Anti-HIP55抗体(ab2836)
Key features and details
- Goat polyclonal to HIP55
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
概述
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产品名称
Anti-HIP55抗体
参阅全部 HIP55 一抗 -
描述
山羊多克隆抗体to HIP55 -
宿主
Goat -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Mouse, Human
预测可用于: Rat, Dog
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免疫原
Synthetic peptide:
AANLSRNGPALQE
, corresponding to N terminal amino acids 2-14 of Human HIP55. -
常规说明
GenBank Accession Number – NP_054782.Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA -
Concentration information loading... -
纯度
Immunogen affinity purified -
纯化说明
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
应用
Our Abpromise guarantee covers the use of ab2836 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | Use a concentration of 0.03 - 0.1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 49 kDa). 1 hour primary incubation is recommended for this product. |
靶标
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功能
Actin-binding adapter protein. May act as a common effector of antigen receptor-signaling pathways in leukocytes. Its association with dynamin suggests that it may also connect the actin cytoskeleton to endocytic function. Acts as a key component of the immunological synapse that regulates T-cell activation by bridging TCRs and the actin cytoskeleton to gene activation and endocytic processes. Binds to F-actin but is not involved in actin polymerization, capping or bundling. Does not bind G-actin. -
序列相似性
Belongs to the ABP1 family.
Contains 1 ADF-H domain.
Contains 1 SH3 domain. -
结构域
The SH3 domain mediates interaction with SHANK2, SHANK3 and PRAM1. -
翻译后修饰
Degraded by caspases during apoptosis. -
细胞定位
Cytoplasm > cytoskeleton. Cell projection > lamellipodium. Cortical cytoskeleton. Associates with lamellipodial actin. - Information by UniProt
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数据库链接
- Entrez Gene: 28988 Human
- Entrez Gene: 13169 Mouse
- Entrez Gene: 83527 Rat
- Omim: 610106 Human
- SwissProt: Q9UJU6 Human
- SwissProt: Q62418 Mouse
- SwissProt: Q9JHL4 Rat
- Unigene: 436500 Human
see all -
别名
- ABP1 antibody
- Actin-binding protein 1 antibody
- Cervical mucin associated protein antibody
see all
图片
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Western blot - Anti-HIP55 antibody (ab2836)Anti-HIP55 antibody (ab2836) at 1 µg/ml + 3T3 cell lysate at 35 µg
Predicted band size: 49 kDa -
Western blot - Anti-HIP55 antibody (ab2836)ab2836 staining (1µ g/ml) of Jurkat lysate (RIPA buffer, 35µ g total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.
实验方案
数据表及文件
文献 (5)
ab2836 被引用在 5 文献中.
- Okur E & Yerlikaya A A novel and effective inhibitor combination involving bortezomib and OTSSP167 for breast cancer cells in light of label-free proteomic analysis. Cell Biol Toxicol N/A:N/A (2018). PubMed: 29948483
- Lenos KJ et al. Stem cell functionality is microenvironmentally defined during tumour expansion and therapy response in colon cancer. Nat Cell Biol 20:1193-1202 (2018). PubMed: 30177776
- Santos MS et al. Protein interactions of the vesicular glutamate transporter VGLUT1. PLoS One 9:e109824 (2014). PubMed: 25334008
- Hepper I et al. The mammalian actin-binding protein 1 is critical for spreading and intraluminal crawling of neutrophils under flow conditions. J Immunol 188:4590-601 (2012). ICC/IF ; Human . PubMed: 22450813
- Skelton TS et al. Downregulation of RhoA and changes in T cell cytoskeleton correlate with the abrogation of allograft rejection. Transpl Immunol 23:185-93 (2010). IHC-Fr ; Rat . PubMed: 20619345
