概述

  • 产品名称

    Anti-Heme Oxygenase 1抗体[HO-1-1]
    参阅全部 Heme Oxygenase 1 一抗
  • 描述

    小鼠单克隆抗体[HO-1-1] to Heme Oxygenase 1
  • 宿主

    Mouse
  • 经测试应用

    适用于: WB, ICC, Flow Cyt, ELISA, IP, Sandwich ELISA, IHC-P, ICC/IF, IHC-Frmore details
  • 种属反应性

    与反应: Mouse, Rat, Cow, Dog, Human, Monkey
    预测可用于: Pig
  • 免疫原

    Synthetic peptide:

    MERPQPDSMPQDLSEALKEATKEVHTQAEN

    , corresponding to amino acids 1-30 of Human Heme Oxygenase 1.

  • 阳性对照

    • Recombinant Human or Rat HO-1 (Hsp32) Protein. HEK293 treated with 30 uM hemin for 18hrs (see Abreview). IHC-P: FFPE human spleen normal.
  • 常规说明

    This product was changed from ascites to tissue culture supernatant on 22nd May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

应用

Our Abpromise guarantee covers the use of ab13248 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

应用 Ab评论 说明
WB Use at an assay dependent concentration. Detects a band of approximately 32 kDa (predicted molecular weight: 34.6 kDa).
ICC Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

ELISA Use at an assay dependent concentration.
IP Use at an assay dependent concentration. PubMed: 24098580
Sandwich ELISA Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit polyclonal to Heme Oxygenase 1 (ab13243).

For sandwich ELISA, use this antibody as Capture at 5 µg/ml with Rabbit polyclonal to Heme Oxygenase 1 (ab13243) as Detection.

IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use at an assay dependent concentration.
IHC-Fr Use at an assay dependent concentration.

靶标

图片

  • All lanes : Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248) at 1 µg/ml

    Lane 1 : Hek293
    Lane 2 : HL60
    Lane 3 : HeLa
    Lane 4 : A549
    Lane 5 : Hu spleen
    Lane 6 : Ms spleen
    Lane 7 : Rt spleen

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : IRDye® 800CW Goat anti Mouse

    Predicted band size: 34.6 kDa
    Observed band size: 32 kDa
    why is the actual band size different from the predicted?



    Hek293 & HL60 presumed negative or very low expression.

    Loading control GAPDH at 38kDa

    This image was generated using the ascites version of the product.

  • IHC image of Hem Oxygensae 1 staining in a section of formalin fixed, paraffin embedded normal human spleen tissue section*, performed on a Leica Bond™ system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab13248, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    This image was generated using the ascites version of the product.

     

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

    *Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre

  • The following proteins and lysates were electrophoresed; Lane 1 - Heme-Oxygenase-1 (Hsp32) Protein (50ng), lane 2 - Heme-Oxygenase-2 protein NSP-550 (100ng), lane 3 - MDBK Cell Lysate (20ug), lane 4 - Mouse liver microsome (20ug) and lane 5 - Dog liver microsome (20ug). ab13248 was applied at a concentration of 4ugml-1.

    This image was generated using the ascites version of the product.

  • Standard Curve for Heme Oxygenase 1 (Analyte: Heme Oxygenase 1 protein (Tagged) (ab85243)); dilution range 1pg/ml to 1µg/ml using Capture Antibody Mouse monoclonal [HO-1-1] to Heme Oxygenase 1 (ab13248) at 5µg/ml and Detector Antibody Rabbit polyclonal to Heme Oxygenase 1 (ab13243) at 0.5µg/ml.

    This image was generated using the ascites version of the product.

  • Lane 1 : MW marker
    Lanes 2-7 : Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248)

    Lane 2 : Recombinant Rat Heme Oxygenase 1
    Lane 3 : Recombinant Human Heme Oxygenase 1
    Lane 4 : Recombinant Human Heme Oxygenase 2
    Lane 5 : MDBK cell lysate
    Lane 6 : Dog liver microsome
    Lane 7 : Mouse liver microsome

    Predicted band size: 34.6 kDa



    This image was generated using the ascites version of the product.

  • Anti-Heme Oxygenase 1 antibody [HO-1-1] (ab13248) at 1/250 dilution + Human microsome lysate

    Predicted band size: 34.6 kDa



    This image was generated using the ascites version of the product.

  • ab13248 staining Heme Oxygenase 1 in murine heart tissue by Immunohistochemistry (Frozen sections).Tissue was fixed in paraformaldehyde, permeabilized, blocked using 2% serum for 1 hour at 30°C and then incubated with ab13248 at a 1/300 dilution for 12 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat polyclonal used at a 1/500 dilution.The green colour indicate Heme Oxygenase 1.The blue colour indicate nucleus stain DAPI.

    This image was generated using the ascites version of the product.

    See Abreview

  • ab13248 at 10µg/ml staining Heme Oxygenase 1 in human lung cancer A2 cells by flow cytometery. The left image repersents staining with isotype control antibody and the right image show staining with ab13248.

    This image was generated using the ascites version of the product.

文献

This product has been referenced in:

  • Zhang B  et al. Notoginsenoside R1 Protects db/db Mice against Diabetic Nephropathy via Upregulation of Nrf2-Mediated HO-1 Expression. Molecules 24:N/A (2019). Read more (PubMed: 30634720) »
  • Wang X  et al. Effect of Gastrodin on Early Brain Injury and Neurological Outcome After Subarachnoid Hemorrhage in Rats. Neurosci Bull N/A:N/A (2019). Read more (PubMed: 30673960) »
See all 136 Publications for this product

客户评价及客户问答

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1-10 of 17 Abreviews

Application
Western blot
Sample
Rat Cell lysate - whole cell (PC12 cell)
Gel Running Conditions
Non-reduced Denaturing (12%)
Loading amount
20 µg
Specification
PC12 cell
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 27°C

Miss. Ji Yeon Lee

Verified customer

提交于 Jan 25 2019

Application
Western blot
Sample
Human Cell lysate - whole cell (DU145)
Gel Running Conditions
Reduced Denaturing (10)
Loading amount
25 µg
Treatment
20nM siNeg or siNFE2L2 for 24, 48 and 72h
Specification
DU145
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Jan 17 2019

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Spleen)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: pH 6.0 Citrate
Permeabilization
No
Specification
Spleen
Blocking step
PB ab64226 as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: RT°C
Fixative
Formaldehyde

Abcam user community

Verified customer

提交于 Jan 23 2018

Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (Skin)
Permeabilization
No
Specification
Skin
Blocking step
Serum as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Acetone

Abcam user community

Verified customer

提交于 Jul 14 2016

Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing (8%)
Sample
Rat Cell lysate - whole cell (RAT AORTIC SMOOTH MUSCLE CELLS)
Specification
RAT AORTIC SMOOTH MUSCLE CELLS
Blocking step
Milk as blocking agent for 3 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Mr. P Bingi

Verified customer

提交于 Oct 14 2013

Application
Western blot
Sample
Human Cell lysate - whole cell (Glioblastoma primary cells)
Loading amount
30 µg
Specification
Glioblastoma primary cells
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Apr 16 2013

Application
Western blot
Sample
Mouse Tissue lysate - other (skeletal muscle)
Loading amount
30 µg
Specification
skeletal muscle
Gel Running Conditions
Non-reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 30 minute(s) · Concentration: 5% · Temperature: 32°C

Mr. Mtuhtu S.Gounder

Verified customer

提交于 Jan 06 2012

Application
Western blot
Sample
Rat Cell lysate - whole cell (kidney)
Loading amount
20 µg
Specification
kidney
Treatment
Cadmium
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Dec 21 2011

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Heart)
Specification
Heart
Fixative
Paraformaldehyde
Permeabilization
Yes - To enchance Ab entry
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 30°C

Mr. Mtuhtu S.Gounder

Verified customer

提交于 Dec 19 2011

Application
Western blot
Sample
Human Cell lysate - whole cell (kidney)
Loading amount
20 µg
Specification
kidney
Treatment
Cadmium
Gel Running Conditions
Reduced Denaturing (4-12% gradient)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Dec 01 2011

1-10 of 17 Abreviews

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