Anti-HIP55抗体(ab2836)
Key features and details
- Goat polyclonal to HIP55
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-HIP55抗体
参阅全部 HIP55 一抗 -
描述
山羊多克隆抗体to HIP55 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Dog
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免疫原
Synthetic peptide:
AANLSRNGPALQE
, corresponding to N terminal amino acids 2-14 of Human HIP55. -
常规说明
GenBank Accession Number – NP_054782.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab2836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 49 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Actin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin. -
序列相似性
Belongs to the ABP1 family.
Contains 1 ADF-H domain.
Contains 1 SH3 domain. -
结构域
The SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1. -
翻译后修饰
Degraded by caspases during apoptosis. -
细胞定位
Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cortical cytoskeleton. Associates with lamellipodial actin. - Information by UniProt
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数据库链接
- Entrez Gene: 28988 Human
- Entrez Gene: 13169 Mouse
- Entrez Gene: 83527 Rat
- Omim: 610106 Human
- SwissProt: Q9UJU6 Human
- SwissProt: Q62418 Mouse
- SwissProt: Q9JHL4 Rat
- Unigene: 436500 Human
see all -
别名
- ABP1 antibody
- Actin-binding protein 1 antibody
- Cervical mucin associated protein antibody
see all
图片
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Western blot - Anti-HIP55 antibody (ab2836)Anti-HIP55 antibody (ab2836) at 1 µg/ml + 3T3 cell lysate at 35 µg
Predicted band size: 49 kDa -
Western blot - Anti-HIP55 antibody (ab2836)ab2836 staining (1µ g/ml) of Jurkat lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
实验方案
数据表及文件
文献 (5)
ab2836 被引用在 5 文献中.
- Okur E & Yerlikaya A A novel and effective inhibitor combination involving bortezomib and OTSSP167 for breast cancer cells in light of label-free proteomic analysis. Cell Biol Toxicol N/A:N/A (2018). PubMed: 29948483
- Lenos KJ et al. Stem cell functionality is microenvironmentally defined during tumour expansion and therapy response in colon cancer. Nat Cell Biol 20:1193-1202 (2018). PubMed: 30177776
- Santos MS et al. Protein interactions of the vesicular glutamate transporter VGLUT1. PLoS One 9:e109824 (2014). PubMed: 25334008
- Hepper I et al. The mammalian actin-binding protein 1 is critical for spreading and intraluminal crawling of neutrophils under flow conditions. J Immunol 188:4590-601 (2012). ICC/IF ; Human . PubMed: 22450813
- Skelton TS et al. Downregulation of RhoA and changes in T cell cytoskeleton correlate with the abrogation of allograft rejection. Transpl Immunol 23:185-93 (2010). IHC-Fr ; Rat . PubMed: 20619345
