

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2021 艾博抗(上海)贸易有限公司版权所有