Anti-Heme Oxygenase 1抗体(ab85309)
Key features and details
- Rabbit polyclonal to Heme Oxygenase 1
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Heme Oxygenase 1抗体
参阅全部 Heme Oxygenase 1 一抗 -
描述
兔多克隆抗体to Heme Oxygenase 1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-Pmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Rat Heme Oxygenase 1 aa 150-250 conjugated to keyhole limpet haemocyanin.
(Peptide available asab94374) -
常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab85309于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa).
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IHC-P | (3) |
Use at an assay dependent concentration.
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说明 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 33 kDa (predicted molecular weight: 33 kDa). |
IHC-P
Use at an assay dependent concentration. |
靶标
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功能
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. -
序列相似性
Belongs to the heme oxygenase family. -
细胞定位
Microsome. Endoplasmic reticulum. - Information by UniProt
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数据库链接
- Entrez Gene: 3162 Human
- Entrez Gene: 15368 Mouse
- Entrez Gene: 24451 Rat
- Omim: 141250 Human
- SwissProt: P09601 Human
- SwissProt: P14901 Mouse
- SwissProt: P06762 Rat
- Unigene: 517581 Human
see all -
别名
- 32 kD antibody
- bK286B10 antibody
- D8Wsu38e antibody
see all
图片
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All lanes : Anti-Heme Oxygenase 1 antibody (ab85309) at 1 µg/ml
Lane 1 : HEK-293
Lane 2 : HL60
Lane 3 : Rt spleen
Lane 4 : NR8383
Lane 5 : Ms spleen
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : IRDye® 800CW Goat anti Rabbit
Predicted band size: 33 kDa
Observed band size: 32 kDa why is the actual band size different from the predicted?Hek293 & HL60 presumed negative or very low expression.
Loading control GAPDH at 38kDa
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Immunohistochemistical detection of Anti-Heme Oxygenase 1 antibody (ab85309; 1/5000 for 2h @ 21°C) on human lung formalin/PFA-fixed paraffin-embedded sections. Antigen retrieval step:Heat mediated - Buffer/Enzyme Used: Citric acid.Blocking step: 1% BSA for 10 mins at 21°C. Secondary antibody biotin conjugated anti Rabbit IgG (1/200). NB: Positivity appears to be confined to macrophages- the primary antibody was omitted on the sequential section and was negative for macrophages ( to rule out lipofuscin).
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All lanes : Anti-Heme Oxygenase 1 antibody (ab85309) at 1 µg/ml
Lane 1 : Spleen (Rat) Tissue Lysate
Lane 2 : Heart (Rat) Tissue Lysate
Lane 3 : Lung (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 33 kDa
Additional bands at: 47 kDa, 55 kDa, 70 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
All lanes : Anti-Heme Oxygenase 1 antibody (ab85309) at 1 µg/ml
Lane 1 : Human spleen tissue lysate - total protein (ab29699)
Lane 2 : Human lung normal tissue lysate - total protein (40 - 65 years) (ab30281)
Lane 3 : Heart (Mouse) Tissue Lysate
Lane 4 : Spleen (Mouse) Tissue Lysate
Lane 5 :Mouse lung normal tissue lysate - total protein (ab29297)
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 33 kDa
Observed band size: 30 kDa why is the actual band size different from the predicted?
Additional bands at: 44 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 20 minutes -
Immunohistochemistical detection of Anti-Heme Oxygenase 1 antibody (ab85309; 1/5000 for 2h @ 21°C)on rat lung formalin/PFA-fixed paraffin-embedded sections. Antigen retrieval step:Heat mediated - Buffer/Enzyme Used: Citric acid.Blocking step: 1% BSA for 10 mins. Secondary antibody biotin conjugated anti Rabbit IgG (1/200). NB: As well as in macrophage cytoplasm, there is also clear positivity in the lining epitheium of the respiratory conducting tubes. I do not know if this is specific/non-specific.
数据表及文件
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SDS download
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Datasheet download
文献 (5)
ab85309 被引用在 5 文献中.
- Huang X et al. Transcriptome Analysis of Protection by Dendrobium Nobile Alkaloids (DNLA) against Chronic Alcoholic Liver Injury in Mice. Biomedicines 10:N/A (2022). PubMed: 36359319
- Sohn E et al. Ficus erecta Thunb Leaves Alleviate Memory Loss Induced by Scopolamine in Mice via Regulation of Oxidative Stress and Cholinergic System. Mol Neurobiol 58:3665-3676 (2021). PubMed: 33797061
- Li YY et al. Protective effects of HO-1 pathway on lung injury subsequent to limb ischemia reperfusion. Kaohsiung J Med Sci 35:417-424 (2019). PubMed: 30977589
- Chen X et al. Ethanol extract of Schisandrae chinensis fructus ameliorates the extent of experimentally induced atherosclerosis in rats by increasing antioxidant capacity and improving endothelial dysfunction. Pharm Biol 56:612-619 (2018). PubMed: 31070526
- Kataoka K et al. Visualization of Oxidative Stress Induced by Experimental Periodontitis in Keap1-Dependent Oxidative Stress Detector-Luciferase Mice. Int J Mol Sci 17:N/A (2016). IHC-P ; Mouse . PubMed: 27854327