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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation
使用敲除细胞株进行验证重组RabMAb

重组Anti-HDAC9抗体[EPR5223] (ab109446)

  • Datasheet
  • SDS
Reviews (2) Submit a question References (11)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC9 antibody [EPR5223] (ab109446)
  • Western blot - Anti-HDAC9 antibody [EPR5223] (ab109446)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC9 antibody [EPR5223] (ab109446)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC9 antibody [EPR5223] (ab109446)
  • Immunocytochemistry/ Immunofluorescence - Anti-HDAC9 antibody [EPR5223] (ab109446)
  • Immunoprecipitation - Anti-HDAC9 antibody [EPR5223] (ab109446)
  • Flow Cytometry - Anti-HDAC9 antibody [EPR5223] (ab109446)
  • Western blot - Anti-HDAC9 antibody [EPR5223] (ab109446)
  • Anti-HDAC9 antibody [EPR5223] (ab109446)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR5223] to HDAC9
  • Suitable for: IP, Flow Cyt, WB, IHC-P, ICC/IF
  • Knockout validated
  • Reacts with: Mouse, Rat, Human

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概述

  • 产品名称

    Anti-HDAC9抗体[EPR5223]
    参阅全部 HDAC9 一抗
  • 描述

    兔单克隆抗体[EPR5223] to HDAC9
  • 宿主

    Rabbit
  • Tested Applications & Species

    Application Species
    Flow Cyt
    Human
    ICC/IF
    Human
    IHC-P
    Rat
    Human
    IP
    Human
    WB
    Mouse
    Human
    See all applications and species data
  • 免疫原

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • 阳性对照

    • IHC-P: Human colon, rat and mouse cerebrum, ICC/IF: HeLa cells WB: HepG2, Raji, HAP1, cell lysates Flow Cyt: K-562 cells, IP: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate.
  • 常规说明

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
  • 存储溶液

    pH: 7.20
    Preservative: 0.05% Sodium azide
    Constituents: 0.1% BSA, 40% Glycerol (glycerin, glycerine), 9.85% Tris glycine, 50% Tissue culture supernatant
  • Concentration information loading...
  • 纯度

    Tissue culture supernatant
  • 克隆

    单克隆
  • 克隆编号

    EPR5223
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class II / Hda1 Class
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other

相关产品

  • Alternative Versions

    • Alexa Fluor® 488 Anti-HDAC9 antibody [EPR5223] (ab206713)
    • Alexa Fluor® 647 Anti-HDAC9 antibody [EPR5223] (ab207050)
    • Anti-HDAC9 antibody [EPR5223] - BSA and Azide free (ab239979)
  • Compatible Secondaries

    • Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • Hep G2 nuclear extract lysate (ab14660)
    • K-562 nuclear extract lysate (ab14851)
    • Raji whole cell lysate (ab30124)
  • Recombinant Protein

    • Recombinant human HDAC9 protein (ab80350)

应用

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109446 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用 Species
Flow Cyt
Human
ICC/IF
Human
IHC-P
Rat
Human
IP
Human
WB
Mouse
Human
应用 Ab评论 说明
IP
1/5000.
Flow Cyt
Use at an assay dependent concentration.
WB
1/10000 - 1/50000. Detects a band of approximately 160 kDa (predicted molecular weight: 111 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF (1)
1/100 - 1/250.
说明
IP
1/5000.
Flow Cyt
Use at an assay dependent concentration.
WB
1/10000 - 1/50000. Detects a band of approximately 160 kDa (predicted molecular weight: 111 kDa).
IHC-P
1/250 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
ICC/IF
1/100 - 1/250.

靶标

  • 功能

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Represses MEF2-dependent transcription.
    Isoform 3 lacks active site residues and therefore is catalytically inactive. Represses MEF2-dependent transcription by recruiting HDAC1 and/or HDAC3. Seems to inhibit skeletal myogenesis and to be involved in heart development. Protects neurons from apoptosis, both by inhibiting JUN phosphorylation by MAPK10 and by repressing JUN transcription via HDAC1 recruitment to JUN promoter.
  • 组织特异性

    Broadly expressed, with highest levels in brain, heart, muscle and testis. Isoform 3 is present in human bladder carcinoma cells (at protein level).
  • 疾病相关

    Note=A chromosomal aberration involving HDAC9 is found in a family with Peters anomaly. Translocation t(1;7)(q41;p21) with TGFB2 resulting in lack of HDAC9 protein.
  • 序列相似性

    Belongs to the histone deacetylase family. HD type 2 subfamily.
  • 翻译后修饰

    Phosphorylated on Ser-220 and Ser-450; which promotes 14-3-3-binding, impairs interaction with MEF2, and antagonizes antimyogenic activity. Phosphorylated on Ser-240; which impairs nuclear accumulation (By similarity). Isoform 7 is phosphorylated on Tyr-1010. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
    Sumoylated.
  • 细胞定位

    Nucleus.
  • Target information above from: UniProt accession Q9UKV0 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 9734 Human
    • Entrez Gene: 79221 Mouse
    • Entrez Gene: 687001 Rat
    • Omim: 606543 Human
    • SwissProt: Q9UKV0 Human
    • SwissProt: Q99N13 Mouse
    • Unigene: 196054 Human
    • Unigene: 310551 Mouse
    • Unigene: 483009 Mouse
    see all
  • 别名

    • HD 7 antibody
    • HD 7B antibody
    • HD 9 antibody
    • HD7 antibody
    • HD7B antibody
    • HD9 antibody
    • HDAC 7 antibody
    • HDAC 7B antibody
    • HDAC 9 antibody
    • HDAC 9B antibody
    • HDAC 9FL antibody
    • HDAC antibody
    • HDAC7 antibody
    • HDAC7B antibody
    • HDAC9 antibody
    • HDAC9_HUMAN antibody
    • HDAC9B antibody
    • HDAC9FL antibody
    • HDRP antibody
    • Histone deacetylase 4/5 related protein antibody
    • Histone deacetylase 7 antibody
    • Histone deacetylase 7B antibody
    • Histone deacetylase 9 antibody
    • Histone deacetylase 9A antibody
    • Histone deacetylase related protein antibody
    • Histone deacetylase-related protein antibody
    • KIAA0744 antibody
    • MEF2 interacting transcription repressor MITR antibody
    • MEF2 interacting transcription repressor protein antibody
    • MEF2-interacting transcription repressor MITR antibody
    • MITR antibody
    see all

图片

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC9 antibody [EPR5223] (ab109446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC9 antibody [EPR5223] (ab109446)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat Cerebrum tissue labelling HDAC9 with ab109446 at 1/200 dilution for 20 minutes at room temperature. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Nuclear staining on rat cerebrum. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Western blot - Anti-HDAC9 antibody [EPR5223] (ab109446)
    Western blot - Anti-HDAC9 antibody [EPR5223] (ab109446)

    Lane 1: Wild-type HAP1 whole cell lysate (20 µg)
    Lane 2: HDAC9 (KO) knockout HAP1 whole cell lysate (20 µg)
    Lane 3: HepG2 whole cell lysate (20 µg)
    Lane 4: Raji whole cell lysate (20 µg)


    Lanes 1 - 4: Merged signal (red and green). Green - ab109446 observed at 140 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab109446 was shown to specifically recognize HDAC9 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when HDAC9 knockout samples were usedexamined. Wild-type and HDAC9 knockout samples were subjected to SDS-PAGE. Ab109446 and ab8245 (Mouse anti GAPDH loading control) were incubated overnight at 4°C at 1/10,000 dilution and 1/10,000 dilution respectively. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preabsorbed ab216773 and Goat anti-Mouse IgG H&L (IRDye® 680RD) preabsorbed ab216776 secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.


  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC9 antibody [EPR5223] (ab109446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC9 antibody [EPR5223] (ab109446)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse Cerebrum tissue labelling HDAC9 with ab109446 at 1/200 dilution for 20 minutes at room temperature. Heat mediated antigen retrieval was performed with Tris-EDTA buffer (pH 9.0, epitope retrieval solution2) for 20 mins. Nuclear staining on mouse cerebrum. The immunostaining was performed on a Leica Biosystems BOND® RX instrument

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC9 antibody [EPR5223] (ab109446)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC9 antibody [EPR5223] (ab109446)

    ab109446 at 1/250 dilution staining HDAC9 in Human colon by Immunohistochemistry, Paraffin-embedded tissue.

    Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

  • Immunocytochemistry/ Immunofluorescence - Anti-HDAC9 antibody [EPR5223] (ab109446)
    Immunocytochemistry/ Immunofluorescence - Anti-HDAC9 antibody [EPR5223] (ab109446)
    ab109446 at 1/100 dilution staining HDAC9 in HeLa cells by Immunofluorescence.
  • Immunoprecipitation - Anti-HDAC9 antibody [EPR5223] (ab109446)
    Immunoprecipitation - Anti-HDAC9 antibody [EPR5223] (ab109446)

    HDAC9 was immunoprecipitated from 0.35 mg K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate with ab109446 at 1/30 dilution (2ug in 0.35mg lysates). Western blot was performed on the immunoprecipitate using 260035 at 1/1000 dilution. VeriBlot for IP Detection Reagent (HRP)(ab131366) was used at 1/5000 dilution.

    Lane 1: K-562 (human chronic myelogenous leukemia lymphoblast) whole cell lysate 10 μg

    Lane 2: K-562 whole cell lysate

    Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab109446 in K-562 whole cell lysate

    Blocking and dilution buffer and concentration: 5% NFDM/TBST.

    Exposure time: 111 seconds.

  • Flow Cytometry - Anti-HDAC9 antibody [EPR5223] (ab109446)
    Flow Cytometry - Anti-HDAC9 antibody [EPR5223] (ab109446)

    Flow cytometry of K-562 Human chronic myelogenous leukemia lymphoblast cells staining HDAC9 with ab109446 (red) at 1:250. Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used at 1:2000 as a secondary. Rabbit monoclonal IgG (ab172730)  (Black) was used as an isotype control. Cell without incubation with primary antibody and secondary antibody (Blue) used as an unlabelled control. Fixed with 4% paraformaldehyde. Permeabilised with 90% methanol.

  • Western blot - Anti-HDAC9 antibody [EPR5223] (ab109446)
    Western blot - Anti-HDAC9 antibody [EPR5223] (ab109446)
    All lanes : Anti-HDAC9 antibody [EPR5223] (ab109446) at 1/10000 dilution

    Lane 1 : K562 cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : Raji cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 111 kDa
    Observed band size: 160 kDa why is the actual band size different from the predicted?

  • Anti-HDAC9 antibody [EPR5223] (ab109446)
    Anti-HDAC9 antibody [EPR5223] (ab109446)

实验方案

  • Western blot protocols
  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (11)

发表研究结果有使用 ab109446?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab109446 被引用在 11 文献中.

  • Li H  et al. High HDAC9 is associated with poor prognosis and promotes malignant progression in pancreatic ductal adenocarcinoma. Mol Med Rep 21:822-832 (2020). PubMed: 31974610
  • Fu Y  et al. Decreased expression and hypomethylation of HDAC9 lead to poor prognosis and inhibit immune cell infiltration in clear cell renal cell carcinoma. Urol Oncol N/A:N/A (2020). PubMed: 32402768
  • Xu L  et al. MicroRNA-936 inhibits the malignant phenotype of retinoblastoma by directly targeting HDAC9 and deactivating the PI3K/AKT pathway. Oncol Rep 43:635-645 (2020). PubMed: 31922233
  • Luo L  et al. HDAC4 Controls Muscle Homeostasis through Deacetylation of Myosin Heavy Chain, PGC-1a, and Hsc70. Cell Rep 29:749-763.e12 (2019). PubMed: 31618641
  • Li A  et al. Histone deacetylase 6 regulates the immunosuppressive properties of cancer-associated fibroblasts in breast cancer through the STAT3-COX2-dependent pathway. Oncogene N/A:N/A (2018). PubMed: 29980788
View all Publications for this product

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1-2 of 2 Abreviews or Q&A

Immunocytochemistry/ Immunofluorescence abreview for Anti-HDAC9 antibody [EPR5223]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (hiPSC-SMC (smooth muscle cells))
Permeabilization
Yes - 0.05% Triton-X 100
Specification
hiPSC-SMC (smooth muscle cells)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10%
Fixative
Formaldehyde
Read More

Mrs. Alessandra Granata

Verified customer

提交于 Feb 06 2020

Immunohistochemistry (Frozen sections) abreview for Anti-HDAC9 antibody [EPR5223]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry (Frozen sections)
Sample
Rat Tissue sections (Basilar Artery)
Permeabilization
Yes - 0.1% Triton-X PBS
Specification
Basilar Artery
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Fixative
Formaldehyde
Read More

Courtney Fisher

Verified customer

提交于 Apr 19 2017

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