Anti-Heparanase 1抗体(ab85543)
Key features and details
- Rabbit polyclonal to Heparanase 1
- Suitable for: WB, IHC-P
- Reacts with: Human, Recombinant fragment
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Heparanase 1抗体
参阅全部 Heparanase 1 一抗 -
描述
兔多克隆抗体to Heparanase 1 -
宿主
Rabbit -
特异性
ab85543 recognizes the 65kD precursor as well as the 50kD and 8kD subunits of Heparanase 1. -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Human, Recombinant fragment -
免疫原
Recombinant full length protein corresponding to Human Heparanase 1.
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阳性对照
- Human spleen tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
存储溶液
pH: 7.20
Preservative: 0.01% Thimerosal (merthiolate)
Constituent: PBS -
Concentration information loading...
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纯度
Protein G purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85543于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500. Predicted molecular weight: 62 kDa.
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IHC-P | (1) |
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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说明 |
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WB
1/500. Predicted molecular weight: 62 kDa. |
IHC-P
Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
靶标
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功能
Endoglycosidase which is a cell surface and extracellular matrix-degrading enzyme. Cleaves heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. Also implicated in the extravasation of leukocytes and tumor cell lines. Due to its contribution to metastasis and angiogenesis, it is considered to be a potential target for anti-cancer therapies. -
组织特异性
Highly expressed in placenta and spleen and weakly expressed in lymph node, thymus, peripheral blood leukocytes, bone marrow, endothelial cells, fetal liver and tumor tissues. -
序列相似性
Belongs to the glycosyl hydrolase 79 family. -
翻译后修饰
Proteolytically processed. The cleavage of the 65 kDa form leads to the generation of a linker peptide, 8 kDa and 50 kDa product. The active form, the 8/50 kDa heterodimer, is resistant to degradation. Complete removal of the linker peptide appears to be a prerequisite to the complete activation of the enzyme.
N-glycosylated. Glycosylation of the 50 kDa subunit appears to be essential for its solubility. -
细胞定位
Lysosome membrane. Secreted. Secreted, internalised and transferred to late endosomes/lysosomes as a proheparanase. In lysosomes, it is processed into the active form, the heparanase. The uptake or internalisation of proheparanase is mediated by HSPGs. Heparin appears to be a competitor and retain proheparanase in the extracellular medium. - Information by UniProt
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数据库链接
- Entrez Gene: 10855 Human
- Omim: 604724 Human
- SwissProt: Q9Y251 Human
- Unigene: 44227 Human
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别名
- Endo glucoronidase antibody
- Endo-glucoronidase antibody
- HEP antibody
see all
图片
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ab85543 staining Heparanase 1 in Human lung tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% NHS for 30 minutes at room temperature; antigen retrieval was by heat mediation with an EDTA buffer. Samples were incubated with primary antibody (1/4000 in NHS) for 8 hours. A Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/250) was used as the secondary antibody.
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Purified recombinant human heparanase served as a control (Lane1). Lane2: An extract from 6.5x10e3 CHO cells, transfected with human Heparanase; Lane3: extract from 8x10e4 CHO cells, transfected with mouse Heparanase, and Lane4: extract from 5x10e5 non-transfected CHO dhfr- cells, were loaded onto 4-12% NuPage gel. The proteins were transferred to a PVDF and subjected to Western Blot analysis using ab85543 at 1:1000 dilution. The 65kD precursor and the 50kD and 8kD subunits are clearly detected. Lane 1 : Purified recombinant human Heparanase served as a control
Lane 2 : Extract from 6.5x10e3 CHO cells, transfected with human Heparanase
Lane 3 : Extract from 8x10e4 CHO cells, transfected with mouse Heparanase
Lane 4 : Extract from 5x10e5 non-transfected CHO dhfr- cells
Cell were loaded onto 4-12% NuPage gel. The proteins were transferred to a PVDF and subjected to Western Blot analysis using ab85543 at 1:1000 dilution. The cleavage of the 65 kDa form leads -
ab85543, at 5µg/ml, staining Heparanase 1 in formalin-fixed, paraffin-embedded human spleen tissue by Immunohistochemistry.
数据表及文件
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SDS download
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Datasheet download
文献 (18)
ab85543 被引用在 18 文献中.
- Liu M et al. Heparanase modulates the prognosis and development of BRAF V600E-mutant colorectal cancer by regulating AKT/p27Kip1/Cyclin E2 pathway. Oncogenesis 11:58 (2022). PubMed: 36130926
- Cui Y et al. Upregulation of Fecal Epithelial Heparanase mRNA Is Associated with Increased Ulcerative Colitis Activity and Cancerization Risk. Dig Dis Sci 66:1488-1498 (2021). PubMed: 32445051
- Koujah L et al. Heparanase-Induced Activation of AKT Stabilizes β-Catenin and Modulates Wnt/β-Catenin Signaling during Herpes Simplex Virus 1 Infection. mBio 12:e0279221 (2021). PubMed: 34749529
- Wang C et al. Heparanase potentiates the invasion and migration of pancreatic cancer cells via epithelial-to-mesenchymal transition through the Wnt/ß-catenin pathway. Oncol Rep 44:711-721 (2020). PubMed: 32627022
- Zhang D et al. Crocin alleviates lipopolysaccharide-induced acute respiratory distress syndrome by protecting against glycocalyx damage and suppressing inflammatory signaling pathways. Inflamm Res 69:267-278 (2020). PubMed: 31925528