Anti-Hexokinase 1抗体(ab150013)
Key features and details
- Rabbit polyclonal to Hexokinase 1
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Hexokinase 1抗体
参阅全部 Hexokinase 1 一抗 -
描述
兔多克隆抗体to Hexokinase 1 -
宿主
Rabbit -
经测试应用
适用于: WBmore details -
种属反应性
与反应: Human
预测可用于: Mouse, Rat, Rabbit, Horse, Cow, Dog, Chimpanzee, Macaque monkey, Gorilla, Orangutan -
免疫原
Synthetic peptide corresponding to Human Hexokinase 1 aa 50-150 conjugated to keyhole limpet haemocyanin.
(Peptide available asab134654) -
阳性对照
- This antibody gave a positive signal in Hexokinase 1 Recombinant Protein and Human Testis tissue lysate as well as the following whole cell lysates: HeLa; MCF7; K562.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab150013于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa).
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 102 kDa (predicted molecular weight: 102 kDa). |
靶标
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组织特异性
Isoform 2 is erythrocyte specific. Isoform 3 and isoform 4 are testis-specific. -
通路
Carbohydrate metabolism; hexose metabolism. -
疾病相关
Hexokinase deficiency
Neuropathy, hereditary motor and sensory, Russe type -
序列相似性
Belongs to the hexokinase family.
Contains 2 hexokinase domains. -
结构域
The N- and C-terminal halves of this hexokinase show extensive sequence similarity to each other. The catalytic activity is associated with the C-terminus while regulatory function is associated with the N-terminus. Each domain can bind a single glucose and Gluc-6-P molecule. -
细胞定位
Mitochondrion outer membrane. Its hydrophobic N-terminal sequence may be involved in membrane binding. - Information by UniProt
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数据库链接
- Entrez Gene: 3098 Human
- Entrez Gene: 15275 Mouse
- Entrez Gene: 25058 Rat
- Omim: 142600 Human
- SwissProt: P19367 Human
- SwissProt: P17710 Mouse
- SwissProt: P05708 Rat
- Unigene: 370365 Human
see all -
别名
- BB404130 antibody
- Brain form hexokinase antibody
- dea antibody
see all
图片
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All lanes : Anti-Hexokinase 1 antibody (ab150013) at 1 µg/ml
Lane 1 : Human testis tissue lysate - total protein (ab30257)
Lane 2 : HeLa (Human epithelial carcinoma cell line) Whole Cell Lysate
Lane 3 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
Lane 4 : K562 (Human erythromyeloblastoid leukemia cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 102 kDa
Additional bands at: 46 kDa, 60 kDa, 75 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 16 minutes -
Anti-Hexokinase 1 antibody (ab150013) at 1/1 dilution +
Recombinant human Hexokinase 1 protein (ab85918) at 0.1 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 102 kDa
Observed band size: 102 kDa
Exposure time: 1 minute
数据表及文件
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SDS download
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Datasheet download
文献 (0)
ab150013 尚未被引用在任何文献中。