Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Infarct core striate)
Specification
Infarct core striate
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 10mM Citrate pH6
Permeabilization
No
Other product details
Dilution
1/1000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: Antibody diluent (non-Abcam)
Secondary antibody
Name
Non-Abcam antibody was used: Rabbit anti-mouse IgG whole molecule
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/200
Additional data
Additional Notes
It works great with immunofluorescence at same dilution and with secondary goat anti-mouse alexa 488 or 546.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 May 02 2008