抗乙型肝炎病毒X antigen抗体[X36C] (ab2741)
Key features and details
- Mouse monoclonal [X36C] to Hepatitis B Virus X antigen
- Suitable for: IHC-P, ELISA, IP, WB
- Reacts with: Hepatitis B virus
- Isotype: IgG1
概述
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产品名称
抗乙型肝炎病毒X antigen抗体[X36C]
参阅全部 Hepatitis B Virus X antigen 一抗 -
描述
小鼠单克隆抗体[X36C] to乙型肝炎病毒X antigen -
宿主
Mouse -
经测试应用
适用于: IHC-P, ELISA, IP, WBmore details -
种属反应性
与反应: Hepatitis B virus -
免疫原
Recombinant full length protein corresponding to Hepatitis B Virus X antigen.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
Preservative: 0.05% Sodium azide
Constituent: PBS -
Concentration information loading...
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纯度
HPTLC -
Primary antibody说明
Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein-protein interaction. -
克隆
单克隆 -
克隆编号
X36C -
同种型
IgG1 -
研究领域
相关产品
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Compatible Secondaries
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Conjugation kits
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Isotype control
应用
应用 | Ab评论 | 说明 |
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IHC-P |
Use at an assay dependent concentration.
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ELISA |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
Use a concentration of 1 µg/ml.
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说明 |
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IHC-P
Use at an assay dependent concentration. |
ELISA
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use a concentration of 1 µg/ml. |
靶标
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相关性
Hepatitis B virus X protein (HBx) is a 17 kD transcriptional coactivator that plays a significant role in the regulation of genes involved in inflammation and cell survival. It regulates many transcription factors including nuclear factor kappa B (NF-kappaB) and plays a key role in hepatocarcinogenesis. HBx facilitates the binding of cAMP response element binding protein (CREB) to its responsive element. HBx stabilizes the cellular coactivator ASC-2 through direct protein-protein interaction, affecting the regulation of genes actively transcribed in liver cancer cells. HBx transactivates both JNK and MAPK signal transduction pathways in association with the mobilization of cytosolic Ca2+. The communication between HBx and general transcription factor TFIIB is also one of the mechanisms which account for its transcriptional transactivation. HBx decreased the expression of PTEN a known tumor suppressor and a negative regulator of phosphatidylinositol 3'-kinase/AKT and HBx decreased the expression of PTEN in HBx-transfected cells. The etiology of hepatocellular carcinoma (HCC) is involved with hepatitis B virus (HBV) infection and HBx in particular plays a role in the development of HBV-related HCC. The persistence of HBx is important to the pathogenesis of early HCC and HBx expression in the liver during chronic HBV infection may be an important prognostic marker for the development of HCC. -
数据库链接
- SwissProt: O91531 Hepatitis B virus
- SwissProt: Q4R1S1 Hepatitis B virus
- SwissProt: Q91C38 Hepatitis B virus
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别名
- HBV X antigen antibody
- HBx antibody
- HBxAg antibody
实验方案
数据表及文件
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SDS download
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Datasheet download
文献 (7)
ab2741 被引用在 7 文献中.
- You DG et al. Hepatitis B virus X protein induces size-selective membrane permeabilization through interaction with cardiolipin. Biochim Biophys Acta Biomembr 1861:729-737 (2019). PubMed: 30658058
- Zha Y et al. Hepatitis B virus X protein promotes epithelial-mesenchymal transition and metastasis in hepatocellular carcinoma cell line HCCLM3 by targeting HMGA2. Oncol Lett 16:5709-5714 (2018). PubMed: 30356986
- Lee HR et al. A direct role for hepatitis B virus X protein in inducing mitochondrial membrane permeabilization. J Viral Hepat 25:412-420 (2018). PubMed: 29193612
- Wang B et al. Hepatitis B virus infection is not associated with fatty liver disease: Evidence from a cohort study and functional analysis. Mol Med Rep N/A:N/A (2018). PubMed: 30387826
- Zhong L et al. Reactive Oxygen Species-Mediated c-Jun NH2-Terminal Kinase Activation Contributes to Hepatitis B Virus X Protein-Induced Autophagy via Regulation of the Beclin-1/Bcl-2 Interaction. J Virol 91:N/A (2017). PubMed: 28515304
- Huang P et al. Hepatitis B Virus X Protein (HBx) Is Responsible for Resistance to Targeted Therapies in Hepatocellular Carcinoma: Ex Vivo Culture Evidence. Clin Cancer Res 21:4420-30 (2015). PubMed: 26059188
- Yip WK et al. Carboxyl-terminal truncated HBx regulates a distinct microRNA transcription program in hepatocellular carcinoma development. PLoS One 6:e22888 (2011). WB . PubMed: 21829663