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Epigenetics and Nuclear Signaling Chromatin Modifying Enzymes Acetylation

Anti-HDAC3抗体(ab7030)

  • Datasheet
  • SDS
Reviews (10)Q&A (5)References (102)

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Western blot - Anti-HDAC3 antibody - ChIP Grade (ab7030)
  • Immunocytochemistry - Anti-HDAC3 antibody - ChIP Grade (ab7030)
  • Western blot - Anti-HDAC3 antibody - ChIP Grade (ab7030)
  • Immunocytochemistry - Anti-HDAC3 antibody (ab7030)

Key features and details

  • Rabbit polyclonal to HDAC3
  • Suitable for: ICC, WB
  • Reacts with: Mouse, Rat, Human, African green monkey, Chinese hamster
  • Isotype: IgG

重组抗体的批间可重复性更强

Product image
Anti-HDAC3 antibody [Y415] (ab32369)
  • 研究可靠 —— 各批次间结果一致且可重复
  • 长期批量供应 —— 采用重组技术,可实现快速生产
  • 首次实验即可成功 —— 经过大量验证确认了特异性
  • 符合伦理标准 —— 产品不含动物成分

概述

  • 产品名称

    Anti-HDAC3抗体
    参阅全部 HDAC3 一抗
  • 描述

    兔多克隆抗体to HDAC3
  • 宿主

    Rabbit
  • 经测试应用

    适用于: ICC, WBmore details
  • 种属反应性

    与反应: Mouse, Rat, Human, African green monkey, Chinese hamster
  • 免疫原

    Synthetic peptide corresponding to Human HDAC3 aa 411-428 conjugated to Keyhole Limpet Haemocyanin (KLH). The epitope recognized by the antibody is resistant to routine formalin-fixation and paraffin-embedding.
    Sequence:

    C-NEFYDGDHDNDKESDVEI

    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • 常规说明

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

性能

  • 形式

    Liquid
  • 存放说明

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • 存储溶液

    pH: 7.40
    Preservative: 0.097% Sodium azide
    Constituent: 0.0268% PBS
  • Concentration information loading...
  • 纯度

    IgG fraction
  • 纯化说明

    Whole antiserum is fractionated and further purified by anion-exchange chromatography to provide the IgG fraction of antiserum that is essentially free of other rabbit serum proteins.
  • 克隆

    多克隆
  • 同种型

    IgG
  • 研究领域

    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • Stem Cells
    • Signaling Pathways
    • Wnt
    • HDACs
    • Epigenetics and Nuclear Signaling
    • Chromatin Modifying Enzymes
    • Acetylation
    • HDACs
    • Class I
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • Other
    • Cardiovascular
    • Heart
    • Hypertrophy
    • Other
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Metabolism of lipids and lipoproteins
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Lipid and lipoprotein metabolism
    • Lipid metabolism
    • Metabolism
    • Types of disease
    • Cancer

相关产品

  • ChIP Related Products

    • Rabbit Anti-Mouse IgG H&L (ab46540)
    • ChIP Kit (ab500)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant human HDAC3 protein (ab101663)

应用

The Abpromise guarantee

Abpromise™承诺保证使用ab7030于以下的经测试应用

“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。

应用 Ab评论 说明
ICC
1/200.
WB (6)
Use a concentration of 2000 - 10000 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).
说明
ICC
1/200.
WB
Use a concentration of 2000 - 10000 µg/ml. Detects a band of approximately 49 kDa (predicted molecular weight: 49 kDa).

靶标

  • 功能

    Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Probably participates in the regulation of transcription through its binding to the zinc-finger transcription factor YY1; increases YY1 repression activity. Required to repress transcription of the POU1F1 transcription factor. Acts as a molecular chaperone for shuttling phosphorylated NR2C1 to PML bodies for sumoylation.
  • 组织特异性

    Widely expressed.
  • 序列相似性

    Belongs to the histone deacetylase family. HD type 1 subfamily.
  • 翻译后修饰

    Sumoylated in vitro.
  • 细胞定位

    Nucleus.
  • Target information above from: UniProt accession O15379 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • 数据库链接

    • Entrez Gene: 8841 Human
    • Entrez Gene: 15183 Mouse
    • Entrez Gene: 84578 Rat
    • Omim: 605166 Human
    • SwissProt: O15379 Human
    • SwissProt: O88895 Mouse
    • SwissProt: Q6P6W3 Rat
    • Unigene: 519632 Human
    • Unigene: 20521 Mouse
    • Unigene: 17284 Rat
    see all
  • 别名

    • HD3 antibody
    • HDAC 3 antibody
    • HDAC3 antibody
    • HDAC3_HUMAN antibody
    • Histone deacetylase 3 antibody
    • RPD3 2 antibody
    • RPD3 antibody
    • RPD3-2 antibody
    • SMAP45 antibody
    see all

图片

  • Western blot - Anti-HDAC3 antibody - ChIP Grade (ab7030)
    Western blot - Anti-HDAC3 antibody - ChIP Grade (ab7030)
    All lanes : Anti-HDAC3 antibody (ab7030) at 1/5000 dilution

    Lane 1 : HEK-293T whole cell lysate.
    Lane 2 : HeLa whole cell lysate.
    Lane 3 : K562 whole cell lysate.
    Lane 4 : COS-7 whole cell lysate.
    Lane 5 : CHO whole cell lysate.
    Lane 6 : NIH-3T3 whole cell lysate.
    Lane 7 : Neuro-2a whole cell lysate.
    Lane 8 : PC-12 whole cell lysate.
    Lane 9 : NRK whole cell lysate.

    Secondary
    All lanes : Goat Anti-Rabbit IgG-Peroxidase and a chemiluminescent substrate.

    Predicted band size: 49 kDa

  • Immunocytochemistry - Anti-HDAC3 antibody - ChIP Grade (ab7030)
    Immunocytochemistry - Anti-HDAC3 antibody - ChIP Grade (ab7030)

    Immunocytochemical immunofluorescence analysis of HeLa cells labelling HDAC3 with ab7030. Cells were fixed and permeabilized with cold methanol followed by a cold methanol and acetone solution. Fixed cells were stained with ab7030 at a 1/200 concentration. The secondary used was a Goat Anti-Rabbit IgG, Cy3 conjugate.

  • Western blot - Anti-HDAC3 antibody - ChIP Grade (ab7030)
    Western blot - Anti-HDAC3 antibody - ChIP Grade (ab7030)This image is courtesy of an anonymous Abreview
    All lanes : Anti-HDAC3 antibody (ab7030) at 1/10000 dilution

    Lane 1 : Whole cell lysate from human HEK293 cell line
    Lane 2 : Whole cell lysate from human HEK293 cell line treated with HDAC3 gene silencing shRNA

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : HRP-conjugated goat anti-rabbit Ig at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 49 kDa


    Exposure time: 10 seconds

    See Abreview

  • Immunocytochemistry - Anti-HDAC3 antibody (ab7030)
    Immunocytochemistry - Anti-HDAC3 antibody (ab7030)This image is courtesy of Michael Mancini, Baylor College of Medicine
    Cells were fixed with 4% formaldehyde in PEM buffer. The coverslip was incubated in blocking buffer of 5% powdered milk in TBS-T plus 0.02% sodium azide for 1 hour at room temperature. Blocking buffer was removed and primary antibody was added at a dilution of 1/5000 and incubated overnight at 4 degrees celsius. The coverslips were then washed 4-5 times with blocking buffer for 5 minutes. Secondary antibody, goat anti-rabbit Alexa 594, was added at a dilution of 1/1000 and incubated at room temperature for one hour. From this point on coverslips were covered with foil to protect them from light. They were washed 5 times with TBS-T and then one time with PEM, for 5 minutes each wash. The coverslips were fixed 10-30 minutes in 4% formaldehyde in PEM buffer, then washed 3 times with PEM buffer for 5 minutes. 0.1M ammonium chloride in PEM buffer was added for 10 minutes to quench auto-florescence, and then slips were washed 2 times for 5 minutes in PEM followed by 3 washes for 5 minutes in

实验方案

  • ChIP protocols
  • Immunoprecipitation protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

数据表及文件

  • SDS download

  • Datasheet download

    Download

文献 (102)

发表研究结果有使用 ab7030?请让我们知道,以便我们可以引用本数据表中的参考文章。

ab7030 被引用在 102 文献中.

  • Cheng S  et al. Romidepsin (FK228) in a Mouse Model of Lipopolysaccharide-Induced Acute Kidney Injury is Associated with Down-Regulation of the CYP2E1 Gene. Med Sci Monit 26:e918528 (2020). PubMed: 31954012
  • Ferrante F  et al. HDAC3 functions as a positive regulator in Notch signal transduction. Nucleic Acids Res 48:3496-3512 (2020). PubMed: 32107550
  • Elmansi AM  et al. Age-related increase of kynurenine enhances miR29b-1-5p to decrease both CXCL12 signaling and the epigenetic enzyme Hdac3 in bone marrow stromal cells. Bone Rep 12:100270 (2020). PubMed: 32395570
  • Sun J  et al. N-terminal truncated carboxypeptidase E represses E-cadherin expression in lung cancer by stabilizing the Snail-HDAC complex. Am J Cancer Res 10:925-938 (2020). PubMed: 32266100
  • Guo Y  et al. NF- ? B/HDAC1/SREBP1c pathway mediates the inflammation signal in progression of hepatic steatosis. Acta Pharm Sin B 10:825-836 (2020). PubMed: 32528830
View all Publications for this product

客户评价及客户问答

Show All 评价 Q&A
提交评价 提交问题

1-10 of 15 Abreviews or Q&A

Question

Inquiry: Dear Scientific Support group, We have been using ab16047 Ab to detect HDAC3 in WB for many years, results are always consistent. I recently performed a ChIP assay using this Ab, but it failed. I know that this Ab is not recommended for ChIP. I found another Ab #ab7030, which is ChIP grade. I also noticed that both of these HDAC3 antibodies are raised against C-terminus of human HDAC3. I was wondering what makes these two Abs so different in terms of ChIP assays, since they both detect the similar region. I was wondering whether you have documented this difference? In addition, which HDAC3 Ab do you recommend for ChIP assay based on your experience? Thanks, Yue

Read More

Abcam community

Verified customer

Asked on Nov 15 2012

Answer

Thank you for contacting us.

While the specific immunogen for both ab16047 and ab7030 are similar, some antibodies work better in certain applications due to the nature in which they are derived, stored, and their specific affinity for the antigen. Specifically, our "ChIP Grade" antibodies such as the ab7030 that you mentioned have been tested and validated to work specifically in ChIP.

I recommend the HDAC3 ChIP Grade antibody that you referenced (ab7030: https://www.abcam.com/hdac3-antibody-chip-grade-ab7030.html) as it has 10 Abreviews with an average rating of 4/5 stars (2 Abreviews for ChIP) and 20 specific references.

For your convencience, I've included links to the 2 ChIP Abreviews for ab7030 below:
https://www.abcam.com/HDAC3-antibody-ab7030.html&tab=abreviews&intabreviewid=25057
https://www.abcam.com/HDAC3-antibody-ab7030.html&tab=abreviews&intabreviewid=18386


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

回复于 Nov 15 2012

Western blot abreview for Anti-HDAC3 antibody - ChIP Grade

Below Average
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Monkey Cell lysate - whole cell (Brain)
Gel Running Conditions
Reduced Denaturing (4-20%)
Loading amount
15 µg
Specification
Brain
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

提交于 May 29 2015

ChIP abreview for Anti-HDAC3 antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
ChIP
Detection step
Real-time PCR
Sample
Mouse Cell lysate - whole cell (Embryonic Stem Cell)
Specification
Embryonic Stem Cell
Negative control
Immuno-precipitation with non-specific IgG antibody.
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 60 minute(s) and 0 second(s)
Specification of the cross-linking agent: formaldehyde
Positive control
WT embryonic stem cells treated with all-trans retinoic acid (1uM)
Read More

Abcam user community

Verified customer

提交于 Feb 04 2015

Question

I just purchased anti-HDAC3 antibody (ab7030) from abcam. The western blot with NIH3T3 cell lysate showes two band, one is a little bigger than 56KDa and the other is a little smalled than 56KDa. I suppose the samller band is what indicate on the datasheet as 49KDa HDAC3 band. Please see the attached picture.

1.Could you please let meknow why there is a non specific band? any other people showed the same result?

2. I am palnning to use this antibody for ChIP experiment, since this is ChIP grade antibody. Do you think it is okay for ChIP experient since there is non specific band?

Read More

Abcam community

Verified customer

Asked on Jun 26 2012

Answer

Thank you for contacting us.

The mouse HDAC3 has 2 isoforms: 27 and 48 kDa (http://www.uniprot.org/uniprot/O88895).
Also, 2 customers had submitted Abreviews for mouse and WB, one of them for 3T3 cells where they also obtained 2 bands (https://www.abcam.com/HDAC3-antibody-ab7030.html&tab=abreviews&intabreviewid=22723).
I am not sure how their blot compares to yours since the MW markers are not indicated, but please check the link.
The other review shows one band at 49 kDa for amouse brain sample:
https://www.abcam.com/HDAC3-antibody-ab7030.html&tab=abreviews&intabreviewid=20928


Thus, the bands you see could be specific to thesample you are using.It might be worthwhile including a positive human or mouse positive control (e.g. Hek293 cells). Please keep in mind that the human protein has a different set of 2 isoforms: 48.8 and 49.1 kDa (http://www.uniprot.org/uniprot/O15379).


As for using the antibody in ChIP:
The antibody has been tested in this application and would be guaranteed. If the 2 bands you are seeing are specific to the NIH3T3 cells, then it would be no problem using the antibody in ChIP.
Please see also the Abreviews submitted by other customers: https://www.abcam.com/HDAC3-antibody-ChIP-Grade-ab7030-reviews.html


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

Read More

Abcam Scientific Support

回复于 Jun 26 2012

ChIP abreview for Anti-HDAC3 antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
ChIP
Sample
Human Cell lysate - whole cell (lymphoblastoid cell line)
Specification
lymphoblastoid cell line
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: Formaldehyde
Detection step
Real-time PCR
Positive control
NFKBIA promoter
Negative control
GAPDH coding sequence
Read More

Abcam user community

Verified customer

提交于 Jul 15 2011

Western blot abreview for Anti-HDAC3 antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Cell lysate - whole cell (3T3)
Loading amount
500000 cells
Specification
3T3
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

提交于 Jan 19 2011

Western blot abreview for Anti-HDAC3 antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Mouse brain)
Loading amount
20 µg
Specification
Mouse brain
Gel Running Conditions
Reduced Denaturing (4-12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Oct 04 2010

Western blot abreview for Anti-HDAC3 antibody - ChIP Grade

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (lymphoblastoid cell line)
Loading amount
20 µg
Specification
lymphoblastoid cell line
Gel Running Conditions
Non-reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Read More

Abcam user community

Verified customer

提交于 Jun 02 2010

ChIP abreview for Anti-HDAC3 antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
ChIP
Sample
Human Cell lysate - nuclear (Jurkat Cells)
Specification
Jurkat Cells
Type
Cross-linking (X-ChIP)
Duration of cross-linking step: 10 minute(s) and 0 second(s)
Specification of the cross-linking agent: 1% formaldehyde
Detection step
Real-time PCR
Positive control
Total Histone 3 antibody from Upstate
Negative control
IgG
Read More

Abcam user community

Verified customer

提交于 May 19 2010

Western blot abreview for Anti-HDAC3 antibody - ChIP Grade

Good
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Human Cell lysate - whole cell (HEK293)
Loading amount
20 µg
Specification
HEK293
Treatment
HDAC3 - shRNA 48 hours
Gel Running Conditions
Reduced Denaturing (10%)
Blocking step
Milk as blocking agent for 18 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
Read More

Abcam user community

Verified customer

提交于 Oct 10 2008

1-10 of 15 Abreviews or Q&A

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