抗丙型肝炎病毒Core 1b抗体[C7-50] (ab2740)
Key features and details
- Mouse monoclonal [C7-50] to Hepatitis C Virus Core 1b
- Suitable for: IP, Flow Cyt, ELISA, WB
- Reacts with: Hepatitis C virus
- Isotype: IgG1
概述
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产品名称
抗丙型肝炎病毒Core 1b抗体[C7-50] -
描述
小鼠单克隆抗体[C7-50] to丙型肝炎病毒Core 1b -
宿主
Mouse -
特异性
Detects hepatitis C virus (HCV) core protein from transfected human and primate cell lines. -
经测试应用
适用于: IP, Flow Cyt, ELISA, WBmore details -
种属反应性
与反应: Hepatitis C virus -
免疫原
Recombinant full length protein (GST-tag) corresponding to Hepatitis C Virus Core 1b.
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表位
This antibody recognizes an epitope between amino acid residues 21-40 of HCV core protein. This sequence is conserved among different HCV strains. -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.
One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.
Learn more here.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
C7-50 -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab2740 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
应用 | Ab评论 | 说明 |
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IP |
Use at an assay dependent concentration.
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Flow Cyt |
1/100.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
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ELISA |
Use at an assay dependent concentration.
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WB | (1) |
Use a concentration of 1 µg/ml. This antibody detects a single ~21 kDa protein representing HCV core protein in various transfected cell lines.
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说明 |
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IP
Use at an assay dependent concentration. |
Flow Cyt
1/100. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ELISA
Use at an assay dependent concentration. |
WB
Use a concentration of 1 µg/ml. This antibody detects a single ~21 kDa protein representing HCV core protein in various transfected cell lines. |
靶标
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相关性
HCV (Hepatitis C Virus) viral core protein forms the internal viral coat that encapsidates the genomic RNA and is enveloped in a host cell-derived lipid membrane. The hepatitis C virus (HCV) core protein represents the first 177 amino acids of the viral precursor polyprotein and is cotranslationally inserted into the membrane of the endoplasmic reticulum. The N terminus of the core protein is involved in transcriptional repression. There are over 20 different subtypes of Hepatitis C Virus; HCV type 1b is mostly found in Europe and Asia. The prevalence of HCV type 1b infection has recently decreased, although it still accounts for most HCV-related cirrhosis and hepatocellular carcinoma. High HCV viremia levels and HCV genotype type 1b are independent predictors for poor response to interferon-alpha therapy. HCV core protein is among the most conserved proteins in HCV and is known to induce sensitization of cytotoxic T lymphocytes (CTL). Therefore, it is a prime candidate for a component of a potential HCV vaccine. -
细胞定位
Secreted; Host endoplasmic reticulum membrane; Single-pass membrane protein; Host nucleus; Host cytoplasm. -
数据库链接
- Entrez Gene: 951475 Hepatitis C virus
- SwissProt: P26662 Hepatitis C virus
- SwissProt: P26663 Hepatitis C virus
- SwissProt: Q9WMX2 Hepatitis C virus
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别名
- Capsid protein C antibody
- Core protein p19 antibody
- Core protein p21 antibody
- Hepatitis C virus core protein antibody
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (76)
ab2740 被引用在 76 文献中.
- Liu D et al. Effect of P-body component Mov10 on HCV virus production and infectivity. FASEB J N/A:N/A (2020). PubMed: 32496609
- Ma YY et al. IL28A protein homotetramer structure is required for autolysosomal degradation of HCV-NS5A in vitro. Cell Death Dis 11:200 (2020). PubMed: 32205851
- Li H et al. A proof-of-concept study in HCV-infected Huh7.5 cells for shortening the duration of DAA-based triple treatment regimens. Biomed Pharmacother 116:108976 (2019). PubMed: 31103827
- Mata M et al. Impact of a patient-derived hepatitis C viral RNA genome with a mutated microRNA binding site. PLoS Pathog 15:e1007467 (2019). PubMed: 31075158
- Bian WX et al. Binding of cellular nucleolin with the viral core RNA G-quadruplex structure suppresses HCV replication. Nucleic Acids Res 47:56-68 (2019). PubMed: 30462330