抗乙型肝炎病毒Surface Antigen抗体[3E7]，prediluted (ab859)

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Key features and details

  • Mouse monoclonal [3E7] to Hepatitis B Virus Surface Antigen, prediluted
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Hepatitis B virus
  • Isotype: IgG1

概述

  • 产品名称

    抗乙型肝炎病毒Surface Antigen抗体[3E7]，prediluted
    参阅全部 Hepatitis B Virus Surface Antigen 一抗

  • 描述

    小鼠单克隆抗体[3E7] to乙型肝炎病毒Surface Antigen，prediluted

  • 宿主

    Mouse

  • 特异性

    This antibody stains the cytoplasm of antigen-positive liver cells in patients with Type B viral hepatitis. This antibody reacts with the ‘a’ determinant present on the HBsAg subtypes ayw1, ayw2, ayw3, ayw4, ayr, adw2, adw4 and adr. It does not react with normal tissues.

  • 经测试应用

    适用于: IHC-P, IHC-Frmore details

  • 种属反应性

    与反应: Hepatitis B virus

  • 免疫原

    BALB/c mice were immunized with purified HBsAG subtype AYW3.

  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing the problem with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation.

    One factor contributing to the crisis is the use of antibodies that are not suitable. This can lead to misleading results and the use of incorrect data informing project assumptions and direction. To help address this challenge, we have introduced an application and species grid on our primary antibody datasheets to make it easy to simplify identification of the right antibody for your needs.

    Learn more here.

性能

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab859 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
IHC-P
IHC-Fr
说明
  • 应用说明
    This antibody has been pretitered and quality controlled to work on formalin-fixed paraffin-embedded as well as acetone fixed cryostat tissue sections.
    No further titration is required.
    We suggest an incubation period of 60 minutes at room temperature.
    However, depending upon the fixation conditions and the staining system employed, optimal incubation should be determined by the user.

    • 靶标

    • 相关性

      Hepatitis B Virus (HBV) infection induces a disease state which manifests itself in a variety of ways, characterized by the extent of liver damage, inflammation and viral persistence. HBV infection is also associated with a 100 fold increased risk of hepatocellular carcinoma and currently infects over 250 million people worldwide. HBV has a partially double stranded 3.2 kilobase DNA genome which contains four open reading frames. One of these encodes a 154 amino acid protein called the HBx protein. HBx has been shown to be a transcriptional transactivator of both viral and cellular promoters. Lacking a DNA binding domain and nuclear localization signal, HBx is believed to exert transcriptional activity through protein protein interaction.

    • 细胞定位

      Virion membrane

    • 数据库链接

      • 别名

        • Large envelope protein antibody
        • Large surface protein antibody
        • LHB antibody
        • Major surface antigen antibody

      文献 (0)

      发表研究结果有使用 ab859？请让我们知道，以便我们可以引用本数据表中的参考文章。

      ab859 尚未被引用在任何文献中。

      客户评价及客户问答

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      Question

      Hi Abcam Tech Support Team,

      May I know which Human HBsAg Monoclonal antibody (i.e. ab110600, ab54247 and ab20724) binds to all four serotypes of Hepatitis B virus?

      I look forward to receiving your reply soon. Many thanks in advance!

      Best regards,

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      Answer

      Thank you for your email.
      We do offer a variety of antibodies that will recognize the four major serotypes: adr, adw, ayr, ayw.
      If it has been tested which serotype is recognizwed by an antibody this information can be found on the datasheet in the "specificity" section.
      Here is a non- exhaustive list of antibodies that bind all four major serotypes. They have even been characterized further and the datasheet will tell you the serotype subtypes (e.g. ayw2, ayw3, ayw4, ayr, adw2, adw4, adrq+, adrq-)also.
      For example:
      ab2039
      https://www.abcam.com/Hepatitis-B-Virus-Surface-Antigen-antibody-HB12-ab2039.html (or use the following: https://www.abcam.com/Hepatitis-B-Virus-Surface-Antigen-antibody-HB12-ab2039.html).
      ab8333
      https://www.abcam.com/Hepatitis-B-Virus-Surface-Antigen-antibody-HB5-ab8333.html (or use the following: https://www.abcam.com/Hepatitis-B-Virus-Surface-Antigen-antibody-HB5-ab8333.html).
      ab859
      https://www.abcam.com/Hepatitis-B-Virus-Surface-Antigen-antibody-3E7-prediluted-ab859.html (or use the following: https://www.abcam.com/Hepatitis-B-Virus-Surface-Antigen-antibody-3E7-prediluted-ab859.html).
      ab20724
      https://www.abcam.com/Hepatitis-B-Virus-Surface-Antigen-Ayw-antibody-HB5-ab20724.html (or use the following: https://www.abcam.com/Hepatitis-B-Virus-Surface-Antigen-Ayw-antibody-HB5-ab20724.html).
      Which antibody is the most suitable for experiments depends on your samples and the planned application.
      We encourage customers to consult the latest literature available through PubMed and other resources in order to find the most up-to-date information about this specific research topic.
      I hope this information is helpful. Please do not hesiate to contact me again with anyfurther questions.

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      Question

      ab859 Hepatitis B Virus Surface Antigen antibody [3E7], prediluted Can you provide the concentration information?

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      Answer

      Regarding ab859 (Hepatitis B Virus Surface Antigen antibody [3E7], prediluted), the concentration of the present lot is 2 ug/ml. Please don't hesitate to contact us again if you have further questions.

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      Question

      Hello, Just had a quick question about product ab859. This is a pre-diluted antibody - What is the final concentration? Thanks, Mike

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      Answer

      The final concentration of this prediluted antibody is 20ug/ml.

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