Application
Immunohistochemistry (Frozen sections)
Sample
Human Tissue sections (lymphoma, multiple myeloma, prostate, ovarian)
Specification
lymphoma, multiple myeloma, prostate, ovarian
Fixative
Formaldehyde
Permeabilization
Yes - Target Retrieval Solution
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Other product details
Dilution
1/12000
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 2% BSA in TBS pH 7.6
Secondary antibody
Name
Non-Abcam antibody was used: Envision and DAB (DAKO, K4001, K3468)
Host species: Goat
Clonality: Monoclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Monoclonal
Conjugation: HRP polymer
Additional data
Additional Notes
Heat-induced retrieval was performed using Target Retrieval Solution, Citrate pH 6 (DAKO, S2369), microwave oven at 600W for 18 min. Following treatment slides were left 20 min in the warm buffer before rinsing in running tap water.
Following antigen retrieval, slides were incubated with Peroxidase Blocking Reagent (DAKO, S2001) for 8 min.
Following antigen retrieval, slides were incubated with Peroxidase Blocking Reagent (DAKO, S2001) for 8 min.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Dr. Lena Marquard
Verified customer
提交于 Aug 01 2008