Key features and details
- Rabbit polyclonal to HDAC5
- Suitable for: WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
参阅全部 HDAC5 一抗
特异性The antibody detects ~124 kDa histone deacetylase 5. It does not cross-react with other HDAC proteins including HDAC1, 2, 3, 4, 6, 7, and 8.
经测试应用适用于: WBmore details
种属反应性与反应: Mouse, Rat, Human
Synthetic peptide within Human HDAC5. The exact sequence is proprietary. Synthetic peptide surrounding amino acid 575
Database link: Q9UQL6
常规说明Some tissues may not express the HDAC-4 and 5 protein or it is expressed at extremely low amount. HDAC-1,2,3,6,7 are usually much more abundant (could be ~100X more) than HDAC-4 & 5. We would strongly suggest using positive control (HeLa lysate).
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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存放说明Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 30% Glycerol, 0.5% BSA, PBS
Concentration information loading...
纯度Immunogen affinity purified
Our Abpromise guarantee covers the use of ab1439 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 4 µg/ml. Predicted molecular weight: 121.9 kDa.
HDAC-5 is in very low abundance (~100X lower) in comparison to other HDACs (e.g., HDAC-1, 2, 6, or 7, etc.). For certain tissue and cells, it can be very difficult to detect due to the low abundance, we recommend to try to use strong lysis buffers (containing NP40),load more protein and use more antibody.
功能Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Involved in muscle maturation by repressing transcription of myocyte enhancer MEF2C. During muscle differentiation, it shuttles into the cytoplasm, allowing the expression of myocyte enhancer factors.
序列相似性Belongs to the histone deacetylase family. HD type 2 subfamily.
结构域The nuclear export sequence mediates the shuttling between the nucleus and the cytoplasm.
翻译后修饰Phosphorylated by CaMK at Ser-259 and Ser-498. The phosphorylation is required for the export to the cytoplasm. Phosphorylated by the PKC kinases PKN1 and PKN2, impairing nuclear import.
Ubiquitinated. Polyubiquitination however does not lead to its degradation.
细胞定位Nucleus. Cytoplasm. Shuttles between the nucleus and the cytoplasm. In muscle cells, it shuttles into the cytoplasm during myocyte differentiation. The export to cytoplasm depends on the interaction with a 14-3-3 chaperone protein and is due to its phosphorylation at Ser-259 and Ser-498 by CaMK.
- Information by UniProt
- Antigen NY CO 9 antibody
- Antigen NY-CO-9 antibody
- HD5 antibody
Anti-HDAC5 antibody (ab1439) at 1/1000 dilution + Jurkat (human T cell leukemia cell line from peripheral blood) whole cell lysate at 100 µg
Goat Anti-Rabbit HRP at 1/10000 dilution
Predicted band size: 121.9 kDa
All lanes : Anti-HDAC5 antibody (ab1439) at 1/1000 dilution
All lanes : Rat hippocampus cell lysate
Lysates/proteins at 15 µg per lane.
All lanes : Polyclonal anti-rabbit IgG HRP conjugate at 1/2000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 121.9 kDa
Exposure time: 10 minutes
All lanes : Anti-HDAC5 antibody (ab1439)
Lane 1 : Cell lysate prepared from HeLa cells
Lane 2 : Cell lysate prepared from HepG2 cells
Predicted band size: 121.9 kDa
Observed band size: 124 kDa why is the actual band size different from the predicted?
Western blot analysis of HDAC5 in HeLa (Lane1) and HepG2 (Lane2) cell lysates. A band of approximately 124 KDa can be detected.
ab1439 被引用在 18 文献中.
- Mochalova EP et al. Differences in the Role of HDACs 4 and 5 in the Modulation of Processes Regulating MAFbx and MuRF1 Expression during Muscle Unloading. Int J Mol Sci 21:N/A (2020). PubMed: 32646070
- Tyganov SA et al. Effects of Plantar Mechanical Stimulation on Anabolic and Catabolic Signaling in Rat Postural Muscle Under Short-Term Simulated Gravitational Unloading. Front Physiol 10:1252 (2019). PubMed: 31611819
- Li C et al. Nuclear receptor corepressor 1 represses cardiac hypertrophy. EMBO Mol Med 11:e9127 (2019). PubMed: 31532577
- Wang SE et al. Capsaicin upregulates HDAC2 via TRPV1 and impairs neuronal maturation in mice. Exp Mol Med 50:e455 (2018). PubMed: 29520110
- Li Z et al. HADC5 deacetylates MKL1 to dampen TNF-a induced pro-inflammatory gene transcription in macrophages. Oncotarget 8:94235-94246 (2017). PubMed: 29212224