为了使您在Abcam官网的浏览体验更顺畅,请使用最新版本的浏览器比如 Google Chrome

我们使用cookies,使我们网站尽可能对您有帮助。

我们的Cookie政策介绍了您如何能够取消cookie使用。

如果您不改变cookie设置,我们认为您愿意接受cookie。

继续 继续

致电 +86 21 2070 0500 或 联系我们

  • 我的账户
  • 退出
登录 或 注册

欢迎光临

登录 或

您还没有帐户?

注册
我的购物篮
快速订购
Abcam homepage

  • 研究用途的产品
    按产品类型
    一抗
    二抗
    免疫测定试剂盒与试剂
    细胞与组织成像工具
    细胞与生化测定
    蛋白质与肽
    按产品类型
    蛋白质组学工具
    激动剂、活化剂、拮抗剂与抑制剂
    细胞株和裂解液
    多重miRNA检测
    多因子分析试剂盒
    按研究领域
    癌症
    心血管
    细胞生物学
    表观遗传学
    代谢
    发育生物学
    按研究领域
    免疫学
    微生物学
    神经科学
    信号转导
    干细胞
  • 诊断和治疗的解决方案
    定制方案及合作关系

    定制抗体开发及商务合作助力您的诊断和治疗探索。

    与我们一起开创定制方案

    与我们成为伙伴

  • 技术支持
    支持中心

    获取研究建议和支持

    查看支持中心

    Protocols

    一步一步完成您的实验

    查看Protocols

  • 活动通知
    • 会议年历
    • 肿瘤研究
    • 心血管研究
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 神经生物学
    • 干细胞研究
    • 商品展览
    • 实验技术视频
    查看最新活动

    活动说明、摘要提交、演讲者、注册方式及更多信息

    查看全球活动安排

  • 通路
    细胞信号转导通路

    查看所有通路

    查看交叉通路

在新冠疫情期间支持我们的客户和员工–了解更多

Cardiovascular Hypoxia HIF

Anti-HIF1 beta抗体[2B10] (ab2771)

  • Datasheet
  • SDS
Reviews (7)Q&A (5)References (16)

Product price, shipping and contact information

Currently unavailable

抱歉,我们目前无法显示此内容。

请联系我们以完成下单,或者之后再尝试访问。

 

正在加载规格和价格。。。

 

送货和订单信息

Abpromise

产品质量保证,客户专家支持,

点击获取更多

Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)
  • Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)
  • Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [2B10] (ab2771)
  • Flow Cytometry - Anti-HIF1 beta antibody [2B10] (ab2771)

Key features and details

  • Mouse monoclonal [2B10] to HIF1 beta
  • Suitable for: Flow Cyt, ICC/IF, IHC-P
  • Reacts with: Mouse, Rat, Human, Xenopus laevis, Fish, Non human primates, Zebrafish
  • Isotype: IgG1

你可能会感兴趣

一抗
Product image
HRP Anti-6X His tag® antibody (ab1187)
蛋白
Product image
Recombinant Human HIF1 beta protein (ab157907)
二抗
Product image
Goat Anti-Mouse IgG H&L (HRP) (ab205719)

查看更多相关产品

概述

  • 产品名称

    Anti-HIF1 beta抗体[2B10]
    参阅全部 HIF1 beta 一抗
  • 描述

    小鼠单克隆抗体[2B10] to HIF1 beta
  • 宿主

    Mouse
  • 特异性

    Detects aryl hydrocarbon (Ah) receptor nuclear translocator (ARNT).
  • 经测试应用

    适用于: Flow Cyt, ICC/IF, IHC-Pmore details
  • 种属反应性

    与反应: Mouse, Rat, Human, Xenopus laevis, Fish, Non human primates, Zebrafish
    预测可用于: Rabbit, Cow
  • 免疫原

    Synthetic peptide corresponding to Human HIF1 beta aa 771-789.
    Sequence:

    NSYNNEEFPDLTMFPPFSE

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 99% PBS
  • Concentration information loading...
  • 纯度

    Affinity purified
  • 克隆

    单克隆
  • 克隆编号

    2B10
  • 同种型

    IgG1
  • 研究领域

    • Cardiovascular
    • Hypoxia
    • HIF
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HLH / Leucine Zipper
    • HLH
    • Epigenetics and Nuclear Signaling
    • Cardiovascular/Immune
    • Hypoxia
    • HIF
    • Cancer
    • Invasion/microenvironment
    • Hypoxia
    • HIF
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Nucleotide metabolism
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • Compatible Secondaries

    • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
    • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
    • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
  • Conjugation kits

    • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
    • APC Conjugation Kit - Lightning-Link® (ab201807)
  • Isotype control

    • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
    • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
  • Recombinant Protein

    • Recombinant Human HIF1 beta protein (ab157907)

应用

Our Abpromise guarantee covers the use of ab2771 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
Flow Cyt Use 1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

ICC/IF 1/1000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

靶标

  • 功能

    Required for activity of the Ah (dioxin) receptor. This protein is required for the ligand-binding subunit to translocate from the cytosol to the nucleus after ligand binding. The complex then initiates transcription of genes involved in the activation of PAH procarcinogens. The heterodimer with HIF1A or EPAS1/HIF2A functions as a transcriptional regulator of the adaptive response to hypoxia.
  • 序列相似性

    Contains 1 basic helix-loop-helix (bHLH) domain.
    Contains 1 PAC (PAS-associated C-terminal) domain.
    Contains 2 PAS (PER-ARNT-SIM) domains.
  • 细胞定位

    Nucleus.
  • Target information above from: UniProt accession P27540 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 281010 Cow
    • Entrez Gene: 405 Human
    • Entrez Gene: 11863 Mouse
    • Entrez Gene: 100008996 Rabbit
    • Entrez Gene: 25242 Rat
    • Entrez Gene: 64277 Zebrafish
    • Omim: 126110 Human
    • SwissProt: Q9BE97 Cow
    • SwissProt: P27540 Human
    • SwissProt: P53762 Mouse
    • SwissProt: O02748 Rabbit
    • SwissProt: P41739 Rat
    • Unigene: 632446 Human
    • Unigene: 250265 Mouse
    • Unigene: 10520 Rat
    see all
  • 别名

    • arnT antibody
    • ARNT protein antibody
    • ARNT_HUMAN antibody
    • Aryl hydrocarbon receptor nuclear translocator antibody
    • bHLHe2 antibody
    • Class E basic helix-loop-helix protein 2 antibody
    • Dioxin receptor antibody
    • Dioxin receptor nuclear translocator antibody
    • Drnt antibody
    • HIF 1 beta antibody
    • HIF 1beta antibody
    • HIF-1-beta antibody
    • HIF1-beta antibody
    • HIF1B antibody
    • HIF1beta antibody
    • Hypoxia Inducible Factor 1 antibody
    • Hypoxia inducible factor 1 beta antibody
    • Hypoxia-inducible factor 1-beta antibody
    • nuclear translocator antibody
    • Tango antibody
    see all

图片

  • Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)
    Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)

    Immunocytochemistry/Immunofluorescence analysis of HIF1 beta shows staining in A2058 cells. HIF1 beta (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2771 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)
    Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)

    Immunocytochemistry/Immunofluorescence analysis of HIF1 beta shows staining in HeLa cells. HIF1 beta (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2771 (1:20) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)
    Immunocytochemistry/ Immunofluorescence - Anti-HIF1 beta antibody [2B10] (ab2771)

    Immunocytochemistry/Immunofluorescence analysis of HIF1 beta shows staining in U251 cells. HIF1 beta (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with ab2771 (1:100) overnight at 4°C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody. Images were taken at 60X magnification.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [2B10] (ab2771)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HIF1 beta antibody [2B10] (ab2771)
    IHC image of ab2771 staining in human tonsil formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab2771, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Flow Cytometry - Anti-HIF1 beta antibody [2B10] (ab2771)
    Flow Cytometry - Anti-HIF1 beta antibody [2B10] (ab2771)
    Overlay histogram showing PC12 cells stained with ab2771 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab2771, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in PC12 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.

实验方案

  • Flow cytometry protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

    • Datasheet
    • SDS
  • 文献 (16)

    发表研究结果有使用 ab2771?请让我们知道,以便我们可以引用本数据表中的参考文章。

    ab2771 被引用在 16 文献中.

    • Wu CS  et al. ASC modulates HIF-1a stability and induces cell mobility in OSCC. Cell Death Dis 11:721 (2020). PubMed: 32883954
    • Fu YC & Xin ZM Inhibited corneal neovascularization in rabbits following corneal alkali burn by double-target interference for VEGF and HIF-1a. Biosci Rep 39:N/A (2019). PubMed: 30355648
    • Chen WC  et al. Aryl hydrocarbon receptor modulates stroke-induced astrogliosis and neurogenesis in the adult mouse brain. J Neuroinflammation 16:187 (2019). PubMed: 31606043
    • Madan E  et al. HIF-transcribed p53 chaperones HIF-1a. Nucleic Acids Res 47:10212-10234 (2019). PubMed: 31538203
    • Zhang B  et al. Moxibustion alleviates intervertebral disc degeneration via activation of the HIF-1a/VEGF pathway in a rat model. Am J Transl Res 11:6221-6231 (2019). PubMed: 31632589
    View all Publications for this product

    客户评价及客户问答

    Show All 评价 Q&A
    提交评价 提交问题

    1-10 of 12 Abreviews or Q&A

    Question

    Inquiry: I am just wondering if this antibody can react with mouse brain homogenates? Do you have any literature to show the result? Thanks a lot!

    Read More

    Abcam community

    Verified customer

    Asked on Jun 10 2013

    Answer

    While we do have publications using this antibody in mouse samples we are not presently aware of specific publications using mouse brain homogenates. HIF1 beta forms heterodimers with HIF1alpha and has ubiquitous nuclear expression. The HIF1 complex rapidly degrades. It is critical to prep only a few plates/dishes/flasks of cells at a time and to immediately get the cells into ice cold buffers and perform the whole protein prep on ice. HIF-1 is largely undetectable in cells or tissues grown under normoxic conditions. It is stabilized only at O2 concentrations below 5% or with treat using certain agents (CoCl2, DFO, etc.) so proper sample preparation is critical. Upon stabilization HIF-1 translocates to the nucleus. The best western blots (cleanest) are always done using nuclear extracts. It is possible to detect HIF-1 in whole cell extracts, but they tend to be much dirtier and the staining is much weaker. We recommend that a positive/negative control always be run side by side so that it is possible to discern which band is upregulated in the hypoxic sample. Depending on the sample, treatment, etc. you may see either a band or a doublet.

    Read More

    Abcam Scientific Support

    回复于 Jun 10 2013

    Western blot abreview for Anti-HIF1 beta antibody [2B10]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (HOS cells)
    Loading amount
    50 µg
    Specification
    HOS cells
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
    Read More

    Abcam user community

    Verified customer

    提交于 Mar 03 2009

    Immunoprecipitation abreview for Anti-HIF1 beta antibody [2B10]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunoprecipitation
    Sample
    Human Cell lysate - whole cell (293T)
    Total protein in input
    500 µg
    Specification
    293T
    Immuno-precipitation step
    Protein G
    Read More

    Abcam user community

    Verified customer

    提交于 Oct 30 2007

    Western blot abreview for Anti-HIF1 beta antibody [2B10]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    African Green Monkey Cell lysate - whole cell (Cos-7 cell line)
    Loading amount
    30 µg
    Specification
    Cos-7 cell line
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 26 2007

    Western blot abreview for Anti-HIF1 beta antibody [2B10]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Cell lysate - whole cell (3T3 cell line)
    Loading amount
    30 µg
    Specification
    3T3 cell line
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    提交于 Sep 26 2007

    Question

    BATCH NUMBER 271685 ORDER NUMBER -- NOT SPECIFIED -- DESCRIPTION OF THE PROBLEM Double bands, MW is around 90.For human and rat samples, the bands from homogenate are Darker than from extract opposite for mouse samples. The bands from mouse samples are much darker than the others. SAMPLE I tested the antibody with brain samples such as human homogenate, human extract, rat homogenate, rat extract, mouse homogenate and mouse extract. PRIMARY ANTIBODY HIF1 beta ab2771, 271685. 1:1000 diluted, in 5% non-fat milk incubate for 18 hours,overnight, room temperature wash with 0.1% tween-TBS for 3 times, at 10 minutes each time DETECTION METHOD ECL POSITIVE AND NEGATIVE CONTROLS USED not used ANTIBODY STORAGE CONDITIONS -20, but +4 degrees before use diluted (1:4) in 5%BSA. SAMPLE PREPARATION in SDS buffer containing 2%SDS, 10%glycerol, 1%beta-mercaptoethanol and 0.0625M Tris-Cl AMOUNT OF PROTEIN LOADED 10ug ELECTROPHORESIS/GEL CONDITIONS 10% reducing TRANSFER AND BLOCKING CONDITIONS buffer:1.4% glycine, 0.3% Trizma base, 20% methanol 1 hour block with 5% non-fat milk SECONDARY ANTIBODY goat anti-mouse 1:5000 diluted in 5% non-fat milk for 2 hours at room temperature wash with 0.1% tween-TBS for 3 times, at 10 minutes each time HOW MANY TIMES HAVE YOU TRIED THE APPLICATION? 2 HAVE YOU RUN A "NO PRIMARY" CONTROL? No DO YOU OBTAIN THE SAME RESULTS EVERY TIME? Yes WHAT STEPS HAVE YOU ALTERED? not altered ADDITIONAL NOTES I checked literature and found HIF1 beta has several isoforms. Does ab2771 recognize only one isoforms or all? I failed to attach the image of the film, which is jpg or doc format.

    Read More

    Abcam community

    Verified customer

    Asked on Jul 30 2007

    Answer

    Thank you for your patience. I am sorry to hear that you are experiencing difficulties with this product ab2771 in western blot. Often it is possible to make suggestions that help resolve problems. We will happily offer technical support and in the event that a product is not functioning in the applications cited on the product data sheet (and the problem has been reported within 120 days of purchase), and if it appears that the antibody is at fault, a replacement/credit note/refund will be offered. I have looked through the protocols you used and have a few questions and suggestions that might help you resolve the problem. In most cases, the cause of multiple bands is because the primary and/or secondary antibody concentration is too high and causes non-specific binding. You can try decreasing the primary (1:1000 ~ 1:5000) and secondary concentration or run a no-primary control (without the primary antibody) to ensure that the multiple bands are not caused by your secondary antibody. Another possible reason is that the extra bands you are observing may be multimers. Please try boiling your protein in SDS-PAGE for 10 minutes to disrupt multimers. Meanwhile, bands located below the expected molecular weight you are observing may indicate that your target protein has been digested. Please make sure that you incorporate sufficient protease inhibitors in your sample buffer. The image you provided showed different strengths in expression. I would suggest doing optimization for each species sample separately, so that you can have better control of the level and intensity of the bands. This is because your target protein may exist in different amount in different species. I would also suggest incubating your primary antibody at 4 degrees instead of at room temperature. Incubation at room temperature is recommended to be done for 1-2 hours, while 4 degrees can be done overnight. Unfortunately, I do not have any data suggesting that this product can detect other HIF-1 beta isoforms at this time. However, I believe the following reference may help explain the double band that you are observing. Rescue of Hypoxia-inducible Factor-1 alpha-deficient Tumor Growth by Wild-Type Cells Is Independent of Vascular Endothelial Growth Factor. Can. Res. 62(10):2962-2970, 2002. http://cancerres.aacrjournals.org/cgi/reprint/62/10/2962 In this specific reference, the authors detect nonspecific cross-reactivity of the anti-ARNT polyclonal antibody in nuclear extracts of ES cells. Also, we see similar reactivity in the reference: Isoform specific expression of hypoxia-inducible factor-1alpha during the late stages of mouse spermiogenesis. Mol. Endocrinol., Vol 16: 234, Feb 2002. http://mend.endojournals.org/cgi/reprint/16/2/234 I hope the above suggestions and references will be helpful to you. If you still experience problems please do not hesitate to contact me with details of your order (purchase order number, shipping address/purchasing agent info, contact information, etc.) so that I can immediately arrange for a replacement or refund to you. Also, please advice on how you would to proceed with this enquiry. I look forward to hearing from you again in order to resolve the matter.

    Read More

    Abcam Scientific Support

    回复于 Jul 30 2007

    Western blot abreview for Anti-HIF1 beta antibody [2B10]

    Average
    Abreviews
    Abreviews
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (forebrain)
    Loading amount
    15 µg
    Specification
    forebrain
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 15 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 22 2007

    Western blot abreview for Anti-HIF1 beta antibody [2B10]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Rat Cell lysate - whole cell (PC12)
    Loading amount
    20 µg
    Specification
    PC12
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 21 2007

    Western blot abreview for Anti-HIF1 beta antibody [2B10]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (U2OS)
    Loading amount
    20 µg
    Specification
    U2OS
    Gel Running Conditions
    Reduced Denaturing
    Blocking step
    Milk as blocking agent for 45 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    提交于 Jun 15 2007

    Question

    I have an inquiry about your product, ab2771; HIF1 beta antibody. Which cell line did you use this antibody for Immunoprecipitation? Was it transfected cells? The researcher wants to use this antibody for IP. And he found clone 2B10 except abcam wouldn't be recommended for IP. Please advise us if you have any infromation about this purpose.

    Read More

    Abcam community

    Verified customer

    Asked on Jan 06 2006

    Answer

    Thank you for your enquiry. Followingf corresponce with the source of this antibody I have determined that the cell lines used for IP were mouse Hep1c1c7 and human HeLa cells. ab2771 works well by IP in both mouse and human lysates at 1-2 ug/IP. I hope this information helps, please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    回复于 Jan 06 2006

    1-10 of 12 Abreviews or Q&A

    •  向后
    • 1
    • 2
    • 向前 

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
    For licensing inquiries, please contact partnerships@abcam.com

    通过邮件收到学术资源和促销信息 注册
    研究类别(A-Z)
    • 肿瘤研究
    • 心血管研究
    • 细胞生物学
    • 发育生物学
    • 染色质及细胞核信号转导研究
    • 免疫学
    • 代谢
    • 微生物学
    • 神经生物学
    • 信号转导研究
    • 干细胞研究
    产品种类(A-Z)
    • 一抗
    • 二抗
    • 生化试剂
    • 亚类对照
    • 流式细胞仪多色选择器
    • 试剂盒
    • 内参对照
    • 细胞裂解液
    • 多肽
    • 蛋白酶
    • 组织切片
    • 蛋白标签及细胞标记物
    • 相关试剂及工具
    帮助与支持
    • 技术支持
    • 技术FAQ
    • 实验步骤与建议
    • 购买FAQ
    • RabMAb 产品
    • 培训课程
    • 按靶标浏览
    企业
    • 企业资讯
    • 投资者关系
    • 公司新闻
    • 招贤纳士
    • 关于我们
    活动通知
    • 商品展览
    • 会议
    全球网站
    • abcam.com
    • abcam.co.jp
    QR code for Weechat

    微信扫码 关注我们

    ?ICP?14046373?-1

    ????? 31011502007413?

    • 销售条款
    • 网站使用条款
    • Cookie政策
    • 隐私权政策
    • 法律
    © 1998-2021 艾博抗(上海)贸易有限公司版权所有