Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR7899] to HDGF
- Suitable for: WB, IHC-P, Flow Cyt, ICC/IF
- Reacts with: Human
参阅全部 HDGF 一抗
描述兔单克隆抗体[EPR7899] to HDGF
经测试应用适用于: WB, IHC-P, Flow Cyt, ICC/IFmore details
Synthetic peptide corresponding to residues at the C terminus of Human HDGF (UniProt P51858).
- A549, Saos-2, ECV-304, and 293T cell lysates; A549 cells; Human colon tissue
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
存放说明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol, 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
纯度Tissue culture supernatant
Our Abpromise guarantee covers the use of ab131046 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/10000 - 1/50000. Detects a band of approximately 40 kDa (predicted molecular weight: 27 kDa).|
|IHC-P||1/250 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|Flow Cyt||1/10 - 1/100.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
|ICC/IF||1/250 - 1/500.|
功能Heparin-binding protein, with mitogenic activity for fibroblasts. Acts as a transcriptional repressor.
序列相似性Belongs to the HDGF family.
Contains 1 PWWP domain.
结构域The PWWP domain harbors the heparin-binding sites and is responsible for DNA-binding, while the C-terminal region is essentially unstructured.
翻译后修饰Sumoylated by SUMO1. Sumoylation prevents binding to chromatin.
- Information by UniProt
- FLJ96580 antibody
- HDGF antibody
- HDGF_HUMAN antibody
All lanes : Anti-HDGF antibody [EPR7899] (ab131046) at 1/10000 dilution
Lane 1 : A549 cell lysate
Lane 2 : Saos-2 cell lysate
Lane 3 : ECV-304 cell lysate
Lane 4 : 293T cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 27 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Immunohistochemical analysis of paraffin-embedded Human colon tissue labelling HDGF with ab131046 antibody at 1/250 dilution.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunofluorescence analysis of A549 cells labelling HDGF with ab131046 antibody at 1/250 dilution.