Anti-HIF3 alpha/IPAS抗体(ab2165)

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Western blot - Anti-HIF3 alpha/IPAS antibody (ab2165)

    Key features and details

    • Rabbit polyclonal to HIF3 alpha/IPAS
    • Suitable for: WB, IHC-P
    • Reacts with: Mouse
    • Isotype: IgG

    概述

    • 产品名称

      Anti-HIF3 alpha/IPAS抗体
      参阅全部 HIF3 alpha/IPAS 一抗

    • 描述

      兔多克隆抗体to HIF3 alpha/IPAS

    • 宿主

      Rabbit

    • 经测试应用

      适用于: WB, IHC-Pmore details

    • 种属反应性

      与反应: Mouse
      预测可用于: Rat

    • 免疫原

      Synthetic peptide corresponding to Rabbit HIF3 alpha/IPAS aa 581-592 conjugated to keyhole limpet haemocyanin.
      Sequence:

      EIKPPKRSPRLE

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab2165于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/1600. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
    IHC-P
    Use at an assay dependent concentration. PubMed: 23451260
    说明
    WB
    1/1600. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
    IHC-P
    Use at an assay dependent concentration. PubMed: 23451260

    靶标

    • 功能

      Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea.

    • 组织特异性

      Expressed in kidney. Expressed abundantly in lung epithelial cells. Expression is regulated in an oxygen-dependent manner.

    • 序列相似性

      Contains 1 basic helix-loop-helix (bHLH) domain.
      Contains 2 PAS (PER-ARNT-SIM) domains.

    • 翻译后修饰

      In normoxia, hydroxylated on Pro-492 in the oxygen-dependent degradation domain (ODD) by PHD. The hydroxylated proline promotes interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation.

    • 细胞定位

      Nucleus. Cytoplasm. In the nuclei of all periportal and perivenous hepatocytes. In the distal perivenous zone, detected in the cytoplasm of the hepatocytes.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Basic-helix-loop-helix-PAS protein MOP7 antibody
        • bHLHe17 antibody
        • Class E basic helix-loop-helix protein 17 antibody
        • HIF 3A antibody
        • HIF 3A4 antibody
        • HIF-3-alpha antibody
        • HIF3 alpha antibody
        • HIF3-alpha antibody
        • HIF3-alpha-1 antibody
        • HIF3A antibody
        • HIF3A_HUMAN antibody
        • Hypoxia Inducible Factor 3 alpha antibody
        • Hypoxia inducible factor 3 alpha subunit antibody
        • Hypoxia inducible factor three alpha antibody
        • Hypoxia-inducible factor 3-alpha antibody
        • Inhibitory PAS domain protein antibody
        • IPAS antibody
        • Member of PAS protein 7 antibody
        • MOP7 antibody
        • PAS domain-containing protein 7 antibody
        • PASD7 antibody
        see all

      图片

      • Western blot - Anti-HIF3 alpha/IPAS antibody (ab2165)
        Western blot - Anti-HIF3 alpha/IPAS antibody (ab2165)
        Anti-HIF3 alpha/IPAS antibody (ab2165) at 1/1600 dilution + 3T3 lysate treated with Cobalt (II) chloride at 10 µg/ml

        Secondary
        Goat-anti-Rabbit at 1/10000 dilution

        Predicted band size: 72 kDa
        Observed band size: 72 kDa
        Additional bands at: 24 kDa, 55 kDa. We are unsure as to the identity of these extra bands.



        Exponentially growing 3T3 cells were treated with 130µM CoCl2 for 6 hours at 37°C to mimic hypoxia. A clear band at ~ 72kDa is detected in this lysate although some weaker background staining is also seen.

      文献 (3)

      发表研究结果有使用 ab2165？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab2165 被引用在 3 文献中.

      • Wang Y  et al. Berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3a. Biosci Rep 39:N/A (2019). PubMed: 31652442
      • Huang Y  et al. Hypoxia inducible factor 3a plays a critical role in alveolarization and distal epithelial cell differentiation during mouse lung development. PLoS One 8:e57695 (2013). IHC-P ; Mouse . PubMed: 23451260
      • Huang C & Hales BF Effects of exposure to a DNA damaging agent on the hypoxia inducible factors in organogenesis stage mouse limbs. PLoS One 7:e51937 (2012). WB ; Mouse . PubMed: 23251655

      客户评价及客户问答

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      Question

      Thanks for the antibody info! I think we will order it. I have one question: which isoform(s) does this antibody react with? Thanks!

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      Answer

      The antibody recognises Hif-3 alpha. The sequence of the immunogen is EIKPPKRSPRLE conjugated to KLH, corresponding to amino acids 581 - 592 of Mouse HIF-3 alpha.

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