Anti-HIF3 alpha/IPAS抗体(ab2165)
Key features and details
- Rabbit polyclonal to HIF3 alpha/IPAS
- Suitable for: WB
- Reacts with: Mouse
- Isotype: IgG
概述
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产品名称
Anti-HIF3 alpha/IPAS抗体
参阅全部 HIF3 alpha/IPAS 一抗 -
描述
兔多克隆抗体to HIF3 alpha/IPAS -
宿主
Rabbit -
Tested Applications & Species
Application Species WB Mouse -
免疫原
Synthetic peptide corresponding to Rabbit HIF3 alpha/IPAS aa 581-592 conjugated to keyhole limpet haemocyanin.
Sequence:EIKPPKRSPRLE
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常规说明
Previously labelled as HIF3 alpha.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.01% Sodium azide
Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2165 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
应用 | Species |
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WB |
Mouse
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All applications |
Rat
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应用 | Ab评论 | 说明 |
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WB |
1/1600. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa).
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说明 |
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WB
1/1600. Detects a band of approximately 72 kDa (predicted molecular weight: 72 kDa). |
靶标
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功能
Involved in adaptive response to hypoxia. Suppresses hypoxia-inducible expression of HIF1A and EPAS1. Binds to core DNA sequence 5'-TACGTG-3' within the hypoxia response element (HRE) of target gene promoters. The complex HIF3A-ARNT activates the transcription of reporter genes driven by HRE. Isoform 4 has a dominant-negative function of inactivating HIF1A-mediated transcription. Isoform 4 attenuates the binding of HIF1A to hypoxia-responsive elements (HRE), thus inhibiting HRE-driven transcription. Hypoxia induces down-regulation of isoform 4, leading to activation of HIF1A in hypoxia. Conversely, upon restoring normoxia, the expression of isoform 4 increases and thereby secure an inhibition of HIF1A activity. Isoform 4 may be a negative regulator of hypoxia-inducible gene expression in the kidney and may be involved in renal tumorigenesis. Functions as an inhibitor of angiogenesis in the cornea. -
组织特异性
Expressed in kidney. Expressed abundantly in lung epithelial cells. Expression is regulated in an oxygen-dependent manner. -
序列相似性
Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
翻译后修饰
In normoxia, hydroxylated on Pro-492 in the oxygen-dependent degradation domain (ODD) by PHD. The hydroxylated proline promotes interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. -
细胞定位
Nucleus. Cytoplasm. In the nuclei of all periportal and perivenous hepatocytes. In the distal perivenous zone, detected in the cytoplasm of the hepatocytes. - Information by UniProt
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数据库链接
- Entrez Gene: 53417 Mouse
- Entrez Gene: 64345 Rat
- SwissProt: Q0VBL6 Mouse
- SwissProt: Q9JHS2 Rat
- Unigene: 135110 Mouse
- Unigene: 161814 Rat
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别名
- Basic-helix-loop-helix-PAS protein MOP7 antibody
- bHLHe17 antibody
- Class E basic helix-loop-helix protein 17 antibody
see all
图片
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Anti-HIF3 alpha/IPAS antibody (ab2165) at 1/1600 dilution + 3T3 lysate treated with Cobalt (II) chloride at 10 µg/ml
Secondary
Goat-anti-Rabbit at 1/10000 dilution
Predicted band size: 72 kDa
Observed band size: 72 kDa
Additional bands at: 24 kDa, 55 kDa. We are unsure as to the identity of these extra bands.Exponentially growing 3T3 cells were treated with 130µM CoCl2 for 6 hours at 37°C to mimic hypoxia. A clear band at ~ 72kDa is detected in this lysate although some weaker background staining is also seen.
数据表及文件
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SDS download
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Datasheet download
文献 (3)
ab2165 被引用在 3 文献中.
- Wang Y et al. Berberine improves insulin resistance in adipocyte models by regulating the methylation of hypoxia-inducible factor-3a. Biosci Rep 39:N/A (2019). PubMed: 31652442
- Huang Y et al. Hypoxia inducible factor 3a plays a critical role in alveolarization and distal epithelial cell differentiation during mouse lung development. PLoS One 8:e57695 (2013). IHC-P ; Mouse . PubMed: 23451260
- Huang C & Hales BF Effects of exposure to a DNA damaging agent on the hypoxia inducible factors in organogenesis stage mouse limbs. PLoS One 7:e51937 (2012). WB ; Mouse . PubMed: 23251655