Name: Anti-HDAC1 antibody [EPR460(2)]
Brand: RabMAb
SKU: ab109411
Rating: 4 (6 reviews)

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Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR460(2)] to HDAC1
Suitable for: IHC-P, WB, Flow Cyt
Knockout validated
Reacts with: Mouse, Human

产品名称

Anti-HDAC1抗体[EPR460(2)]
参阅全部 HDAC1 一抗
描述

兔单克隆抗体[EPR460(2)] to HDAC1
宿主

Rabbit

Tested Applications & Species

Application	Species
Flow Cyt	Human
IHC-P	Human
WB	Mouse Human

See all applications and species data

免疫原

Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
阳性对照
- WB: Jurkat, HeLa, K562, and MCF7 cell lysates. IHC-P: Human colon and tonsil tissues.
常规说明
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

形式

Liquid
存放说明

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
存储溶液

pH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
纯度

Tissue culture supernatant
克隆

单克隆
克隆编号

EPR460(2)
同种型

IgG
研究领域

Alternative Versions
Assay kits
- Histone Deacetylase (HDAC) Activity Assay Kit (Fluorometric) (ab156064)
Compatible Secondaries
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
- HeLa nuclear extract lysate (ab14655)
Recombinant Protein
- Recombinant human HDAC1 protein (ab101661)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab109411 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

应用	Species
ChIP	Human
Flow Cyt	Human
IHC-P	Human
IP	Mouse Human
WB	Mouse Human
All applications	Rat

应用	Ab评论	说明
IHC-P		1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB	(4)	1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
Flow Cyt		1/50.

说明
IHC-P 1/50 - 1/100. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB 1/1000 - 1/10000. Predicted molecular weight: 55 kDa.
Flow Cyt 1/50.

应用说明

Is unsuitable for IP.

功能

Responsible for the deacetylation of lysine residues on the N-terminal part of the core histones (H2A, H2B, H3 and H4). Histone deacetylation gives a tag for epigenetic repression and plays an important role in transcriptional regulation, cell cycle progression and developmental events. Histone deacetylases act via the formation of large multiprotein complexes. Deacetylates SP proteins, SP1 and SP3, and regulates their function. Component of the BRG1-RB1-HDAC1 complex, which negatively regulates the CREST-mediated transcription in resting neurons. Upon calcium stimulation, HDAC1 is released from the complex and CREBBP is recruited, which facilitates transcriptional activation. Deacetylates TSHZ3 and regulates its transcriptional repressor activity. Deacetylates 'Lys-310' in RELA and thereby inhibits the transcriptional activity of NF-kappa-B.
组织特异性

Ubiquitous, with higher levels in heart, pancreas and testis, and lower levels in kidney and brain.
序列相似性

Belongs to the histone deacetylase family. HD type 1 subfamily.
翻译后修饰

Sumoylated on Lys-444 and Lys-476; which promotes enzymatic activity. Desumoylated by SENP1.
Phosphorylation on Ser-421 and Ser-423 promotes enzymatic activity and interactions with NuRD and SIN3 complexes.
Ubiquitinated by CHFR, leading to its degradation by the proteasome.
细胞定位

Nucleus.
Target information above from: UniProt accession Q13547 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
数据库链接
- Entrez Gene: 3065 Human
- Entrez Gene: 433759 Mouse
- Entrez Gene: 297893 Rat
- Omim: 601241 Human
- SwissProt: Q13547 Human
- SwissProt: O09106 Mouse
- SwissProt: Q4QQW4 Rat
- Unigene: 88556 Human
- Unigene: 202504 Mouse
- Unigene: 391033 Mouse
- Unigene: 1863 Rat
see all
别名
- DKFZp686H12203 antibody
- GON 10 antibody
- HD1 antibody
- HDAC 1 antibody
- HDAC1 antibody
- HDAC1_HUMAN antibody
- Histone deacetylase 1 antibody
- Reduced potassium dependency yeast homolog like 1 antibody
- RPD3 antibody
- RPD3L1 antibody
see all

Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: HDAC1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human breast carcinoma lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109411 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab109411 was shown to recognize HDAC1 when HDAC1 knockout samples were used, along with additional cross-reactive bands. Wild-type and HDAC1 knockout samples were subjected to SDS-PAGE. ab109411 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed withGoat anti-Rabbit IgG H&L (IRDye^® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 h at room temperature before imaging
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

Immunohistochemical analysis of HDAC1 in paraffin-embedded Human colon tissue using ab109411 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Flow Cytometry - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

Overlay histogram showing HeLa cells stained with ab109411 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab109441, 1/50 dilution) for 30 min at 22°C. The secondary antibody used was Alexa Fluor® 488 goat anti-rabbit IgG (H&L) (ab150081) at 1/4000 dilution for 30 min at 22°C.

Isotype control antibody (black line) was rabbit IgG (monoclonal) (ab172730, 0.1ug/1x10⁶ cells) used under the same conditions.

Acquisition of >5,000 events were collected using an 50 mW Blue laser (488nm) with 530/30 bandpass filter.
Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: HDAC1 knockout HAP1 cell lysate (20 µg)
Lane 3: HeLa cell lysate (20 µg)
Lane 4: Human breast carcinoma lysate (20 µg) or K562 lysate (20 µg)
Lanes 1 - 4: Merged signal (red and green). Green - ab109411 observed at 65 kDa. Red - loading control, ab8245, observed at 37 kDa.

This western blot image is a comparison between ab109411 and a competitor's top cited rabbit polyclonal antibody.
Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

All lanes : Anti-HDAC1 antibody [EPR460(2)] (ab109411) at 1/1000 dilution

Lane 1 : Mouse brain lysate, 20ug
Lane 2 : Mouse heart lysate, 20ug
Lane 3 : Mouse Kidney lysate, 20ug
Lane 4 : Mouse spleen lysate,20ug

Lysates/proteins at 20 µg per lane.

Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated(Pierce). at 1/1000 dilution

Predicted band size: 55 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted?

Exposure time: 3 minutes

Blocking and Diluting buffer: 5% NFDM /TBST
Western blot - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

All lanes : Anti-HDAC1 antibody [EPR460(2)] (ab109411) at 1/1000 dilution

Lane 1 : Jurkat cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : K562 cell lysate
Lane 4 : MCF7 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 55 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-HDAC1 antibody [EPR460(2)] (ab109411)

Immunohistochemical analysis of HDAC1 in paraffin-embedded Human tonsil tissue using ab109411 at 1/50 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-HDAC1 antibody [EPR460(2)] (ab109411)

Flow cytometry protocols
Immunohistochemistry protocols
Western blot protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

发表研究结果有使用 ab109411？请让我们知道，以便我们可以引用本数据表中的参考文章。

ab109411 被引用在 16 文献中.

Xiong W et al. MiR-761 inhibits colorectal cancer cell proliferation and invasion through targeting HDAC1. Pharmazie 74:111-114 (2019). PubMed: 30782261
Bartosz P et al. Benzophenone-3 passes through the blood-brain barrier, increases the level of extracellular glutamate and induces apoptotic processes in the hippocampus and frontal cortex of rats. Toxicol Sci N/A:N/A (2019). PubMed: 31368502
Loktev SS & Ogneva IV DNA Methylation of Mouse Testes, Cardiac and Lung Tissue During Long-Term Microgravity Simulation. Sci Rep 9:7974 (2019). PubMed: 31138883
King HW et al. Polycomb repressive complex 1 shapes the nucleosome landscape but not accessibility at target genes. Genome Res 28:1494-1507 (2018). PubMed: 30154222
Sikorski K et al. A high-throughput pipeline for validation of antibodies. Nat Methods 15:909-912 (2018). PubMed: 30377371

View all Publications for this product

客户评价及客户问答

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1-6 of 6 Abreviews or Q&A

Application

Western blot

Sample

Human Cell lysate - whole cell (Brain)

Gel Running Conditions

Reduced Denaturing (4-20%)

Loading amount

12 µg

Treatment

Aliquoted and heated from 34C to 69C to determine melting temperature of HDAC1. Lysate centrifuged at 18,000 x g to remove aggregates after melting. Supernatent collected, 3X SDS PAGE loading dye added, and heated sampled were heated to 95C for 10 min. Note, input sample was not heated before centrifugation, but was heated to 95C after loading dye added.

Specification

Brain

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C

Abcam user community

Verified customer

提交于 Aug 17 2015

Application

Western blot

Loading amount

15 µg

Gel Running Conditions

Reduced Denaturing (4-12% Tris-bis glycine gel)

Sample

Mouse Tissue lysate - other (mouse brain tissue)

Specification

mouse brain tissue

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Arjang Salehi

Verified customer

提交于 Mar 26 2015

Application

Western blot

Loading amount

10 µg

Gel Running Conditions

Reduced Denaturing (10%)

Sample

Human Cell lysate - nuclear (B cells)

Specification

B cells

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Abcam user community

Verified customer

提交于 Mar 16 2015

Application

Western blot

Loading amount

20 µg

Gel Running Conditions

Reduced Denaturing (10%)

Sample

Human Cell lysate - whole cell (heart)

Specification

heart

Blocking step

Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

提交于 Jan 13 2015

Application

Immunocytochemistry/ Immunofluorescence

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 28°C

Sample

Mouse Cell (Neural cell)

Specification

Neural cell

Permeabilization

Yes - 0.3% TritonX-100 in PBS for 30 minutes

Fixative

Paraformaldehyde

Abcam user community

Verified customer

提交于 Oct 13 2014

Application

Immunocytochemistry/ Immunofluorescence

Sample

Human Cell (HeLa)

Specification

HeLa

Fixative

Paraformaldehyde

Permeabilization

Yes - 0.5% Triton-X100 in PBS

Dr. Kirk Mcmanus

Verified customer

提交于 May 31 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

概述

产品名称

描述

宿主

Tested Applications & Species

免疫原

阳性对照

常规说明

性能

形式

存放说明

存储溶液

纯度

克隆

克隆编号

同种型

研究领域

相关产品

Alternative Versions

Assay kits

Compatible Secondaries

Isotype control

Positive Controls

Recombinant Protein

应用

The Abpromise guarantee

靶标

功能

组织特异性

序列相似性

翻译后修饰

细胞定位

数据库链接

别名

图片

实验方案

数据表及文件

SDS download

Datasheet download

文献 (16)

客户评价及客户问答

Western blot abreview for Anti-HDAC1 antibody [EPR460(2)]

Western blot abreview for Anti-HDAC1 antibody [EPR460(2)]

Western blot abreview for Anti-HDAC1 antibody [EPR460(2)]

Western blot abreview for Anti-HDAC1 antibody [EPR460(2)]

Immunocytochemistry/ Immunofluorescence abreview for Anti-HDAC1 antibody [EPR460(2)]

Immunocytochemistry/ Immunofluorescence abreview for Anti-HDAC1 antibody [EPR460(2)]