Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (kidney)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: 10µg/ml protease type XXIV
Permeabilization
No
Specification
kidney
Blocking step
Universal Blocking Reagent in aqua dest as blocking agent for 25 minute(s) · Concentration: 10% · Temperature: RT°C
Fixative
Formaldehyde
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 37°C · Diluent: universal blocking reagent 1/100 in PBS
Dilution
1/100
Secondary antibody
Dilution
1/100
Name
Non-Abcam antibody was used: rabbit anti rat biotin
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
- AB works also on rat tissues (tested on artery)
- Attention: negative control should be incubated extra because of condensation
- use DAB+ (Standard DAB is too weak)
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
FRAU. Barbara Rodenbeck
Verified customer
提交于 Oct 07 2020