Anti-Heme Oxygenase 1抗体(ab137749)
Key features and details
- Rabbit polyclonal to Heme Oxygenase 1
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
选择批间可重复性更高的重组抗体
- 研究可靠 —— 各批次间结果一致且可重复
- 长期批量供应 —— 采用重组技术,可实现快速生产
- 首次实验即可成功 —— 经过大量验证确认了特异性
- 符合伦理标准 —— 产品不含动物成分
概述
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产品名称
Anti-Heme Oxygenase 1抗体
参阅全部 Heme Oxygenase 1 一抗 -
描述
兔多克隆抗体to Heme Oxygenase 1 -
宿主
Rabbit -
经测试应用
适用于: WB, IHC-P, ICC/IFmore details -
种属反应性
与反应: Mouse, Rat, Human -
免疫原
Recombinant fragment, corresponding to a region within amino acids 1-278 of Human Heme Oxygenase 1.
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阳性对照
- H1299, HeLa, NIH-3T3, JC, BCL-1, C2C12 and Raw264.7 whole cell lysates, OV90 xenograft tissue.
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常规说明
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
存储溶液
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine) -
Concentration information loading...
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纯度
Immunogen affinity purified -
克隆
多克隆 -
同种型
IgG -
研究领域
相关产品
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab137749于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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WB |
1/500 - 1/3000. Predicted molecular weight: 33 kDa.
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IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used.
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ICC/IF |
1/100 - 1/1000.
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说明 |
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WB
1/500 - 1/3000. Predicted molecular weight: 33 kDa. |
IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Alternatively antigen retrieval using heat mediated Tris/EDTA buffer (pH 8.0) may be used. |
ICC/IF
1/100 - 1/1000. |
靶标
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功能
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. -
序列相似性
Belongs to the heme oxygenase family. -
细胞定位
Microsome. Endoplasmic reticulum. - Information by UniProt
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数据库链接
- Entrez Gene: 3162 Human
- Entrez Gene: 15368 Mouse
- Entrez Gene: 24451 Rat
- Omim: 141250 Human
- SwissProt: P09601 Human
- SwissProt: P14901 Mouse
- SwissProt: P06762 Rat
- Unigene: 517581 Human
see all -
别名
- 32 kD antibody
- bK286B10 antibody
- D8Wsu38e antibody
see all
图片
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All lanes : Anti-Heme Oxygenase 1 antibody (ab137749) at 1/500 dilution
Lane 1 : Wile-type HeLa cell lysate
Lane 2 : Knockout Hela cell lysates
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 33 kDa
Observed band size: 33 kDa -
Immunohistochemical analysis of human renal carcinaoma using ab137749 at 1/500.
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Immunofluorescent analysis of A375 cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ab137749 diluted at 1:500.
Blue: Hoechst 33342 staining. -
Immunohistochemical analysis of rat spleen using ab137749 at 1/500.
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All lanes : Anti-Heme Oxygenase 1 antibody (ab137749) at 1/5000 dilution
Lane 1 : Untreated HeLa whole cell extracts
Lane 2 : Treated HeLa whole cell extracts
Lysates/proteins at 30 µg per lane.
Secondary
All lanes : HRP-conjugated anti-rabbit IgG antibody
Predicted band size: 33 kDa -
All lanes : Anti-Heme Oxygenase 1 antibody (ab137749) at 1/1000 dilution
Lane 1 : H1299 cell lysate
Lane 2 : HeLa cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 33 kDa
12% SDS PAGE -
Immunohistochemical analysis of paraffin-embedded OV90 xenograft, labelling Heme Oxygenase 1 using ab137749 at 1/100 dilution.
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All lanes : Anti-Heme Oxygenase 1 antibody (ab137749) at 1/1000 dilution
Lane 1 : NIH-3T3 whole cell lysate
Lane 2 : JC whole cell lysate
Lane 3 : BCL1 whole cell lysate
Lane 4 : C2C12 whole cell lysate
Lane 5 : Raw 264.7 whole cell lysate
Lysates/proteins at 30 µg per lane.
Predicted band size: 33 kDa
12% SDS PAGE
数据表及文件
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SDS download
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Datasheet download
文献 (26)
ab137749 被引用在 26 文献中.
- Wei MF et al. Atorvastatin Attenuates Radiotherapy-Induced Intestinal Damage through Activation of Autophagy and Antioxidant Effects. Oxid Med Cell Longev 2022:7957255 (2022). PubMed: 36092168
- Qu Y et al. The neuroprotection of deproteinized calf blood extractives injection against Alzheimer's disease via regulation of Nrf-2 signaling. Aging (Albany NY) 13:11150-11169 (2021). PubMed: 33819182
- Cano-Cano F et al. Anti-Inflammatory (M2) Response Is Induced by a sp2-Iminosugar Glycolipid Sulfoxide in Diabetic Retinopathy. Front Immunol 12:632132 (2021). PubMed: 33815384
- Tan CT et al. MOAP-1-mediated dissociation of p62/SQSTM1 bodies releases Keap1 and suppresses Nrf2 signaling. EMBO Rep 22:e50854 (2021). PubMed: 33393215
- Yajun W et al. Betaine Attenuates Osteoarthritis by Inhibiting Osteoclastogenesis and Angiogenesis in Subchondral Bone. Front Pharmacol 12:723988 (2021). PubMed: 34658862