Anti-HIF-2-alpha抗体[EPR19656] (ab207607)
概述
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产品名称Anti-HIF-2-alpha抗体[EPR19656]
参阅全部 HIF-2-alpha 一抗 -
描述兔单克隆抗体[EPR19656] to HIF-2-alpha
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宿主Rabbit
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经测试应用适用于: WB, ICC/IFmore details
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种属反应性与反应: Human
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免疫原
Recombinant fragment within Human HIF-2-alpha aa 600 to the C-terminus. The exact sequence is proprietary.
Database link: Q99814 -
阳性对照
- WB: Whole cell lysate of HeLa cells treated with 1mM deferoxamine (DFO) mesylate salt for 24h ; Whole cell lysate of HeLa cells treated with 100µM CoCl2 for 24h. ICC/IF: HeLa cells treated with DFO mesylate salt (1mM, 24h).
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常规说明
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents
This product is a recombinant rabbit monoclonal antibody.
性能
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形式Liquid
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存放说明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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存储溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
纯度Protein A purified
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克隆单克隆
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克隆编号EPR19656
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同种型IgG
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研究领域
相关产品
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Alternative Versions
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Compatible Secondaries
应用
Our Abpromise guarantee covers the use of ab207607 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| 应用 | Ab评论 | 说明 |
|---|---|---|
| WB | 1/1000. Detects a band of approximately 110 kDa (predicted molecular weight: 96 kDa). | |
| ICC/IF | 1/100. |
靶标
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功能Transcription factor involved in the induction of oxygen regulated genes. Binds to core DNA sequence 5'-[AG]CGTG-3' within the hypoxia response element (HRE) of target gene promoters. Regulates the vascular endothelial growth factor (VEGF) expression and seems to be implicated in the development of blood vessels and the tubular system of lung. May also play a role in the formation of the endothelium that gives rise to the blood brain barrier. Potent activator of the Tie-2 tyrosine kinase expression. Activation seems to require recruitment of transcriptional coactivators such as CREBPB and probably EP300. Interaction with redox regulatory protein APEX seems to activate CTAD.
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组织特异性Expressed in most tissues, with highest levels in placenta, lung and heart. Selectively expressed in endothelial cells.
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疾病相关Defects in EPAS1 are the cause of erythrocytosis familial type 4 (ECYT4) [MIM:611783]. ECYT4 is an autosomal dominant disorder characterized by increased serum red blood cell mass, elevated hemoglobin concentration and hematocrit, and normal platelet and leukocyte counts.
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序列相似性Contains 1 basic helix-loop-helix (bHLH) domain.
Contains 1 PAC (PAS-associated C-terminal) domain.
Contains 2 PAS (PER-ARNT-SIM) domains. -
翻译后修饰In normoxia, is probably hydroxylated on Pro-405 and Pro-531 by EGLN1/PHD1, EGLN2/PHD2 and/or EGLN3/PHD3. The hydroxylated prolines promote interaction with VHL, initiating rapid ubiquitination and subsequent proteasomal degradation. Under hypoxia, proline hydroxylation is impaired and ubiquitination is attenuated, resulting in stabilization.
In normoxia, is hydroxylated on Asn-847 by HIF1AN thus probably abrogating interaction with CREBBP and EP300 and preventing transcriptional activation.
Phosphorylated on multiple sites in the CTAD.
The iron and 2-oxoglutarate dependent 3-hydroxylation of asparagine is (S) stereospecific within HIF CTAD domains. -
细胞定位Nucleus.
- Information by UniProt
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数据库链接
- Entrez Gene: 2034 Human
- Omim: 603349 Human
- SwissProt: Q99814 Human
- Unigene: 468410 Human
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别名
- Basic helix loop helix PAS protein MOP2 antibody
- Basic-helix-loop-helix-PAS protein MOP2 antibody
- bHLHe73 antibody
see all
图片
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![Immunocytochemistry/ Immunofluorescence - Anti-HIF-2-alpha antibody [EPR19656] (ab207607)](https://www.abcam.cn/ps/products/207/ab207607/Images/ab207607-266329-207607IF.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-HIF-2-alpha antibody [EPR19656] (ab207607)Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized HeLa (Human epithelial cell line from cervix adenocarcinoma) cells, untreated or treated with deferoxamine mesylate salt (1mM, 24h), labeling HIF-2-alpha with ab207607 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green).
Confocal image showing nuclear staining on HeLa cells treated with deferoxamine mesylate salt (1mM, 24h).
The nuclear counterstain is DAPI (blue).
Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution (red).
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) at 1/1000 dilution.
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![Western blot - Anti-HIF-2-alpha antibody [EPR19656] (ab207607)](https://www.abcam.cn/ps/products/207/ab207607/Images/ab207607-266331-207607WBa.jpg)
Western blot - Anti-HIF-2-alpha antibody [EPR19656] (ab207607)All lanes : Anti-HIF-2-alpha antibody [EPR19656] (ab207607) at 1/1000 dilution
Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Whole cell lysate of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with 1mM deferoxamine (DFO) mesylate salt for 24h
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 96 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with the literature, and the expression level can be induced under hypoxia or treated with hypoxia mimetic chemicals such as DFO (PMID: 25587023, 10934146, 23958427 and 21571835).
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![Western blot - Anti-HIF-2-alpha antibody [EPR19656] (ab207607)](https://www.abcam.cn/ps/products/207/ab207607/Images/ab207607-266330-207607WBb.jpg)
Western blot - Anti-HIF-2-alpha antibody [EPR19656] (ab207607)All lanes : Anti-HIF-2-alpha antibody [EPR19656] (ab207607) at 1/1000 dilution
Lane 1 : Untreated HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : Whole cell lysate of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells treated with 100µM CoCl2 for 24h
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size: 96 kDa
Observed band size: 110 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with the literature, and the expression level can be induced under hypoxia or treated with hypoxia mimetic chemicals such as CoCl2 (PMID: 21571835, 10934146 and 23958427).
数据表及文件
文献
ab207607 has not yet been referenced specifically in any publications.
