重组Anti-HDAC1抗体[EPR5517(2)] - BSA and Azide free (ab248968)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5517(2)] to HDAC1 - BSA and Azide free
- Suitable for: ICC/IF, IHC-P, ChIC/CUT&RUN-seq, Flow Cyt (Intra), IP, WB
- Knockout validated
- Reacts with: Human
Related conjugates and formulations
概述
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产品名称
Anti-HDAC1抗体[EPR5517(2)] - BSA and Azide free
参阅全部 HDAC1 一抗 -
描述
兔单克隆抗体[EPR5517(2)] to HDAC1 - BSA and Azide free -
宿主
Rabbit -
经测试应用
适用于: ICC/IF, IHC-P, ChIC/CUT&RUN-seq, Flow Cyt (Intra), IP, WBmore details -
种属反应性
与反应: Human -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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阳性对照
- ICC/IF: HeLa cell. Flow Cyt: HeLa cell. ChIC/CUT&RUN-Seq: HeLa cells.
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常规说明
ab248968 is the carrier-free version of ab150399.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
性能
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形式
Liquid -
存放说明
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
存储溶液
pH: 7.2
Constituent: PBS -
无载体
是 -
Concentration information loading...
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纯度
Protein A purified -
克隆
单克隆 -
克隆编号
EPR5517(2) -
同种型
IgG -
研究领域
相关产品
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Alternative Versions
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Assay kits
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Conjugation kits
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
应用
The Abpromise guarantee
Abpromise™承诺保证使用ab248968于以下的经测试应用
“应用说明”部分 下显示的仅为推荐的起始稀释度;实际最佳的稀释度/浓度应由使用者检定。
应用 | Ab评论 | 说明 |
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ICC/IF |
Use at an assay dependent concentration.
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ChIC/CUT&RUN-seq |
Use at an assay dependent concentration.
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
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IP |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration. Predicted molecular weight: 55 kDa.
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说明 |
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ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ChIC/CUT&RUN-seq
Use at an assay dependent concentration. |
Flow Cyt (Intra)
Use at an assay dependent concentration. |
IP
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Predicted molecular weight: 55 kDa. |
靶标
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功能
Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B. -
组织特异性
Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain. -
序列相似性
Belongs to the histone deacetylase family. HD type 1 subfamily. -
翻译后修饰
Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome. -
细胞定位
Nucleus. - Information by UniProt
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数据库链接
- Entrez Gene: 3065 Human
- Omim: 601241 Human
- SwissProt: Q13547 Human
- Unigene: 88556 Human
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别名
- DKFZp686H12203 antibody
- GON 10 antibody
- HD1 antibody
see all
图片
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ChIC/CUT&RUN was performed using a pAG-MNAse at a final concentration of 700 ng/mL, 2 x 10^5 HeLa (Human cervix adenocarcinoma epithelial cell line) cells and 5 µg of ab150399 [EPR5517(2)]. The resulting DNA was sequenced on the Illumina NovaSeq 6000 to a depth of 10 million reads. The negative IgG control ab172730 is also shown.
Additional screenshots of mapped reads can be downloaded here.
The University of Geneva owns patents relevant to ChIC (Chromatin Immuno-Cleavage) methods.
This data was developed using ab150399, the same antibody clone in a different buffer formulation.
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This data was developed using ab150399, the same antibody clone in a different buffer formulation.
Flow cytometric analysis of 4% paraformaldehyde fixed 90% methanol permeabilized HeLa (Human cervix adenocarcinoma epithelial cell) cells labelling HDAC1 with ab150399 at 1/500 dilution (0.1ug) (Red) compared with a Rabbit monoclonal IgG (ab172730) (Black) isotype control and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (Blue). A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/2000 dilution was used as the secondary antibody. -
This data was developed using ab150399, the same antibody clone in a different buffer formulation.
Immunofluorescent analysis of 4% Paraformaldehyde-fixed, 0.1% TritonX-100 permeabilized HeLa cells labelling HDAC1 with ab150399 at 1/100 (5.17 ug/ml) dilution, followed by ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) antibody at 1/1000 2ug/ml dilution (Green). Confocal image showing nuclear staining in HeLa cell line ab195889 Anti-alpha Tubulin mouse monoclonal antibody - Microtubule Marker (Alexa Fluor® 594) was used to counterstain tubulin at 1/200 2.5ug/ml dilution (Red). The Nuclear counterstain was DAPI (Blue).Secondary antibody only control: Secondary antibody is ab150077 Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) at 1000 2ug/ml dilution.
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This data was developed using ab150399, the same antibody clone in a different buffer formulation.
Lane 1 Wild-type HAP1 cell lysate (20 µg)
Lane 2 HDAC1 knockout HAP1 cell lysate (20 µg)
Lane 3 HeLa cell lysate (20 µg)
Lane 4 Human breast carcinoma lysate (20 µg)
Lanes 1 - 4 Merged signal (red and green). Green - ab150399 observed at 65 kDa. Red - loading control, ab8245 observed at 37 kDa.
ab150399 was shown to recognize HDAC1 when HDAC1 knockout samples were used, along with additional cross-reactive bands. Wild-type and HDAC1 knockout samples were subjected to SDS-PAGE. ab150399 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10000 dilution for 1 h at room temperature before imaging.
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This data was developed using ab150399, the same antibody clone in a different buffer formulation.
Purified ab150399 at 1/30 dilution (2µg) immunoprecipitating HDAC1 in Jurkat whole cell lysate.
Lane 1 (input): Jurkat (Human T cell leukemia T lymphocyte) whole cell lysate 10µg
Lane 2 (+): ab150399 + Jurkat whole cell lysate.
Lane 3 (-): Rabbit monoclonal IgG (ab172730) instead of ab150399 in Jurkat whole cell lysate.
VeriBlot for IP Detection Reagent (HRP) (ab131366) (1/1000 dilution) was used for Western blotting.
Blocking Buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM/TBST.
Observed band size: 62 kDa
Faint band above 62kDa could be Sumoylated HDAC1. (PMID: 28186506) -
This data was developed using ab150399, the same antibody clone in a different buffer formulation.
Immunohistochemical analysis of paraffin-embedded Human testis tissue labelling HDAC1 with ab150399 at 1/50 dilution. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
All lanes : Anti-HDAC1 antibody [EPR5517(2)] (ab150399) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Jurkat cell lysate
Lane 3 : MCF7 cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
Predicted band size: 55 kDaThis data was developed using ab150399, the same antibody clone in a different buffer formulation.
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This data was developed using ab150399, the same antibody clone in a different buffer formulation.
Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: HDAC1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human breast carcinoma lysate (20 µg) or K562 lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab150399 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.
This western blot image is a comparison between ab150399 and a competitor's top cited rabbit polyclonal antibody.
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This data was developed using ab150399, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab150399 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
This data was developed using ab150399, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human stomach tissue using ab150399 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
This data was developed using ab150399, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded normal Human colon tissue using ab150399 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
This data was developed using ab150399, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Ovarian carcinoma tissue using ab150399 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. -
This data was developed using ab150399, the same antibody clone in a different buffer formulation.Immunohistochemical analysis of paraffin embedded Human Thyroid gland carcinoma tissue using ab150399 showing +ve staining.
Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
实验方案
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
数据表及文件
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Datasheet download
Certificate of Compliance
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