Anti-HHV8抗体[LN53] (ab4103)

Submit a review Q&A (5)References (6)

Key features and details

  • Rat monoclonal [LN53] to HHV8
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Species independent
  • Isotype: IgG2c

概述

  • 产品名称

    Anti-HHV8抗体[LN53]
    参阅全部 HHV8 一抗

  • 描述

    大鼠单克隆抗体[LN53] to HHV8

  • 宿主

    Rat

  • 特异性

    HHV 8 is the likely etiological agent of Kaposi’s sarcoma (KS). HHV 8 encodes a latent nuclear antigen (LNA), which is the product of the viral gene orf 73. LNA is capable of forming a complex with retinoblastoma susceptibility gene product, which may be related to its oncogenic activity.

  • 经测试应用

    适用于: IHC-P, IHC-Frmore details

  • 种属反应性

    与反应: Species independent

  • 免疫原

    Recombinant protein corresponding to the latent nuclear antigen 1 molecule of HHV 8.

  • 表位

    Located within the ORF-73 region.

  • 阳性对照

    • Kaposi’s sarcoma.

  • 常规说明

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

性能

  • 形式

    Liquid

  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.

  • 存储溶液

    Preservative: 0.05% Sodium azide
    Constituents: Tissue culture supernatant, 1% BSA
  • Concentration information loading...

  • 纯度

    IgG fraction

  • 克隆

    单克隆

  • 克隆编号

    LN53

  • 同种型

    IgG2c

  • 研究领域

应用

Our Abpromise guarantee covers the use of ab4103 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P 1/25 - 1/50. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. ABC method.
IHC-Fr 1/25 - 1/50. ABC method.

靶标

  • 相关性

    HHV8 has been found to be associated with three different diseases observed in AIDS patients; kaposi's sarcoma, primary effusion lymphoma (which is a rare type of non-Hodgkin lymphoma affecting the body cavities) and multicentric Castleman's disease. To date there is much evidence to support a direct role for HHV8 in kaposi's sarcoma.

  • 别名

    • HHV 8 antibody
    • Human Herpes Virus 8 antibody
    • Human herpesvirus 8 antibody
    • Kaposi's sarcoma associated herpes virus antibody
    • KSHV antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHV8 antibody [LN53] (ab4103)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HHV8 antibody [LN53] (ab4103)
    Cultured cells infected with HHV8, stained with HHV8 using ABC and DAB chromogen.

文献 (6)

发表研究结果有使用 ab4103？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab4103 被引用在 6 文献中.

  • Naipauer J  et al. PDGFRA defines the mesenchymal stem cell Kaposi's sarcoma progenitors by enabling KSHV oncogenesis in an angiogenic environment. PLoS Pathog 15:e1008221 (2019). PubMed: 31881074
  • Tan B  et al. Viral and cellular N6-methyladenosine and N6,2'-O-dimethyladenosine epitranscriptomes in the KSHV life cycle. Nat Microbiol 3:108-120 (2018). PubMed: 29109479
  • Yang J  et al. Case report of ocular Kaposi's sarcoma. BMC Ophthalmol 17:143 (2017). PubMed: 28806949
  • Zhu Y  et al. An Oncogenic Virus Promotes Cell Survival and Cellular Transformation by Suppressing Glycolysis. PLoS Pathog 12:e1005648 (2016). IHC-P . PubMed: 27187079
  • El Hajj H  et al. Combination of arsenic and interferon-a inhibits expression of KSHV latent transcripts and synergistically improves survival of mice with primary effusion lymphomas. PLoS One 8:e79474 (2013). ICC/IF . PubMed: 24250827
  • Loddenkemper C  et al. [The morphologic variants of KSHV/HHV 8-associated lymphoproliferations.] Pathologe : (2006). PubMed: 17160657

客户评价及客户问答

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Question


I would like to know which region of LANA this antibody recognizes, N-terminal, C-terminal or central repeat region?

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Answer


The specific epitope of ab4103 is near the C terminus of the ORF-73 region within HHV8. Below is more information about specific recombinant proteins that this antibody recognizes.

GST-C14, GST-C17, GST-C7, and TH-C14;

GST-C14 contains amino acids 803 to 1113 of orf73 encompassing 126 amino acids of the central repeat domain as well as 184 amino acids of the C-terminal basic domain.

GST-C17 contains amino acids 803 to 942 comprised almost entirely of repetitive coding sequence.

GST-C14 served as the backbone for making further defined deletions of the C terminus of orf73 by using three unique restriction enzyme sites.

GST-C7 was constructed by digesting GST-C14 with NruI (in orf73) and NotI (in pGEX-4T3 [Pharmacia]), followed by end repair and ligation.

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Question

A happy new year to you and all at Abcam.

We have an inquiry for the HHV8 - LANA1 antibody. The customer has used Leica before. Kindly advise, from the many different HHV8 antibodies that Abcam have, which one matches the Leica HHV8-LNA, Clone 13B10, so we can quote.

Best regards

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Answer

Thank you for contacting us.

We do not have clone 13B10 in catalogue like the Leica microsystem. We however have antibody that is specific to HHV8- latent nuclear antigen that can quoted to customer;

https://www.abcam.com/hhv8-antibody-ln35-ab4103.html

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Customer contacted us regarding location of epitope on HHV8 sequence.

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Answer



The laboratory responded that the epitope for ab4103 is near the C terminus of ORF 73 within HHV8.

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Question

Please provide list of antibodies to HHV8 virus

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Answer

Thank you for your telephone enquiry yesterday afternoon. As requested, here is a list of all our antibodies for detection of HHV8 virus. I can recommend to review the online datasheets for further information. ab4103 Rat monoclonal Anti-HHV8 antibody [LN35] Applications: IHC-Fr, IHC-P Click here (or use the following: https://www.abcam.com/index.html?datasheet=4103). ab12208 Rat monoclonal Anti-HHV8 Cyclin antibody [94B] Applications: IF, IP, WB Click here (or use the following: https://www.abcam.com/index.html?datasheet=12208). ab25122 Rabbit polyclonal Anti-HHV8 GPCR antibody Applications: Flow Cyt Click here (or use the following: https://www.abcam.com/index.html?datasheet=25122). ab16159 Sheep polyclonal Anti-HHV8 ORF62 antibody Applications: WB Click here (or use the following: https://www.abcam.com/index.html?datasheet=16159). I hope this will be helpful to you. If you have any further questions, please do not hesitate to contact us.

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Question

has this antibody cross-reactivity with mouse antibody in a double staining immunofluorescence?

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Answer

Thank you for your enquiry and your patience in awaiting a response. HHV8 is human herpes virus and was tested only on human infected tissue. It was not tested for any other virus infected animal tissues. If you have any further questions then please do not hesitate to get back in touch with us.

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