Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (4-12% acrylamide)
Sample
Human Cell lysate - whole cell (Renal cancer cell line)
Specification
Renal cancer cell line
Blocking step
Li-cor Blocking Buffer PBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Dilution
1/500
Secondary antibody
Name
Abcam antibody:Donkey Anti-Mouse IgG H&L (Alexa Fluor® 790) preadsorbed
Dilution
1/5000
Detection
Detection method
LICOR
Bands
Specific: 100 kDa Non-specific: 35 kDa
Exposure
5 minute(s) and 0 second(s)
Negative control
Line. WT-Cells
Positive control
Line 1. VHL-KO cells
Additional data
Additional Notes
The antibody has a strong non-specific band at 35KDa.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Jul 28 2014