Anti-hHR23A抗体(ab3836)

Submit a review Q&A (1)
Western blot - Anti-hHR23A antibody (ab3836)

    Key features and details

    • Goat polyclonal to hHR23A
    • Suitable for: WB
    • Reacts with: Human
    • Isotype: IgG

    概述

    • 产品名称

      Anti-hHR23A抗体
      参阅全部 hHR23A 一抗

    • 描述

      山羊多克隆抗体to hHR23A

    • 宿主

      Goat

    • 特异性

      This antibody has been shown to detect an approxiametely 60kd band in HeLa and Jurkat lysates. This is comparable in size to the band detected in HeLa in van der Spek et al. 1996. We believe this is good evidence that this antibody is detecting hHR23A.

    • 经测试应用

      适用于: WBmore details

    • 种属反应性

      与反应: Human
      预测可用于: Mouse

    • 免疫原

      Synthetic peptide:

      KSPSEESAPTTSPEC

      , corresponding to amino acids 121-133 of Human hHR23A.
      Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

    • 常规说明

      The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

      If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

    性能

    • 形式

      Liquid

    • 存放说明

      Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.

    • 存储溶液

      Preservative: 0.01% Sodium azide
      Constituents: 0.42% Potassium phosphate, 0.87% Sodium chloride
    • Concentration information loading...

    • 纯度

      Immunogen affinity purified

    • 克隆

      多克隆

    • 同种型

      IgG

    • 研究领域

    应用

    The Abpromise guarantee

    Abpromise™承诺保证使用ab3836于以下的经测试应用

    “应用说明”部分 下显示的仅为推荐的起始稀释度；实际最佳的稀释度/浓度应由使用者检定。

    应用 Ab评论 说明
    WB
    1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 40 kDa).
    说明
    WB
    1/500. Detects a band of approximately 60 kDa (predicted molecular weight: 40 kDa).

    靶标

    • 功能

      Multiubiquitin chain receptor involved in modulation of proteasomal degradation. Binds to 'Lys-48'-linked polyubiquitin chains in a length-dependent manner and with a lower affinity to 'Lys-63'-linked polyubiquitin chains. Proposed to be capable to bind simultaneously to the 26S proteasome and to polyubiquitinated substrates and to deliver ubiquitinated proteins to the proteasome.
      Involved in nucleotide excision repair and is thought to be functional equivalent for RAD23B in global genome nucleotide excision repair (GG-NER) by association with XPC. In vitro, the XPC:RAD23A dimer has NER activity. Can stabilize XPC.
      Involved in vpr-dependent replication of HIV-1 in non-proliferating cells and primary macrophages. Required for the association of HIV-1 vpr with the host proteasome.

    • 序列相似性

      Belongs to the RAD23 family.
      Contains 2 UBA domains.
      Contains 1 ubiquitin-like domain.

    • 结构域

      The ubiquitin-like domain mediates interaction with ATXN3.
      The ubiquitin-like (UBL) and the UBA (ubiquitin-associated) domains interact intramolecularly in a highly dynamic manner, as each UBA domain competes for an overlapping UBL domain surface. Binding of ubiquitin or proteasome subunit PSMD4 disrupt the UBL-UBA domain interactions and drive RAD23A in to an open conformation.

    • 翻译后修饰

      Phosphorylated upon DNA damage, probably by ATM or ATR.

    • 细胞定位

      Nucleus.
    • Information by UniProt

    • 数据库链接

      • 别名

        • hHR 23A antibody
        • hHR23A antibody
        • HR23A antibody
        • MGC111083 antibody
        • RAD 23a antibody
        • RAD23 homolog A (S. cerevisiae) antibody
        • RAD23 homolog A antibody
        • RAD23 yeast homolog A antibody
        • RAD23A antibody
        • RD23A_HUMAN antibody
        • UV excision repair protein RAD23 antibody
        • UV excision repair protein RAD23 homolog A antibody
        see all

      图片

      • Western blot - Anti-hHR23A antibody (ab3836)
        Western blot - Anti-hHR23A antibody (ab3836)

        Western blot using ab3836 at 1/500.
        Secondary ab: Rabbit anti Goat ab6741 1/5000
        Exposure time:30 seconds

        Lane 1: Hela nuclear
        Lane 2: Hela
        Lane 3: A431
        Lane 4: Jurkat
        Lane 5: 293

      文献 (0)

      发表研究结果有使用 ab3836？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab3836 尚未被引用在任何文献中。

      客户评价及客户问答

      Show All 评价 Q&A
      提交评价 提交问题

      Question

      I was wondering how much total protein was used each lane to make the figure in the datasheet of the product ab3836 to detect the endogenous hHR23a?

      Read More
      Answer

      Thank you for your enquiry. Our source for this antibody does not have the data for the amount of lysate used for this particular blot but reports that they typically load 30ug of cell lysate per lane for testing. I hope this helps. Please do not hesitate to contact us if you have further questions.

      Read More

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com