Application
Western blot
Sample
Human Cell lysate - nuclear (U936 cell line (monocytic))
Specification
U936 cell line (monocytic)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/2000
Incubation time
2 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: Southern Biotech: Goat anti-Mouse IgG (H+L chain
Host species: Goat
Clonality: Monoclonal
Conjugation: Alkaline Phosphatase
Host species: Goat
Clonality: Monoclonal
Conjugation: Alkaline Phosphatase
Detection
Detection method
ECF
Exposure
7 minute(s) and 0 second(s)
Bands
Specific: ~58 kDa Non-specific: 32, 33, 80 kDa
Positive control
U937 nuclear extract (non IP)
Additional data
Additional Notes
Excelent WB antibody, no X-reaction with HDAC-1, also excellent for WB od HDAC-2 when used in conjunction with ab7029 for IP
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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提交于 Feb 20 2006