Anti-HIF3 alpha/IPAS抗体(ab2165)

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Western blot - Anti-HIF3 alpha/IPAS antibody (ab2165)

    Key features and details

    • Rabbit polyclonal to HIF3 alpha/IPAS
    • Suitable for: WB
    • Reacts with: Mouse
    • Isotype: IgG

    概述

    • 产品名称

      Anti-HIF3 alpha/IPAS抗体
      参阅全部 HIF3 alpha/IPAS 一抗

    • 描述

      兔多克隆抗体to HIF3 alpha/IPAS

    • 宿主

      Rabbit

    • Tested Applications & Species

      Application Species
      WB
      Mouse
      See all applications and species data

    • 免疫原

      Synthetic peptide corresponding to Rabbit HIF3 alpha/IPAS aa 581-592 conjugated to keyhole limpet haemocyanin.
      Sequence:

      EIKPPKRSPRLE

      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 常规说明

      Previously labelled as HIF3 alpha. 

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

      One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

      Learn more here.

    性能

    应用

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab2165 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    应用 Species
    WB
    Mouse
    All applications
    Rat
    应用 Ab评论 说明
    WB
    1/1600. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
    说明
    WB
    1/1600. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).

    靶标

    • 功能

      Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea.

    • 组织特异性

      Expressed in kidney. Expressed abundantly in lung epithelial cells. Expression is regulated in an oxygen-dependent manner.

    • 序列相似性

      Contains 1 basic helix-loop-helix (bHLH) domain.
      Contains 2 PAS (PER-ARNT-SIM) domains.

    • 翻译后修饰

      In normoxia, hydroxylated on Pro-492 in the oxygen-dependent degradation domain (ODD) by PHD. The hydroxylated proline promotes interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation.

    • 细胞定位

      Nucleus. Cytoplasm. In the nuclei of all periportal and perivenous hepatocytes. In the distal perivenous zone, detected in the cytoplasm of the hepatocytes.
    • Information by UniProt

    • 数据库链接

      • 别名

        • Basic-helix-loop-helix-PAS protein MOP7 antibody
        • bHLHe17 antibody
        • Class E basic helix-loop-helix protein 17 antibody
        • HIF 3A antibody
        • HIF 3A4 antibody
        • HIF-3-alpha antibody
        • HIF3 alpha antibody
        • HIF3-alpha antibody
        • HIF3-alpha-1 antibody
        • HIF3A antibody
        • HIF3A_HUMAN antibody
        • Hypoxia Inducible Factor 3 alpha antibody
        • Hypoxia inducible factor 3 alpha subunit antibody
        • Hypoxia inducible factor three alpha antibody
        • Hypoxia-inducible factor 3-alpha antibody
        • Inhibitory PAS domain protein antibody
        • IPAS antibody
        • Member of PAS protein 7 antibody
        • MOP7 antibody
        • PAS domain-containing protein 7 antibody
        • PASD7 antibody
        see all

      图片

      • Western blot - Anti-HIF3 alpha/IPAS antibody (ab2165)
        Western blot - Anti-HIF3 alpha/IPAS antibody (ab2165)
        Anti-HIF3 alpha/IPAS antibody (ab2165) at 1/1600 dilution + 3T3 lysate treated with Cobalt (II) chloride at 10 µg/ml

        Secondary
        Goat-anti-Rabbit at 1/10000 dilution

        Predicted band size: 72 kDa
        Observed band size: 72 kDa
        Additional bands at: 24 kDa, 55 kDa. We are unsure as to the identity of these extra bands.



        Exponentially growing 3T3 cells were treated with 130µM CoCl2 for 6 hours at 37°C to mimic hypoxia. A clear band at ~ 72kDa is detected in this lysate although some weaker background staining is also seen.

      文献 (3)

      发表研究结果有使用 ab2165？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab2165 被引用在 3 文献中.

      • Wang Y  et al. Berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3a. Biosci Rep 39:N/A (2019). PubMed: 31652442
      • Huang Y  et al. Hypoxia inducible factor 3a plays a critical role in alveolarization and distal epithelial cell differentiation during mouse lung development. PLoS One 8:e57695 (2013). IHC-P ; Mouse . PubMed: 23451260
      • Huang C & Hales BF Effects of exposure to a DNA damaging agent on the hypoxia inducible factors in organogenesis stage mouse limbs. PLoS One 7:e51937 (2012). WB ; Mouse . PubMed: 23251655

      客户评价及客户问答

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      Question

      Thanks for the antibody info! I think we will order it. I have one question: which isoform(s) does this antibody react with? Thanks!

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      Answer

      The antibody recognises Hif-3 alpha. The sequence of the immunogen is EIKPPKRSPRLE conjugated to KLH, corresponding to amino acids 581 - 592 of Mouse HIF-3 alpha.

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